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The HIF1α polymorphism rs2301104 is associated with obesity and obesity-related cytokines in Han Chinese population

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Abstract

Aims

To evaluate the association between genetic polymorphisms of HIF1α and obesity and obesity-related cytokines in the Han Chinese population.

Methods

The study consisted of 160 subjects with obesity and 166 age- and gender-matched healthy controls. We genotyped three tag single nucleotide polymorphisms (SNPs) of HIF1α by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based genotyping technology. Plasma cytokine concentrations were determined on the Luminex platform. The genetic associations were analysed statistically.

Results

Obese subjects had significant obesity-related metabolic abnormalities, including hyperglycaemia, insulin resistance, and abnormalities in blood lipids, liver enzymes, and uric acid levels. SNP analysis of HIF1α revealed that the allele and genotype frequencies of rs2301104 were significantly associated with obesity. Our results suggest that the minor allele C of rs2301104 might be a protective mutation of obesity, and CC/CG genotypes of rs2301104 could be protective genotype of obesity. We also found that subjects with CC/CG genotypes of rs2301104 had significantly lower levels of IL-6, TNF-α, IL-1β, IL-8, and IL-10 than subjects with GG genotypes.

Conclusions

This is the first study to report an association between HIF1α polymorphisms and obesity and obesity-related cytokines in a Han Chinese population. These results require replication in larger populations but suggest that HIF1α may play an important role in obesity development.

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Funding

This project was supported by the National Natural Science Foundation of China Youth Program (Grant No. 81900733), the Chongqing municipal health and Family Planning Commission Fund (Number: 2021MSXM013), Bethune Charity Foundation (G-X-2020–1107-14), and the National Key Clinical Specialties Construction Program of China in 2011.

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Authors and Affiliations

  1. Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, No.1 Friendship Road, Yuzhong District, Chongqing, 400016, China

    Xiaoya Zheng, Jiani Ma, Jian Long & Wei Ren

  2. Prevention of Disease Department, Chongqing Jiulongpo District Hospital of Traditional Chinese Medicine, Chongqing, China

    Qiang Wei

Authors
  1. Xiaoya Zheng
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  2. Jiani Ma
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  3. Jian Long
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  4. Qiang Wei
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  5. Wei Ren
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Corresponding author

Correspondence to Xiaoya Zheng.

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Conflict of interest

All authors declare no conflicts of interest.

Ethical approval

The study was approved by the Human Research Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. The ethics review batch code is 2021–608.

Informed consent

Informed consent was obtained from all individual participants included in the study. Clinical data and peripheral blood samples were collected only after explaining the objectives of the study and obtaining a signed informed consent form.

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Communicated by Fabrizio Barbetti.

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Zheng, X., Ma, J., Long, J. et al. The HIF1α polymorphism rs2301104 is associated with obesity and obesity-related cytokines in Han Chinese population. Acta Diabetol 60, 285–292 (2023). https://doi.org/10.1007/s00592-022-02008-5

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  • DOI: https://doi.org/10.1007/s00592-022-02008-5

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