Acta Diabetologica

, Volume 53, Issue 4, pp 609–619 | Cite as

Protective and hypoglycemic effects of celery seed on streptozotocin-induced diabetic rats: experimental and histopathological evaluation

  • Faezeh Tashakori-Sabzevar
  • Masoud Ramezani
  • Hossein Hosseinzadeh
  • Seyyed Mohammad Reza Parizadeh
  • Ahmad Reza Movassaghi
  • Ahmad Ghorbani
  • Seyed Ahmad MohajeriEmail author
Original Article



Diabetes mellitus is a major manifestation of metabolic disorder which presents with hyperglycemia (high levels of serum blood sugar). In the present study, we aimed to investigate the effects of celery seed extract on different biochemical factors and histopathological changes in normal and streptozotocin (STZ)-induced diabetic rats.


A total of 35 male Wistar rats were divided into five groups (one normal and four diabetic groups). STZ was injected intraperitoneally to induce diabetes. The effects of hexane extract of celery seed and glibenclamide (as a positive control) were compared. Blood samples were analyzed on days 0, 18, and 33, and histopathological evaluations were performed at the end of the study.


Glucose, triglycerides, and cholesterol levels significantly decreased, whereas insulin and high-density lipoprotein (HDL) levels increased in the extract-administered groups, as compared to the negative diabetic control group (P < 0.0001). The concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of the extract-administered groups were significantly less than the negative control group (P < 0.0001). Histopathological reports revealed significantly less atrophy, necrosis, and inflammation in the rats receiving celery seed extract compared to the negative control group.


The results indicated that celery seed extract can be effective in controlling hyperglycemia and hyperlipidemia in diabetic rats, and demonstrated its protective effects against pancreatic toxicity resulting from STZ-induction.


Celery seed Diabetes mellitus Histopathology Rat Streptozotocin 



We gratefully thank the vice chancellor of research, Mashhad University of Medical Sciences, for financial and logistic support of this Project through Grant Number 910889. This result was obtained from a Pharm. D. thesis (Dr. Masoud Ramezani).

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

This article does not contain any study with human participants. All procedures performed in this study involving animals were in accordance with the ethical standards of “Ethics Committee Acts” of Mashhad University of Medical Sciences.

Human and animal rights

This article does not contain any studies with human performed by the any of the authors. The experimental procedures for all animals were in accordance with the Ethics Committee Acts of Mashhad University of Medical Sciences.

Informed consent

For this type of study formal consent is not required.


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Copyright information

© Springer-Verlag Italia 2016

Authors and Affiliations

  • Faezeh Tashakori-Sabzevar
    • 1
  • Masoud Ramezani
    • 1
  • Hossein Hosseinzadeh
    • 2
  • Seyyed Mohammad Reza Parizadeh
    • 3
  • Ahmad Reza Movassaghi
    • 4
  • Ahmad Ghorbani
    • 5
  • Seyed Ahmad Mohajeri
    • 2
    Email author
  1. 1.Student Research Committee (SRC)Mashhad University of Medical SciencesMashhadIran
  2. 2.Pharmaceutical Research Center, School of PharmacyMashhad University of Medical SciencesMashhadIran
  3. 3.Biochemistry of Nutrition Research Center, Faculty of MedicineMashhad University of Medical SciencesMashhadIran
  4. 4.Department of Pathobiology, Faculty of Veterinary MedicineFerdowsi University of MashhadMashhadIran
  5. 5.Pharmacological Research Center of Medicinal Plants, School of MedicineMashhad University of Medical SciencesMashhadIran

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