Abstract
The aim of the present study was to determine whether the intensity of bronchiolar epithelial cell injury is ameliorated by transbronchial human IL-10 (hIL-10) gene transfer in a rat model of lung allograft rejection. The left lung was extracted from a donor Brown Norway rat and transbronchially instilled with encoding hIL-10 (IL-10 group) or β-galactosidase (control group). The lung graft was then transplanted into a Lewis rat and harvested on day 6 after transplantation. The allografts were histologically examined and all bronchioles in the slides were assigned one of three grades according to the intensity of epithelial cell loss. The distribution of the grades was significantly different between the two groups, and the epithelial cell injury was significantly improved in the IL-10 group. The present study demonstrated the effect of transbronchial hIL-10 gene transfer on ameliorating bronchiolar epithelial cell injury in a rat model of lung allograft rejection.
This is a preview of subscription content, log in via an institution to check access.
Reference
Christie JD, Edwards LB, Aurora P, Dobbels F, Kirk R, Rahmel AO, et al. The Registry of the International Society for Heart and Lung Transplantation: Twenty-sixth Official Adult Lung and Heart-Lung Transplantation Report — 2009. J Heart Lung Transplant 2009;28:1031–1049.
Orens JB, Estenne M, Arcasoy S, Conte JV, Corris P, Egan JJ, et al. International guidelines for selection of lung transplant candidates: 2006 update — a consensus report from the pulmonary scientific council of the International Society for Heart and Lung Transplantation. J Heart Lung Transplant 2006;25:745–755.
Oishi H, Okada Y, Kikuchi T, Hoshikawa Y, Sado T, Noda M, et al. Transbronchial human interleukin-10 gene transfer reduces acute inflammation associated with allograft rejection and intragraft interleukin-2 and tumor necrosis factor-á gene expression in a rat model of lung transplantation. J Heart Lung Transplant 2010;29:360–367.
Rojas M, Olivier M, Gros P, Barrera LF, García LF. TNF-alpha and IL-10 modulate the induction of apoptosis by virulent Mycobacterium tuberculosis in murine macrophages. J Immunol 1999;162:6122–6131.
Oyaizu T, Okada Y, Shoji W, Matsumura Y, Shimada K, Sado T, et al. Reduction of recipient macrophages by gadolinium chloride prevents development of obliterative airway disease in a rat model of heterotopic tracheal transplantation. Transplantation 2003;76:1214–1220.
Hertz MI, Henke CA, Nakhleh RE, Harmon KR, Marinelli WA, Fox JM, et al. Obliterative bronchiolitis after lung transplantation: a fibroproliferative disorder associated with platelet-derived growth factor. Proc Natl Acad Sci USA 1992;89:10385–10389.
Boehler A, Chamberlain D, Xing Z, Slutsky AS, Jordana M, Gauldie J, et al. Adenovirus-mediated interleukin-10 gene transfer inhibits post-transplant fibrous airway obliteration in an animal model of bronchiolitis obliterans. Hum Gene Ther 1998;9:541–551.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oishi, H., Okada, Y., Kikuchi, T. et al. The intensity of bronchiolar epithelial cell injury caused by an alloimmune response is ameliorated by transbronchial human interleukin-10 gene transfer in a rat model of lung transplantation. Surg Today 41, 1458–1460 (2011). https://doi.org/10.1007/s00595-010-4472-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00595-010-4472-0