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Early-phase peritoneal drainage and lavage in a rat model of severe acute pancreatitis

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Abstract

Purpose

To evaluate the effects of early-phase drainage on the survival rates and pancreatic pathological changes associated with severe acute pancreatitis (SAP) in a rat model.

Methods

Sprague–Dawley rats were divided into the following groups: SAP model (control), early drainage and delayed drainage. The 24-h survival rates were compared among the groups. In addition, the serum and ascites concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10 and tumor necrosis factor (TNF)-α were measured, and pancreatic pathological changes were observed.

Results

The survival rate significantly improved in the early drainage group. Compared with that observed in the control group, the serum TNF-α and IL-8 concentrations in the early drainage group decreased, while the serum IL-10 levels increased, and the ascites concentrations of IL-1β, IL-6, IL-8 and TNF-α decreased, while that of IL-10 increased significantly. In the delayed drainage group, only the ascites concentrations of TNF-α decreased. Meanwhile, the pancreatic pathological changes at 3, 6 and 24 h worsened in the early drainage group; however, the pancreatic lesions in the early drainage group were less mild than those seen in the control group.

Conclusions

Rebalancing the cytokine levels in ascites after early drainage may be a key factor for enhancing the survival rate in rats.

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Acknowledgments

This study was funded by the Scientific Research Fund of the Shanghai Municipal Health Bureau (No. 2010144). All authors contributed to the intellectual content and approved the final version of the manuscript.

Conflict of interest

No benefits in any form have been or will be received from any commercial parties related directly or indirectly to the subject of this article.

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Authors and Affiliations

  1. Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai, 200336, China

    Leiming Zhu & Peng Sun

  2. Department of Pathology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai, 200336, China

    Jing Yang

  3. Department of General Surgery, No. 1 Hospital, Shanghai Jiao Tong University School of Medicine, 85 Wujin Road, Shanghai, 200080, China

    Jilin Lu

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  1. Leiming Zhu
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Correspondence to Leiming Zhu.

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Zhu, L., Lu, J., Yang, J. et al. Early-phase peritoneal drainage and lavage in a rat model of severe acute pancreatitis. Surg Today 46, 371–378 (2016). https://doi.org/10.1007/s00595-015-1172-9

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