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The Protective Effect of α-Tocopherol and GdCl3 Against Hepatic Ischemia/Reperfusion Injury

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Abstract

Purpose

To evaluate the combined effect of α-tocopherol and gadolinium chloride (GdCl3) in reducing lipid peroxidation after severe hepatic ischemia/reperfusion (IR) injury.

Methods

Sixty male Wistar rats, 200–250 g, were randomly divided into six equal groups. There were two sham operation (SHAM) groups, two untreated IR groups, and two IR groups treated with GdCl3 and α-tocopherol (IRGT). After 60 min of total hepatic ischemia and 120 min reperfusion, one of each group was killed, liver samples were taken for malondialdehyde (MDA) and myeloperoxidase (MPO) analysis and light microscopy examination, and blood samples were analyzed for aspartate (AST) and alanine (ALT) transaminase, lactate dehydrogenase (LDH), and α-tocopherol content. The remaining groups were monitored for survival rate determination.

Results

The mean MDA and MPO values in the SHAM, IR, and IRGT groups, respectively, were 1.117, 1.476, and 0.978 nmol/g wet tissue and 1.49, 6.26, and 1.78 (U/g). The mean α-tocopherol values in the SHAM, IR, and IRGT groups, respectively, were 10.4, 1.9, and 12 µmol/l. The mean serum AST, ALT, and LDH values were significantly higher in the IR group than in the SHAM group (P < 0.001), and significantly lower in the IRGT group than in the IR group (P < 0.001). Light microscopy examination revealed more severe congestion and vacuolization in the IR group than in the SHAM group, and minimal congestion and vacuolization in the IRGT group. Survival was significantly higher in the IRGT group than in the IR group.

Conclusion

The administration of GdCl3 and α-tocopherol is likely to protect the liver against lipid peroxidation by suppressing Kupffer cell and polymorphonuclear leukocyte activation and enhancing endogenous antioxidant activity.

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Authors and Affiliations

  1. Department of Transplantation Surgery, Medical School, Aristotle University, Hippokration Hospital, Konstantinoupoleos 49, 54642, Thessaloniki, Greece

    Dimitrios Giakoustidis, Alexandros Giakoustidis, Nickolas Kontos, Dimitrios Tsantilas, Vasilios Papanikolaou & Dimitrios Takoudas

  2. Department of Biological Chemistry, Medical School, Aristotle University, Hippokration Hospital, Konstantinoupoleos 49, 54642, Thessaloniki, Greece

    George Papageorgiou, Stavros Iliadis & Evropi Botsoglou

  3. Department of Pathology and Histology, Medical School, Aristotle University, Hippokration Hospital, Konstantinoupoleos 49, 54642, Thessaloniki, Greece

    Evanthia Kostopoulou

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  1. Dimitrios Giakoustidis
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  2. George Papageorgiou
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Giakoustidis, D., Papageorgiou, G., Iliadis, S. et al. The Protective Effect of α-Tocopherol and GdCl3 Against Hepatic Ischemia/Reperfusion Injury. Surg Today 36, 450–456 (2006). https://doi.org/10.1007/s00595-005-3162-9

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