Effect of electrolyzed strong acid water on peritoneal irrigation of experimental perforated peritonitis
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Electrolyzed strong acid water (ESAW) is generated by the electrolysis of a weak sodium chloride solution. Although ESAW is known to have a strong bactericidal activity and to be harmless to the living body, its effectiveness and safety in the treatment of perforated peritonitis has not been well established.
Male Wistar rats were used for the study. Three hours after cecal ligation and puncture, the cecum was resected and the peritoneal cavity was irrigated with 50 ml of saline (Group S, n=12) or ESAW (Group E, n=14). The 5-day survival rate was compared between the two groups. In another pair of animals (n=10 each), bacteria in the ascitic fluid were counted at 6 and 18 h after irrigation.
No adverse effects of ESAW were observed in the experimental group. The 5-day survival rate was 25% (3/12) and 85.7% (12/14) in Groups S and E, respectively (P < 0.01). The bacterial count at 18 h after the irrigation in Groups S and E was (5.0 ± 2.5) × 105/ml and (2.2 ± 2.0) × 104/ml, respectively (P < 0.0001).
Peritoneal lavage with ESAW had no adverse effect, and achieved more effective decontamination than saline for perforated peritonitis. Therefore, the results of this study are considered to warrant and support the clinical application of ESAW.
Key wordsPerforated peritonitis Peritoneal lavage Electrolyzed strong acid water Hypochlorous acid water Bactericidal activity
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- 4.Iwasawa A, Nakamura Y. Bactericidal effect of acidic electrolyzed water-comparison of chemical acidic sodium hydrochloride (NaOCl) solution (in Japanese with English abstract). Kansenshogaku Zashi (J Jpn Assoc Infect Dis) 1996;70:915–922.Google Scholar
- 6.Abe S, Miya Y, Okuda R. Inactivation of hepatitis B virus by high oxidation potential water (in Japanese with English abstract). Nihon Shikahozongaku Zasshi (Jpn J Conserv Dent) 1994;37:1616–1623.Google Scholar
- 12.Kubota A, Hoki M, Yonekura T, Nose K, Hirooka S, Kosumi T, et al. Effectiveness of acid oxidative potential water in peritoneal lavage for perforated appendicitis. Asian J Surg 1999;22:282–283.Google Scholar
- 13.Takemura M, Iwamoto K, Goshi S. intraoperative irrigation with super oxidation water for perforated appendicitis (in Japanese). Geka (Jpn J Surg) 1999;61:1303–1305.Google Scholar
- 14.Ohuchi S, Kawazoe K, Ishihara K, Izumoto H, Eishi K. Management with closed irrigation for post-sternotomy mediastinitis. Jpn J Thorac Cardiovasc Surg 2003;511–514.Google Scholar
- 16.Hotta K. Acid electrolyzed saline solution: its function and medical application. J Jpn Soc Intensive Care Med 2000;7:97–105.Google Scholar
- 17.Iwasawa A, Nakamura Y, Mizuno T. Bactericidal activity of aqua oxidation water against clinically isolated strains (in Japanese). Nihon Kankyo-Kansensyogaku Zasshi (J Jpn Environ Infect) 1993;8:11–16.Google Scholar
- 19.Iwasawa A, Nakamura Y. Cytotoxic effect of antiseptics: comparison In vitro, in vivo examination of strong acidic electrolyzed water, povidone-iodine, chlorhexidine and benzalkonium chloride (in Japanese with English abstract). Kansensyogaku Zasshi (J Jpn Assoc Infect Dis) 2003;77:316–322.Google Scholar
- 21.Otoguro K, Suzuki F, Akimaru Y, Iijima H, Yajima Y, Uebaba K, et al. Hand disinfectant activities of two kinds of electrolysed acid aqueous solutions by globe juice method (in Japanese with English abstract). Nihon Kankyo Kansensyogaku Zasshi (J Jpn Environ Infect) 1996;11:117–122.Google Scholar