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Enhanced nerve autograft using stromal vascular fraction

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European Journal of Orthopaedic Surgery & Traumatology Aims and scope Submit manuscript

Abstract

Purpose

While many studies have been conducted on peripheral nerve regeneration, few have focused on strengthening the nerve autografts. This study hypothesized that adding autologous stromal vascular fraction (SVF) to a nerve autograft will improve nerve regeneration. The purpose of this study was to compare the results of nerve autograft with and without SVF.

Methods

An adipose tissue sample was excised from the right inguinal region of female Wistar rats, and SVF was separated by centrifugation. The left sciatic nerve was resected at a length of 15 mm and the defect was bridged by a resected nerve autograft. We added SVF with collagen gel around the nerve autograft in the SVF group and added saline in the control group. At 12 weeks after surgery, the wet muscle weight, distal latency, and amplitude of the compound muscle action potential of the tibialis anterior were evaluated by the ratio of left and right sides. Sciatic functional index (SFI) was also evaluated.

Results

The wet muscle weight was significantly better in the SVF group than in the control group. The results of distal latency, amplitude, and SFI were not significantly different between the two groups; however, these results tended to be better in the SVF group than in the control group.

Conclusion

SVF added to artificial nerve grafts has been reported to promote axonal regeneration through secretion of angiogenic, neurotrophic, and anti-apoptotic factors. This study indicates that SVF may also be effective for nerve autografts and improve the clinical result of nerve autograft.

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Acknowledgements

We thank Ms. Yoko Kasai for her skillful technical assistance in the immunohistochemical analysis of tissue specimens.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-8641, Japan

    Kaoru Tada, Mika Nakada, Masashi Matsuta, Atsuro Murai, Katsuhiro Hayashi & Hiroyuki Tsuchiya

Authors
  1. Kaoru Tada
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  2. Mika Nakada
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  3. Masashi Matsuta
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  4. Atsuro Murai
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  5. Katsuhiro Hayashi
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  6. Hiroyuki Tsuchiya
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Contributions

Conceptualization was done by KT; Methodology was done by MN, MM, KT; Investigation was done by M.N, M.M, A.M; Writing—Original Draft was done by K.T; Writing—Review & Editing were done by K.H, H.T; Supervision was done by K.T, H.T; Project Administration was done by K.T, H.T.

Corresponding author

Correspondence to Kaoru Tada.

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Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

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Availability of data and material

The datasets during and/or analyzed during the current study available from the corresponding author on reasonable request.

Ethics approval

This study was approved by the Advanced Science Research Center of our University (approval number: AP-194108). Surgery was performed under clean operation in compliance with the guidelines of the Ethics Committee of the International Association for the Study of Pain.

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Tada, K., Nakada, M., Matsuta, M. et al. Enhanced nerve autograft using stromal vascular fraction. Eur J Orthop Surg Traumatol 31, 183–188 (2021). https://doi.org/10.1007/s00590-020-02758-4

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  • DOI: https://doi.org/10.1007/s00590-020-02758-4

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