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Collagen gel droplet-embedded culture drug sensitivity test (CD-DST) predicts the effect of adjuvant chemotherapy on pancreatic cancer

  • Kyohei Ariake
  • Fuyuhiko MotoiEmail author
  • Masamichi Mizuma
  • Hideo Ohtsuka
  • Hiroki Hayashi
  • Kei Nakagawa
  • Tatsuo Hata
  • Katsutaka Mitachi
  • Takeshi Naitoh
  • Takashi Kamei
  • Michiaki Unno
Original Article
  • 47 Downloads

Abstract

Purpose

We evaluated the clinical effectiveness of collagen gel droplet-embedded culture drug sensitivity tests (CD-DSTs) in predicting the efficacy of adjuvant chemo-therapeutic treatments for pancreatic cancer (PC).

Methods

The clinicopathological characteristics and prognoses of 22 PC patients who underwent CD-DST after pancreatectomy at Tohoku University between 2012 and 2016 were analyzed retrospectively. Eligibility criteria were resectable or borderline resectable PC, successful evaluation for 5-fluorouracil sensitivity by CD-DST, treatment with S-1 adjuvant chemotherapy, and no preoperative chemotherapy.

Results

The rate of successful evaluation by CD-DST was 52.3% in PC. The optimal T/C ratio, defined as the ratio of the number of cancer cells in the treatment group (T) to that in the control group (C), for 5-fluorouracil was 85% using receiver operating characteristic curve analysis. The sensitive group (T/C ratio < 85%; n = 11) had a better recurrence-free survival rate than the resistant group (T/C ratio ≥ 85%; n = 11; P = 0.029). A Cox proportional hazards regression model demonstrated that sensitivity to 5-fluorouracil was an independent predictor of recurrence on multivariate analysis (hazard ratio 3.28; 95.0% CI 1.20–9.84; P = 0.020).

Conclusions

CD-DSTs helped to predict PC recurrence after S-1 adjuvant chemotherapy.

Keywords

Pancreatic cancer Chemosensitivity test CD-DST 

Notes

Funding

This work was supported in part by a Grant-in-Aid for Scientific Research (24592018) from the Japan Society for the Promotion of Science.

Compliance with ethical standards

Conflict of interest

We have no conflicts of interest to disclose and we did not receive any financial support in relation to this study.

Supplementary material

595_2019_1842_MOESM1_ESM.docx (18 kb)
Supplementary file1 (DOCX 18 kb)
595_2019_1842_MOESM2_ESM.docx (17 kb)
Supplementary file2 (DOCX 16 kb)
595_2019_1842_MOESM3_ESM.docx (19 kb)
Supplementary file3 (DOCX 18 kb)
595_2019_1842_MOESM4_ESM.jpg (200 kb)
Receiver operating characteristic curve of the T/C ratios for predicting recurrence. At the intersection closest to the upper left corner (T/C ratio = 85%), the sensitivity and specificity were 61.1% and 100%, respectively. AUC, area under the curve (JPEG 200 kb)

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Kyohei Ariake
    • 1
  • Fuyuhiko Motoi
    • 1
    Email author
  • Masamichi Mizuma
    • 1
  • Hideo Ohtsuka
    • 1
  • Hiroki Hayashi
    • 1
  • Kei Nakagawa
    • 1
  • Tatsuo Hata
    • 1
  • Katsutaka Mitachi
    • 1
  • Takeshi Naitoh
    • 1
  • Takashi Kamei
    • 1
  • Michiaki Unno
    • 1
  1. 1.Department of SurgeryTohoku University Graduate School of MedicineSendaiJapan

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