An increased number of PD-1+ and Tim-3+ CD8+ T cells is involved in immune evasion in gastric cancer
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Co-signaling molecules play an important role in T cells. This study was designed to investigate PD-1 and Tim-3 expression on T cells and the relationships between PD-1 and Tim-3 expression and immune evasion in patients with gastric cancer.
Using multicolor flow cytometry, we analyzed PD-1 and Tim-3 expression on CD8+ T cells obtained from peripheral blood mononuclear cells (PBMCs) and gastric cancer tissue.
Significantly more PD-1+ and Tim-3+ CD8+ T cells in peripheral blood were found in gastric cancer patients than in healthy controls. PD-1+ CD8+ T cells were significantly correlated with Tim-3+ CD8+ T cells in peripheral blood from the gastric cancer patients (r = 0.29, p = 0.036). Furthermore, significantly greater numbers of PD-1+ and Tim-3+ CD8+ T cells were seen in the gastric cancer tissue samples than in the PBMCs. CD8+ T cells positive for both PD-1 and Tim-3 produced significantly less IFN-gamma than cells negative for both and cells positive for PD-1 and negative for Tim-3.
An increased number of PD-1+ and Tim-3+ CD8+ T cells is closely related to impaired function of CD8+ T cells in gastric cancer patients.
KeywordsGastric cancer PD-1 T cell Tim-3
We thank Ms. Miyauchi and Dr. Nakayama (Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University) for their help with cell sorting. This work was supported by JSPS KAKENHI Grant Number 23501285.
Compliance with ethical standards
Conflict of interest
We report no proprietary or commercial interests in any product mentioned or concept discussed in this article.
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