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The Effect of Nω-Nitro-l-Arginine Methyl Ester and l-Arginine on Lung Injury Induced by Abdominal Aortic Occlusion–Reperfusion

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Abstract

Purpose

The aim of this study was to examine the effects of Nω-nitro-l-arginine methyl ester (l-NAME) and l-arginine on lung injury after aortic ischemia–reperfusion (IR).

Methods

Twenty-four Wistar-Albino rats were randomized into four groups (n = 6) as follows: Control (sham laparotomy), Aortic IR (30 min ischemia and 120 min reperfusion), l-Arginine (intraperitoneal 100 mg kg<συπ>−1<?συπ> live weight)+aortic IR, and l-NAME (intraperitoneal 10 mg kg<συπ>−1<?συπ> live weight)+aortic IR. In the lung specimens, the tissue levels of malondialdehyde (MDA), vascular endothelial growth factor (VEGF), and nitric oxide (NO) were measured and a histological examination was done.

Results

Aortic IR increased MDA, VEGF, and NO. l-Arginine further significantly increased MDA and NO, and decreased VEGF (P < 0.05 vs aortic IR). l-NAME significantly decreased MDA and NO (P < 0.05 vs l-arginine+aortic IR) and increased VEGF (P < 0.05 vs other groups). A histological examination showed the aortic IR to significantly increase (P < 0.05 vs control) while l-arginine also further increased (P > 0.05 vs aortic IR), whereas l-NAME caused a significant decrease in pulmonary leukocyte infiltration (P < 0.05 vs aortic IR).

Conclusions

Our results indicate that l-arginine aggravates the lung injury induced by aortic IR, while l-NAME attenuates it.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Suleyman Demirel University Medical School, Isparta, Turkey

    Huseyin Okutan & Ilker Kiris

  2. Department of Medical Microbiology, Suleyman Demirel University Medical School, Isparta, Turkey

    Ali K. Adiloglu

  3. Department of Pediatric Surgery, Suleyman Demirel University Medical School, Isparta, Turkey

    Cagri Savas

  4. Department of Pathology, Suleyman Demirel University Medical School, Isparta, Turkey

    Nilgün Kapucuoğlu

  5. Department of Biochemistry, Suleyman Demirel University Medical School, Isparta, Turkey

    Irfan Altuntas & Onur Akturk

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  1. Huseyin Okutan
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Okutan, H., Kiris, I., Adiloglu, A. et al. The Effect of Nω-Nitro-l-Arginine Methyl Ester and l-Arginine on Lung Injury Induced by Abdominal Aortic Occlusion–Reperfusion. Surg Today 38, 30–37 (2008). https://doi.org/10.1007/s00595-007-3575-8

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