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Intraneural OX7-saporin for neuroma-in-continuity in a rat model

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European Journal of Orthopaedic Surgery & Traumatology Aims and scope Submit manuscript

Abstract

We employed 54 rats to devise a model of neuroma-in-continuity and explore the effect of the immunotoxin OX7-saporin on the neuroma. The left common peroneal, tibial or sciatic nerves were crushed by one 10-s application of a micro-artery forceps. At 3 and 6 weeks, the nerve was cut distal to the site of nerve crush, and retrograde fluorescent labeling was done. Pressure microinjection of 2 μl of natural saline or 2 μl of the immunotoxin conjugate OX7-saporin was done at the nerve stump 2 days later. Sacrifice was done after 3 weeks. In all control and saline-injection nerve specimens, gross observation and histology showed a neuroma-in-continuity. In 19 of the 24 OX7-saporin nerve specimens, gross observation showed a narrowed area at the site of nerve crush. Histology showed inhibition of neuroma-in-continuity formation. Fluorescent microscopy showed ablation of the labeled neurons in the dorsal root ganglia corresponding to the OX7-saporin subgroups.

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Acknowledgments

We thank Dr. Ismene A. Dontas, veterinary surgeon, and Mrs. Kalliopi Kalogera and Chrysoula Kapsis, chief nurses of the Laboratory for the Research of Musculoskeletal System “Th. Garofalidis,” for their significant contribution and technical assistance for this study. Rules for animal experimentation, handling and care, and ethical approval for this project were granted through the Animal Investigational Committee.

Conflict of interest

No funds were received in support of this study.

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Authors and Affiliations

  1. First Department of Orthopaedics, Athens University Medical School, 41 Ventouri Street, 15562, Holargos, Athens, Greece

    Andreas F. Mavrogenis, Anna Stamatoukou, Panayiotis J. Papagelopoulos & Panayotis N. Soucacos

  2. Department of Pathology, Athens University Medical School, Athens, Greece

    Kitty Pavlakis & Stamatis Theoharis

  3. Department of Neurosurgery, School of Medicine, John Hopkins University, Baltimore, ML, USA

    Zijie Ζhang

  4. First Department of Orthopaedics, Athens University Medical School, 1 Rimini Street, 12462, Haidari, Athens, Greece

    Aristides B. Zoubos

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  1. Andreas F. Mavrogenis
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  2. Kitty Pavlakis
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  4. Panayiotis J. Papagelopoulos
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  5. Stamatis Theoharis
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  6. Zijie Ζhang
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  7. Panayotis N. Soucacos
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  8. Aristides B. Zoubos
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Correspondence to Andreas F. Mavrogenis.

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The animals were provided under licence by the Biomedical Research Foundation of the Academy of Athens.

The study was conducted at the Laboratory for the Research of Musculoskeletal System “Th. Garofalidis,” KAT Hospital, Kifissia, and the Pathology Laboratory, Department of Pathology, Athens University Medical School, Athens, Greece.

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Mavrogenis, A.F., Pavlakis, K., Stamatoukou, A. et al. Intraneural OX7-saporin for neuroma-in-continuity in a rat model. Eur J Orthop Surg Traumatol 23, 263–272 (2013). https://doi.org/10.1007/s00590-012-0996-x

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  • DOI: https://doi.org/10.1007/s00590-012-0996-x

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