Abstract
We evaluated the ability of zinc to modulate the superoxide anion release by isolated, phorbol myristate acetate (PMA)-stimulated polymorphonuclear leukocytes (PMNs). In the presence of zinc at physiological level (20 μmol/l). PMNs released 20% less superoxide anions than untreated cells, whereas at higher zinc concentration (200 μmol/l), a 40%-increase in superoxide anion production was detected. The direction (suppression or stimulation) of superoxide anion generation was dependent upon extracellular zinc level and did not discriminate type 1 and 2 diabetic subjects from normal controls. The mechanism(s) of dose-dependent pro- and antioxidative effects of zinc on isolated PMNs in vitro as well as the implication of this finding for the modulatory effect of Zn on PMN-superoxide anion production in vivo need further investigation.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 28 March 2000 / Accepted in revised form: 20 September 2000
Rights and permissions
About this article
Cite this article
Gavella, M., Lipovac, V. & Car, A. In vitro effect of zinc on superoxide anion production by polymorphonuclear leukocytes of diabetic patients. Acta Diabetol 37, 135–137 (2000). https://doi.org/10.1007/s005920070016
Issue Date:
DOI: https://doi.org/10.1007/s005920070016