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The Effect of Purified Micronized Flavonoid Fraction on the Healing of Anastomoses in the Colon in Rats

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Abstract

Purpose

Anastomotic leakage of colonic and rectal anastomoses is a major complication after large intestine surgery. Many factors influence the healing of colon anastomoses. Flavonoids have been recognized for centuries as physiologically active constituents that are used to treat human diseases. We studied the effects of a clinically used, micronized, purified flavonoid fraction on the healing of colonic anastomosis in rats.

Methods

Male Sprague–Dawley rats were used. The flavonoid group of rats received 100 mg/kg per day of Daflon for 14 days until surgery. Thereafter, a resection and anastomosis were performed. The bursting pressure of the anastomoses and the hydroxyproline levels of the perianastomotic tissue were determined to evaluate the healing on the third and seventh days of surgery for both flavonoid and control groups.

Results

The bursting pressure of the flavonoid group was higher on the seventh day. The hydroxyproline levels of the flavonoid group were significantly higher than in the control group on both the third and seventh days after surgery.

Conclusions

Although the micronized purified flavonoid fraction has some inhibitory properties on the healing of the anastomosis, its net effect was to obtain a better anastomotic healing of the colon in rats.

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References

  1. T Kiyama M Onda A Tokunaga DT Efron A Barbul (2001) ArticleTitleEffect of matrix metalloproteinase inhibition on colonic anastomotic healing in rats J Gastrointest Surg 5 303–11 Occurrence Handle11360054 Occurrence Handle1:STN:280:DC%2BD3MzjtlCkuw%3D%3D Occurrence Handle10.1016/S1091-255X(01)80052-4

    Article  PubMed  CAS  Google Scholar 

  2. P Chintra GB Sajithlal G Chandrakasan (1998) ArticleTitleInfluence of aloe vera on the healing of dermal wounds in diabetic rats J Ethnopharmacol 59 195–201 Occurrence Handle10.1016/S0378-8741(97)00124-4

    Article  Google Scholar 

  3. W Vaneerdeweg JMH Hendriks PRM Lauwers M Ieven EJM Eyskens (2000) ArticleTitleEffect of gentamicin-containing sponges on the healing of colonic anastomoses in a rat model of peritonitis Eur J Surg 166 959–62 Occurrence Handle11152258 Occurrence Handle1:CAS:528:DC%2BD3MXhtlGgtw%3D%3D Occurrence Handle10.1080/110241500447137

    Article  PubMed  CAS  Google Scholar 

  4. MHJ Verhofstad WP Lange JAWM Van der Laak AAJ Verhofstad T Hendricks (2001) ArticleTitleMicroscopic analysis of anastomotic healing in the intestine of normal and diabetic rats Dis Colon Rectum 44 423–31 Occurrence Handle11289291 Occurrence Handle1:STN:280:DC%2BD3M7pvFyqsw%3D%3D Occurrence Handle10.1007/BF02234744

    Article  PubMed  CAS  Google Scholar 

  5. P Månsson XW Zhang B Jeppsson H Thorlacius (2002) ArticleTitleAnastomotic healing in the rat colon: comparison between a radiological method, breaking strength and bursting pressure Int J Colorectal Dis 17 420–5 Occurrence Handle12355219 Occurrence Handle10.1007/s00384-002-0392-9

    Article  PubMed  Google Scholar 

  6. AC Van der Ham WJ Kort AM Bijma FJ Zijlstra MA Wermeer J Jeekel (1990) ArticleTitleEicosanoid profile of healing colon anastomosis and peritoneal macrophages in the rat Gut 31 807–11 Occurrence Handle2370016 Occurrence Handle1:CAS:528:DyaK3MXnvFGhug%3D%3D

    PubMed  CAS  Google Scholar 

  7. RL Cali TC Smyrk GJ Blatchford AG Thorson MA Christensen (1993) ArticleTitleEffect of prostaglandin E1 and steroid on healing colonic anastomosis Dis Colon Rectum 36 1148–51 Occurrence Handle8253012 Occurrence Handle1:STN:280:ByuD2svks10%3D Occurrence Handle10.1007/BF02052264

    Article  PubMed  CAS  Google Scholar 

  8. B Egger R Inglin J Zeeh O Dirsch Y Huang MW Büchler (2001) ArticleTitleInsulin-like growth factor I and truncated keratinocyte growth factor accelarate healing of left sided colonic anastomoses Br J Surg 88 90–8 Occurrence Handle11136318 Occurrence Handle1:STN:280:DC%2BD3M7isFGmsA%3D%3D Occurrence Handle10.1046/j.1365-2168.2001.01617.x

    Article  PubMed  CAS  Google Scholar 

  9. TL Buchmiller-Crair CS Kim NH Won HL Chopourian KS Shaw EW Fonkalsrud (2001) ArticleTitleEffect of acute anemia on the healing of intestinal anastomoses in the rabbit J Trauma 51 363–8 Occurrence Handle11493801 Occurrence Handle1:STN:280:DC%2BD3Mvks1Kqsw%3D%3D

    PubMed  CAS  Google Scholar 

  10. D Nolte S Pickelmann M Möllmann et al. (1999) ArticleTitleEffects of the phlebotropic drug Daflon 500 mg on postischemic microvascular disturbances in striated skin muscle: an intravital microscopic study in the hamster J Lab Clin Med 134 526–35 Occurrence Handle10560947 Occurrence Handle1:CAS:528:DyaK1MXnvVejtrg%3D Occurrence Handle10.1016/S0022-2143(99)90175-0

    Article  PubMed  CAS  Google Scholar 

  11. C Labrid (1994) ArticleTitlePharmacologic properties of Daflon 500 mg Angiology 45 524–30 Occurrence Handle8203782 Occurrence Handle1:STN:280:ByuB28zgsl0%3D

    PubMed  CAS  Google Scholar 

  12. IS Jamall VN Finelli SS Que Hee (1981) ArticleTitleA simple method to determine nanogram levels of 4-hydroxyproline in biological tissues Anal Biochem 112 70–5 Occurrence Handle7258630 Occurrence Handle1:CAS:528:DyaL3MXhslSnuro%3D Occurrence Handle10.1016/0003-2697(81)90261-X

    Article  PubMed  CAS  Google Scholar 

  13. RSR Zand DJA Jenkins EP Diamandis (2002) ArticleTitleFlavonoids and steroid hormone-dependent cancers J Chromatogr B Anal Technol Biomed Life Sci 777 219–32 Occurrence Handle1:CAS:528:DC%2BD38Xos1WrtbY%3D

    CAS  Google Scholar 

  14. T Jean MC Bodinier (1994) ArticleTitleMediators involved in inflammation: effects of Daflon 500 mg on their release Angiology 45 554–9 Occurrence Handle8203787 Occurrence Handle1:STN:280:ByuB28zgvVA%3D

    PubMed  CAS  Google Scholar 

  15. M Lonchampt B Guardiola N Sicot M Bertrand L Perdrix J Duhault (1989) ArticleTitleProtective effect of a purified flavonoid fraction against reactive oxygen radicals Drug Res 39 882–5 Occurrence Handle1:CAS:528:DyaL1MXlt1KltrY%3D

    CAS  Google Scholar 

  16. M Salucci LA Stivala G Maiani R Bugianesi V Vannini (2002) ArticleTitleFlavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells Br J Canncer 86 1645–51 Occurrence Handle1:CAS:528:DC%2BD38XkvVaitbY%3D Occurrence Handle10.1038/sj.bjc.6600295

    Article  CAS  Google Scholar 

  17. FZGA Cyrino DA Bottino L Lerond E Bouskela (2004) ArticleTitleMicronization enhances the protective effect of purified flavonoid fraction against postischaemic microvascular injury in the hamster cheek pouch Clin Exp Pharmacol Physiol 31 159–62 Occurrence Handle15008958 Occurrence Handle1:CAS:528:DC%2BD2cXitlWntbY%3D Occurrence Handle10.1111/j.1440-1681.2004.03974.x

    Article  PubMed  CAS  Google Scholar 

  18. E Bouskela FZGA Cyrino L Lerond (1999) ArticleTitleLeukocyte adhesion after oxidant challenge in the hamster cheek pouch microcirculation J Vasc Res 36 IssueIDsuppl 1 S11–4 Occurrence Handle10.1159/000054069

    Article  Google Scholar 

  19. PDC Smith (1999) ArticleTitleNeutrophil activation and mediators of inflammation in chronic venous insufficiency J Vasc Res 36 IssueIDsuppl 1 S24–36 Occurrence Handle10.1159/000054071

    Article  Google Scholar 

  20. RJ Korthuis DC Gute (1997) ArticleTitlePostischemic leukocyte/endothelial cell interactions and microvascular barrier dysfunction in skeletal muscle: cellular mechanisms and effect of Daflon 500 mg Int J Microcirc 17 IssueIDsuppl 1 11–7 Occurrence Handle1:CAS:528:DyaK1cXhtlSmtLs%3D

    CAS  Google Scholar 

  21. S Pickelmann D Nolte R Leiderer M Möllmann E Schütze K Messmer (1999) ArticleTitleEffects of the phlebotropic drug Daflon 500 mg on postischemic reperfusion injury in striated skin muscle: a histomorphologic study in the hamster J Lab Clin Med 134 536–45 Occurrence Handle10560948 Occurrence Handle1:CAS:528:DyaK1MXnvVejtrk%3D Occurrence Handle10.1016/S0022-2143(99)90176-2

    Article  PubMed  CAS  Google Scholar 

  22. M Cömert F Taneri E Tekin et al. (2000) ArticleTitleThe effect of pentoxifylline on the healing of intestinal anastomosis in rats with experimental obstructive jaundice Surg Today 30 896–902 Occurrence Handle11059729 Occurrence Handle10.1007/s005950070041

    Article  PubMed  Google Scholar 

  23. T Hendricks WJB Mastboom (1990) ArticleTitleHealing of experimental intestinal anastomoses Dis Colon Rectum 33 891–901 Occurrence Handle10.1007/BF02051930

    Article  Google Scholar 

  24. M Seyer-Hansen TT Andreassen H Cristensen H Oxlund (1999) ArticleTitleEffect of experimental diabetes and growth hormone administration on the strength of colonic anastomoses in rats Eur Surg Res 31 419–28 Occurrence Handle10529556 Occurrence Handle1:CAS:528:DyaK1MXmvFKru78%3D Occurrence Handle10.1159/000008721

    Article  PubMed  CAS  Google Scholar 

  25. L Ugurlu M Turan E Canbay S Elagoz M Sen (2003) ArticleTitleEffect of nifedipine on the healing of left colonic anastomoses in rats Surg Today 33 902–8 Occurrence Handle14669080 Occurrence Handle1:CAS:528:DC%2BD3sXps1yhtLk%3D Occurrence Handle10.1007/s00595-003-2633-0

    Article  PubMed  CAS  Google Scholar 

  26. BA Mast (1997) ArticleTitleHealing in other tissues Surg Clin N Am 77 529–47 Occurrence Handle9194879 Occurrence Handle1:STN:280:ByiA3sfjtF0%3D Occurrence Handle10.1016/S0039-6109(05)70567-3

    Article  PubMed  CAS  Google Scholar 

  27. P Chithra GB Sajithlal G Chandrakasan (1998) ArticleTitleInfluence of Aloe vera on collagen characteristics in healing dermal wounds in rats Mol Cell Biochem 181 71–6 Occurrence Handle9562243 Occurrence Handle1:CAS:528:DyaK1cXit1ait7g%3D Occurrence Handle10.1023/A:1006813510959

    Article  PubMed  CAS  Google Scholar 

  28. FJ Thornton A Barbul (1997) ArticleTitleHealing in the gastrointestinal tract Surg Clin N Am 77 549–73 Occurrence Handle9194880 Occurrence Handle1:STN:280:ByiA3sfjt1Q%3D Occurrence Handle10.1016/S0039-6109(05)70568-5

    Article  PubMed  CAS  Google Scholar 

  29. T Terzioglu YE Sonmez U Eldegez (1990) ArticleTitleThe effect of prostaglandin E1 on colonic anastomotic healing Dis Colon Rectum 33 44–8 Occurrence Handle1688525 Occurrence Handle1:STN:280:By%2BC3cjmtlQ%3D Occurrence Handle10.1007/BF02053201

    Article  PubMed  CAS  Google Scholar 

  30. AA Ramelet (2001) ArticleTitleClinical benefits of Daflon 500 mg in the most severe stages of stages of chronic venous insufficiency Angiology 52 IssueIDsuppl 1 S49–56 Occurrence Handle11510597

    PubMed  Google Scholar 

  31. BK Ener (2000) ArticleTitleDorsal pedal venous oximetry for assessing treatment of chronic venous insufficiency: effects of Daflon 500 mg Vasc Surg 34 43–9

    Google Scholar 

  32. M Damon O Flandre F Michel L Perdrix C Labrid A Crastes de Paulet (1987) ArticleTitleEffect of chronic treatment with a purified flavonoid fraction on inflammatory granuloma in the rat. Study of prostaglandin E2 and F2 alpha and thromboxane B2 release and histological changes Arzneim-Forsch/Drug Res 37 1149–53 Occurrence Handle1:CAS:528:DyaL1cXjtlE%3D

    CAS  Google Scholar 

  33. RJ Korthuis DC Gute (1999) ArticleTitleAdhesion molecule expression in postischemic microvascular dysfunction: activity of a micronized purified flavonoid fraction J Vasc Res 36 IssueIDsuppl 1 S15–23 Occurrence Handle10.1159/000054070

    Article  Google Scholar 

  34. L McGregor M Bellangeon E Chignier L Lerond C Rousselle JL McGregor (1999) ArticleTitleEffect of a micronized purified flavonoid fraction on in vivo platelet functions in the rat Thromb Res 94 235–40 Occurrence Handle10336239 Occurrence Handle1:CAS:528:DyaK1MXjtVKktLc%3D Occurrence Handle10.1016/S0049-3848(98)00216-3

    Article  PubMed  CAS  Google Scholar 

  35. PDC Smith (2003) ArticleTitleFrom skin disorders to venous leg ulcers: pathophysiologiy and efficacy of Daflon 500 mg in ulcer healing Angiology 54 IssueIDsuppl 1 S45–50 Occurrence Handle10.1177/000331970305400106

    Article  Google Scholar 

  36. RJ Korthuis DC Gute (2002) ArticleTitleAnti-inflammatory actions of a micronized, purified flavonoid fraction in ischemia/reperfusion Adv Exp Med Biol 505 181–90 Occurrence Handle1:CAS:528:DC%2BD38Xms1agsr8%3D

    CAS  Google Scholar 

  37. E Bouskela KA Donyo (1997) ArticleTitleEffects of oral administration of purified micronized flavonoid fraction on increased microvascular permeability induced by various agents and on ischemia/reperfusion in the hamster cheek pouch Angiology 48 391–9 Occurrence Handle9158383 Occurrence Handle1:STN:280:ByiA3c3gt1Y%3D Occurrence Handle10.1177/000331979704800503

    Article  PubMed  CAS  Google Scholar 

  38. CN Rao VH Rao B Steinmann (1981) ArticleTitleInfluence of bioflavonoids on the collagen metabolism in rats with adjuvant induced arthritis Ital J Biochem 30 54–62 Occurrence Handle7251341 Occurrence Handle1:CAS:528:DyaL3MXkslentrs%3D

    PubMed  CAS  Google Scholar 

  39. CN Rao VH Rao B Steinmann (1981) ArticleTitleInfluence of bioflavonoids on the metabolism and crosslinking of collagen Ital J Biochem 30 259–70 Occurrence Handle7298310 Occurrence Handle1:CAS:528:DyaL38XivVeqsg%3D%3D

    PubMed  CAS  Google Scholar 

  40. M Salucci LA Stivala G Maiani R Bugianesi V Vannini (2002) ArticleTitleFlavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells Br J Cancer 86 1645–51 Occurrence Handle12085217 Occurrence Handle1:CAS:528:DC%2BD38XkvVaitbY%3D Occurrence Handle10.1038/sj.bjc.6600295

    Article  PubMed  CAS  Google Scholar 

  41. MB Witte A Barbul (1997) ArticleTitleGeneral principles of wound healing Surg Clin N Am 77 509–28 Occurrence Handle9194878 Occurrence Handle1:STN:280:ByiA3sfjtFw%3D Occurrence Handle10.1016/S0039-6109(05)70566-1

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery, Fatih University School of Medicine, Beştepe, Emek 06510, Ankara, Türkiye

    Aydin Ïnan, Meral Şen, Cemile Koca, Ayhan Akpinar & Cenap Dener

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  1. Aydin Ïnan
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  2. Meral Şen
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  3. Cemile Koca
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Ïnan, A., Şen, M., Koca, C. et al. The Effect of Purified Micronized Flavonoid Fraction on the Healing of Anastomoses in the Colon in Rats. Surg Today 36, 818–822 (2006). https://doi.org/10.1007/s00595-006-3251-4

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