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Allogeneic umbilical cord mesenchymal stem cell conditioned medium (secretome) for treating posterior cruciate ligament rupture: a prospective single-arm study

European Journal of Orthopaedic Surgery & Traumatology volume 33, pages 669–675 (2023)Cite this article

Abstract

Background

Outcomes of the current management of posterior cruciate ligament (PCL) rupture are still unsatisfactory. Recent literature demonstrated the efficacy of the paracrine action of mesenchymal stem cells (MSC) in ligament rupture healing. This study aimed to evaluate the outcome of arthroscopic administration of allogeneic umbilical cord-derived MSC (UC-MSC) conditioned medium (secretome) for the treatment of PCL rupture.

Patients and methods

This is a prospective study including 12 individuals with PCL rupture grade 1 or 2 who were performed arthroscopy and secretome administrations. The functional and radiologic outcome of the knee was examined one year following intervention.

Results

Preoperatively, posterior drawer test revealed three cases of grade 2+ and nine cases of grade 1+, whereas the final follow-up revealed two cases of grade 2+ and ten cases of grade 1+ PCL rupture. At final follow-up, the mean scores for the IKDC, modified Cincinnati, and Lysholm were 90.58 ± 4.30, 90.90 ± 2.15, and 89.42 ± 3.16, respectively. The means of the serial hop tests were 90.33, 94.16, 93.66, and 95.33 for single, triple, crossover, and time hop tests, respectively. Five patients were able to resume competitive sport after an average of 25.8 weeks (25–38). The final MRI analysis revealed that six knees (50%) regained PCL continuity with low signal intensity, five knees (41.6%) returned near-normal PCL continuity, and one knee (8.3%) regained PCL continuity but with deformed outlines.

Conclusions

Short-term follow-up indicated that the secretome generated from allogenic UC-MSC produces excellent functional and radiographic results in grade I-II PCL rupture.

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Acknowledgements

We would like to express our gratitude to the staff of Klinik Bahasa FKKMK UGM for their assistance in preparing this manuscript.

Funding

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Authors and Affiliations

  1. Sport and Adult Reconstruction Division, Department of Orthopaedic and Traumatology, Soeradji Tirtonegoro Hospital, Klaten, Indonesia

    Sholahuddin Rhatomy

  2. Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Sholahuddin Rhatomy

  3. Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

    Jeanne Adiwinata Pawitan

  4. Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

    Jeanne Adiwinata Pawitan & Ismail Hadisoebroto Dilogo

  5. Stem Cell and Tissue Engineering Research Cluster Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jl. Salemba Raya No. 5, Jakarta, Indonesia

    Jeanne Adiwinata Pawitan

  6. Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, Jl. Diponegoro no. 5, Jakarta, Indonesia

    Trie Kurniawati

  7. Orthopaedic Surgeon of Jakarta Knee, Shoulder and Orthopaedic Sport Clinic, Pondok Indah General Hospital, Jl. Metro Duta Kav UE, Jakarta Selatan, 12310, Indonesia

    Jessica Fiolin

  8. Orthopaedic and Traumatology Department, Faculty of Medicine, RS DR Cipto Mangunkusumo, Jakarta, Indonesia

    Ismail Hadisoebroto Dilogo

  9. Stem Cell and Tissue Engineering Research Cluster Indonesian Medical Education and Research Institute (IMERI), Universitas Indonesia, Jl. Diponegoro no 71, Jakarta, Indonesia

    Ismail Hadisoebroto Dilogo

Authors
  1. Sholahuddin Rhatomy
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  2. Jeanne Adiwinata Pawitan
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  3. Trie Kurniawati
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  4. Jessica Fiolin
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  5. Ismail Hadisoebroto Dilogo
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Correspondence to Sholahuddin Rhatomy.

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Ethical approval

This study had been reviewed by our institutional review board and received ethical clearance number KE-1113/EC/2019.

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The authors declare that they have no competing interests.

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Cite this article

Rhatomy, S., Pawitan, J.A., Kurniawati, T. et al. Allogeneic umbilical cord mesenchymal stem cell conditioned medium (secretome) for treating posterior cruciate ligament rupture: a prospective single-arm study. Eur J Orthop Surg Traumatol 33, 669–675 (2023). https://doi.org/10.1007/s00590-022-03278-z

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  • DOI: https://doi.org/10.1007/s00590-022-03278-z

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