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Erythrocyte glutathione transferase activity: a possible early biomarker for blood toxicity in uremic diabetic patients

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Abstract

Erythrocyte glutathione transferase (e-GST) displays increased activity in patients with renal damage and positive correlation with homocysteine (Hcy) in patients under maintenance hemodialysis. Here, we determined e-GST, Hcy, and erythrocyte catalase (e-CAT) in 328 patients affected by type 2 diabetes mellitus (T2DM), 61 diabetic non-nephropathic patients and 267 affected by diabetes and by chronic kidney disease (CKD) under conservative therapy subdivided into four stages according to K-DOQI lines. e-GST activity was significantly higher in all T2DM patients compared to the control group (7.90 ± 0.26 vs. 5.6 ± 0.4 U/gHb), and we observed an enhanced activity in all subgroups of CKD diabetic patients. No significant correlation or increase has been found for e-CAT in all patients tested. Mean Hcy in diabetic patients is higher than that in healthy subjects (33.42 ± 1.23 vs. 13.6 ± 0.8 μM), and Hcy increases in relation to the CKD stage. As expected, a significant correlation was found between e-GST and Hcy levels. These findings suggest that e-GST hyperactivity is not caused directly by diabetes but by its consequent renal damage. e-GST, as well as Hcy, may represent an early biomarker of renal failure.

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Acknowledgments

We thank Dr. Olga Durante, Dr. Elisabetta Catena and Dr. Maria Paola Canale for technical assistance. Present study is partially supported by a Grant of Fondazione Roma—Terzo Settore assigned to Prof. G. Ricci.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Nephrology and Hypertension Unit, Department of System Medicine, University of Rome “Tor Vergata”, Viale Oxford 81, 00133, Rome, Italy

    Annalisa Noce, Silvia Santini, Valentina Rovella, Manfredi Tesauro & Nicola Di Daniele

  2. Department of Chemical Sciences and Technologies, University of Rome “Tor Vergata”, Rome, Italy

    Raffaele Fabrini, Alessio Bocedi & Giorgio Ricci

  3. Department of Experimental Medicine and Surgery, University of Rome “Tor Vergata”, Rome, Italy

    Mariarita Dessì, Alessio Bocedi & Sergio Bernardini

  4. Biochemistry Laboratory, Children’s Hospital and Research Institute “Bambino Gesù”, Rome, Italy

    Anna Pastore

Authors
  1. Annalisa Noce
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  2. Raffaele Fabrini
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  3. Mariarita Dessì
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  4. Alessio Bocedi
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  5. Silvia Santini
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  6. Valentina Rovella
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  7. Anna Pastore
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  8. Manfredi Tesauro
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  9. Sergio Bernardini
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  10. Nicola Di Daniele
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  11. Giorgio Ricci
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Corresponding author

Correspondence to Nicola Di Daniele.

Additional information

Communicated by Massimo Federici.

Annalisa Noce, Raffaele Fabrini and Mariarita Dessì have contributed equally to this work.

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Noce, A., Fabrini, R., Dessì, M. et al. Erythrocyte glutathione transferase activity: a possible early biomarker for blood toxicity in uremic diabetic patients. Acta Diabetol 51, 219–224 (2014). https://doi.org/10.1007/s00592-013-0497-3

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  • DOI: https://doi.org/10.1007/s00592-013-0497-3

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