Abstract
Previous findings vary regarding the timing and cause of elastin fiber degeneration in the elastaseinduced rat abdominal aortic aneurysm model. We examined the timing and cause of elastin fiber degeneration after elastase infusion using two different elastase infusion times. Twenty-four Sprague-Dawley rats were divided into two groups. The infrarenal abdominal aorta was infused with 15 U of elastase for 15 min (n=12, 15-min infusion group) or 30min (n=12, 30-min infusion group). In each group, three rats were killed immediately and 1, 3, and 7 days after infusion, and then the aortas were excised for a histologic examination. Elastin fibers did not stain, even immediately after elastase infusion, in the 30-min infusion group. The degeneration of elastin fibers did not progress in the 15-min infusion group during the period of observation. Inflammatory cells infiltrated mainly to the adventitia near regions where the degeneration of elastin fibers spread totally through the aortic media. Elastin fibers degenerate immediately after elastase infusion and thus seem to degenerate not due to endogenous proteinases that are produced by the infiltrating cells, but due to the exogenously infused elastase itself. Inflammatory cell infiltration was thus found to be a result of the degeneration of elastin fibers in this model.
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References
Anidjar S, Salzmann J, Gentric D, Lagneau P, Camilleri J, Michel J (1990) Elastase-induced experimental aneurysms in rats. Circulation 82:973–981
Halpern VJ, Nackman GB, Gandhi RH, Irizarry E, Scholes JV, Ramey WG, Tilson MD (1994) The elastase infusion model of experimental aortic aneurysm: synchrony of induction of endogenous proteinases with matrix destruction and inflammatory cell response. J Vasc Surg 20:51–60
Holmes DR, Petrinec D, Wester W, Thompson RW, Reilly JM (1996) Indomethacine prevents elastase-induced abdominal aortic aneurysm in the rat. J Surg Res 63:305–309
Curci JA, Petrinec D, Liao S, Golbu LM, Thompson RW (1998) Pharmacologic suppression of experimental abdominal aortic aneurysm: a comparison of doxycycline and four chemically modified tetracyclines. J Vasc Surg 28:1082–1093
Bigatel DA, Elmore JR, Carey DA, Dizmeci-Smith G, Franklin DP, Youkey JR (1999) The matrix metalloproteinases inhibitor BB-94 limits expansion of experimental abdominal aortic aneurysms. J Vasc Surg 29:130–138
Anidjar S, Osborne-Pellegrin M, Coutard M, Michel J (1992) Arterial hypertension and aneurysmal dilatation. Kidney Int 41:S61-S66
Gadowski GR, Ricci MA, Hendley ED, Pilcher DV (1993) Hypertension accelerates the growth of experimental aortic aneurysms. J Surg Res 54:431–436
Ricci MA, Slaiby JM, Gadowski GR, Hendley ED, Nichols P, Pilcher DB (1996) Effects of hypertension and propranolol upon aneurysm expansion in the Anidjar/Dobrin aneurysm model. Ann NY Acad Sci 800:89–96
Ricci MA, Strindberg G, Slaiby JM, Guibord R, Bergersen IJ, Nichols P, Hendry ED, Pilcher DB (1996) Anti-CD 18 monoclonal antibody slows experimental aortic aneurysm expansion. J Vasc Surg 23:301–307
Dobrin PB, Baumgartner N, Anidjar S, Chejfec G, Mrkvicka R (1996) Inflammatory aspects of experimental aneurysms. Effect of methylpredonisolone and cyclosporine. Ann NY Acad Sci 800:74–88
Petrinec D, Liao S, Holmes DR, Reilly JM, Parks WC, Thompson RW (1996) Doxycycline inhibition of aneurysmal degeneration in an elastase-induced rat model of abdominal aortic aneurysm: preservation of aortic elastin associated with suppressed production of 92 kD gelatinase. J Vasc Surg 23:336–346
Nackman GB, Karkowski FJ, Harpern VJ, Gaetz HP, Tilson MD (1997) Elastin degradation products induce adventitial angiogenesis in the Anidjar/Dobrin rat aneurysm model. Surgery 122:39–44
Hingorani A, Ascher E, Scheinman M, Yorkovich W, Depippo P, Ladoulis CT, Selles-Cunha S (1998) The effect of tumor necrosis factor binding protein and interleukin-1 receptor antagonist on the development of abdominal aortic aneurysms in a rat model. J Vasc Surg 28:522–526
Anidjar S, Dobrin PB, Eichorst M, Graham GP, Chejfec G (1992) Correlation of inflammatory infiltrate with the enlargement of experimental aortic aneurysms. J Vasc Surg 16:139–147
Anidjar S, Dobrin PB, Chejfec G, Michel J (1994) Experimental study of determinants of aneurysmal expansion of the abdominal aorta. Ann Vasc Surg 8:127–136
Yamaguchi T, Yokokawa M, Suzuki M, Higashide S, Katoh Y, Sugiyama S, Misaki T (1999) Shortened elastase infusion time in the elastase-induced rat aneurysm model. J Surg Res 85:158–162
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Yamaguchi, T., Yokokawa, M., Suzuki, M. et al. The time course of elastin fiber degeneration in a rat aneurysm model. Surg Today 30, 727–731 (2000). https://doi.org/10.1007/s005950070085
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DOI: https://doi.org/10.1007/s005950070085