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Effect of a prostaglandin I2 analog on the expression of thrombomodulin in liver and spleen endothelial cells after an extensive hepatectomy

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Abstract

Purpose

Dysfunction of the remnant liver after a hepatectomy is caused by microthrombus formation due to endothelial cell (EC) damage. This study evaluated the effect of prostaglandin I2 (PGI2) on the expression of thrombomodulin (TM), a marker for the anticoagulant properties of ECs, using cultured human umbilical vein endothelial cells (HUVECs), and using a canine extensive hepatectomy model.

Methods

The presence of PGI2 receptors was confirmed on HUVECs by reverse transcription-polymerase chain reaction, and the effect of the PGI2 analog on TM expression on HUVECs was determined by an enzyme-linked immunosorbent assay. Twenty mongrel dogs were divided into four groups comprising a sham operation, 70% hepatectomy, 84% hepatectomy, and 84% hepatectomy, with the administration of the PGI2 analog, respectively, and TM expression in the liver, spleen, pancreas, kidney, lung, portal vein, and intestine was determined immunohistochemically.

Results

The TM expression on HUVECs was upregulated by the PGI2 analog. The TM expression on ECs in the hepatic sinusoids and splenic sinus were markedly decreased after the 84% hepatectomy, but such damage was markedly mitigated following an 84% hepatectomy with administration of the PGI2 analog.

Conclusions

An extensive hepatectomy induced severe EC damage not only in the hepatic sinusoids but in the splenic sinuses as well. Prostaglandin I2 prevented damage to these ECs, suggesting that PGI2 improves the microcirculation in the remnant liver.

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Authors and Affiliations

  1. Departments of Hepatobiliary Pancreatic and Transplant Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan

    Masanobu Usui, Hiroyuki Kato, Naohisa Kuriyama, Yoshinori Azumi, Masashi Kishiwada, Shugo Mizuno, Hiroyuki Sakurai, Masami Tabata & Shuji Isaji

  2. Departments of Molecular Pathobiology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan

    Tatsuya Hayashi & Koji Suzuki

Authors
  1. Masanobu Usui
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  2. Hiroyuki Kato
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  9. Tatsuya Hayashi
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  10. Koji Suzuki
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  11. Shuji Isaji
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Usui, M., Kato, H., Kuriyama, N. et al. Effect of a prostaglandin I2 analog on the expression of thrombomodulin in liver and spleen endothelial cells after an extensive hepatectomy. Surg Today 41, 230–236 (2011). https://doi.org/10.1007/s00595-009-4263-7

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  • DOI: https://doi.org/10.1007/s00595-009-4263-7

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