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A new porcine model of ascending aortic aneurysm established using a cover-then-cut method

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Abstract

Purpose

To establish a reproducible porcine model of ascending aortic aneurysm (AsAA).

Methods

We created an AsAA in twelve domestic pigs using a cover-then-cut method, stitching a glutaraldehyde tanned bovine pericardial patch onto the anterolateral aortic wall, then cutting off aortic wall tissue within the patch, and closing the incision in the patch.

Results

The AsAA creation was completed successfully in 11 out of the 12 pigs (91.7%). The mean maximal diameter of the ascending aorta increased significantly by 1.77 times, immediately after the operation (p < 0.05). Significantly continuous expansion of the aneurysm was observed during the follow up period, to 48.9 ± 1.80 mm at 3 months and 50.3 ± 2.25 mm at 6 months (p < 0.05).

Conclusions

This porcine model of AsAA, created using a repeatable cover-then-cut method, represents a reproducible platform for studying and training, which could contribute to the development of new feasible endovascular treatments of AsAAs.

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Abbreviations

AsAA:

Ascending aortic aneurysm

CTA:

Computed tomography angiography

TAA:

Thoracic aortic aneurysm

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Acknowledgements

The study was conceived by Jian Zhou, Jiaxuan Feng and Zaiping Jing. Analysis was by Zhenjiang Li. The manuscript was drafted by Zhenjiang Li, Junjun Liu, and Rui Feng. All authors contributed to analysis and interpretation of the data and critical revision of the manuscript.

Funding

Supported by the National Natural Science Foundation of China (Grant nos. 81770476, 81870366, 91739110, 81970208, 82000430), the Foundation of Shanghai Science and Technology Commission (Grant no. 16411966400), and the Shanghai Science and Technology Support Project (Grant no. 17441901900).

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Author notes

  1. Zhenjiang Li, Junjun Liu and Rui Feng contributed equally to this work.

Authors and Affiliations

  1. Department of Vascular Surgery, Changhai Hospital, Navy Medical University, 168 Changhai Road, Shanghai, 200433, China

    Zhenjiang Li, Junjun Liu, Rui Feng, Jiaxuan Feng, Yiming Li, Xianhao Bao, Feng Qin, Tao Li, Jian Zhou & Zaiping Jing

  2. Department of Vascular Surgery, The First Affiliated Hospital of the Medical School of Zhejiang University, Hangzhou, China

    Zhenjiang Li

  3. Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China

    Junjun Liu

  4. Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, China

    Feng Qin

Authors
  1. Zhenjiang Li
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  2. Junjun Liu
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  3. Rui Feng
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  4. Jiaxuan Feng
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  8. Tao Li
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Contributions

Conception and design: JF, JZ, ZJ. Analysis and interpretation of data: ZL, JL, RF, JF, XB, FQ. Writing the article: ZL, JL, RF. Critical revision of the article: JF, JZ, RF, ZJ. Statistical analysis: ZL, TL. Obtained funding: JF, JZ, ZJ. ZL, JL, and RF participated equally and share equal authorship.

Corresponding authors

Correspondence to Jiaxuan Feng, Jian Zhou or Zaiping Jing.

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We have no conflicts of interest to declare.

Research involving human/animal participation

No human studies were carried out by the authors in relation to this article.

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All institutional and national guidelines for the care and use of laboratory animals were followed and approval was given by the appropriate institutional committees.

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Li, Z., Liu, J., Feng, R. et al. A new porcine model of ascending aortic aneurysm established using a cover-then-cut method. Surg Today 51, 906–915 (2021). https://doi.org/10.1007/s00595-020-02198-x

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  • DOI: https://doi.org/10.1007/s00595-020-02198-x

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