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Intestinal mucosa staple line integrity and anastomotic leak pressure after healing in a porcine model

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Abstract

Purpose

The aim of this study was to evaluate both the intestinal mucosa staple line integrity and anastomotic leak pressure after healing in a porcine survival model.

Methods

We used two suture models using two different size staples (incomplete mucosal closure model: group G [staple height 0.75 mm], complete mucosal closure model: group B [staple height 1.5 mm]) in the porcine ileum. Five staple lines were created in each group made in the ileum for each model, and the staple sites harvested on days 0, 2, and 7. The leak pressure at the staple site was measured at each time point.

Results

On day 0, the leak pressure for group G (79.5 mmHg) was significantly lower than that for group B (182.3 mmHg) (p < 0.01). On days 2 and 7, there was no significant difference between groups G and B (171 mmHg and 175.5 mmHg on day 2, 175.5 mmHg and 175.5 mmHg on day 7, p > 0.05). The histological findings in both groups showed similar healing at postoperative days 2 and 7.

Conclusion

The integrity of the mucosal staple lines was associated with the postoperative leak pressure on day 0. However, there was no association with the leak pressure at two days or more postoperatively in a porcine model.

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Acknowledgements

We thank Takuya Ito and Kahori Igarashi for their expert assistance in animal care, and Dr. Yuko Homma and Dr. Toshiki Mimura for providing invaluable suggestions.

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Authors and Affiliations

  1. Department of Surgery, Division of Gastroenterological, General and Transplant Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan

    Daishi Naoi, Hisanaga Horie, Koji Koinuma, Yuko Kumagai, Gaku Ota, Mineyuki Tojo, Yuji Kaneda, Shuji Hishikawa, Ai Sadatomo, Yoshiyuki Inoue, Alan Kawarai Lefor & Naohiro Sata

  2. Department of Pathology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke , Tochigi, 329-0498, Japan

    Noriyoshi Fukushima

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  1. Daishi Naoi
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Naoi, D., Horie, H., Koinuma, K. et al. Intestinal mucosa staple line integrity and anastomotic leak pressure after healing in a porcine model. Surg Today 51, 1713–1719 (2021). https://doi.org/10.1007/s00595-021-02267-9

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  • DOI: https://doi.org/10.1007/s00595-021-02267-9

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