Surgery Today

, Volume 48, Issue 5, pp 510–516 | Cite as

Nectin-1 expression in cancer-associated fibroblasts is a predictor of poor prognosis for pancreatic ductal adenocarcinoma

  • Misuzu Yamada
  • Kenichi Hirabayashi
  • Aya Kawanishi
  • Atsuko Hadano
  • Yumi Takanashi
  • Hideki Izumi
  • Yoshiaki Kawaguchi
  • Tetsuya Mine
  • Naoya Nakamura
  • Toshio Nakagohri
Original Article
  • 120 Downloads

Abstract

Purpose

Nectin-1 is a cell adhesion molecule that regulates the formation of adherens junctions and tight junctions. We measured the expression of nectin-1 in cancer-associated fibroblasts (CAFs) in patients with pancreatic ductal adenocarcinoma (PDAC).

Methods

Nectin-1 expression was measured via immunohistochemistry using tissue microarray blocks constructed from resected PDAC tissue from 258 patients. We screened for associations between nectin-1 expression and clinicopathological parameters. According to the percentage of CAFs stained, expression was classified as negative at ≤ 30% and positive at > 30%.

Results

Nectin-1 expression was confirmed in CAFs from 64 patients (24.8%), and was associated with lymph node metastasis (p = 0.016), advanced Union for International Cancer Control stage (p = 0.016), perineural invasion (p = 0.022), pancreatic head tumors (p = 0.023), and shorter overall survival (p = 0.003). Multivariate analysis revealed that nectin-1 expression in CAFs was an independent prognostic factor (p = 0.038).

Conclusions

Diffuse nectin-1 expression in the CAFs of PDAC patients is associated with invasion, metastasis, and shorter survival.

Keywords

Pancreatic ductal adenocarcinoma Nectin-1 Cell adhesion molecules Cancer-associated fibroblast Epithelial mesenchymal transition 

Notes

Acknowledgements

We thank the Division of Diagnostic Pathology, Tokai University Hospital, and the Support Center for Medical Research and Education, Tokai University for their valuable technical assistance.

Compliance with ethical standards

Ethics approval

The study protocol was approved by the Research Ethics Committee (no. 13R244) of the Tokai University School of Medicine (Kanagawa, Japan) and has therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

Conflict of interest

We declare that we have no conflicts of interest.

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Copyright information

© Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Misuzu Yamada
    • 1
  • Kenichi Hirabayashi
    • 2
  • Aya Kawanishi
    • 3
  • Atsuko Hadano
    • 3
  • Yumi Takanashi
    • 2
  • Hideki Izumi
    • 1
  • Yoshiaki Kawaguchi
    • 3
  • Tetsuya Mine
    • 3
  • Naoya Nakamura
    • 2
  • Toshio Nakagohri
    • 1
  1. 1.Department of SurgeryTokai University School of MedicineIseharaJapan
  2. 2.Department of PathologyTokai University School of MedicineIseharaJapan
  3. 3.Department of Gastroenterology and HepatologyTokai University School of MedicineIseharaJapan

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