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Detection of type III collagen fragments in specimens of abdominal aortic aneurysms

Surgery Today volume 28, pages 385–390 (1998)Cite this article

Abstract

The purpose of this study was to analyze the collagens in aortic aneurysm walls and to investigate the mechanism of the formation of calcified abdominal aortic aneurysms (AAAs). Collagens were extracted from human aneurysmal aortic walls obtained during surgery, and from human nonaneurysmal aortic walls obtained at autopsy, using pepsinacetic acid digestion. Electrophoresis and immunoblotting were performed. Type III collagen was found to be reduced in the arteriosclerotic aneurysmal aortic walls. The α1 chain of type III collagen/α1 chain of type I collagen ratio was 0.35 ±0.11 in the aortic aneurysms and 0.68 ± 0.11 in the nonaneurysmal aortic walls (P=0.0111). All the calcified aneurysms were associated with type III collagen fragments having molecular mass of approximately 70 kDa and 30 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thus, we concluded that AAAs with calcification may be caused by an abnormal degradation of type III collagen.

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Affiliations

  1. First Department of Surgery, Yamaguchi University School of Medicine, 1144 Kogushi, 755, Ube, Yamaguchi, Japan

    Takayuki Kuga, Kensuke Esato, Nobuya Zempo & Kentaro Fujioka

  2. First Department of Biochemistry, Yamaguchi University School of Medicine, 1144 Kogushi, 755, Ube, Yamaguchi, Japan

    Kazuyuki Nakamura

Authors
  1. Takayuki Kuga
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  2. Kensuke Esato
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  3. Nobuya Zempo
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  4. Kentaro Fujioka
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  5. Kazuyuki Nakamura
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Kuga, T., Esato, K., Zempo, N. et al. Detection of type III collagen fragments in specimens of abdominal aortic aneurysms. Surg Today 28, 385–390 (1998). https://doi.org/10.1007/s005950050146

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  • DOI: https://doi.org/10.1007/s005950050146

Key Words

  • abdominal aortic aneurysm
  • calcification
  • type III collagen
  • aortic wall remodeling