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Diagnostic and prognostic significance of miR-486-5p in patients who underwent minimally invasive surgery for lumbar spinal stenosis

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Abstract

Background

This study aimed to investigate the expression and clinical value of microRNA miR-486-5p in diagnosing lumbar spinal stenosis (LSS) patients and predicting the clinical outcomes after minimally invasive spinal surgery (MISS) in LSS patients, and the correlation of miR-486-5p with inflammatory responses in LSS patients.

Methods

This study included 52 LSS patients, 46 patients with lumbar intervertebral disk herniation (LDH) and 42 healthy controls. Reverse transcription quantitative PCR was used to detect miR-486-5p expression. The ability of miR-486-5p to discriminate between different groups was evaluated by receiver-operating characteristic analysis. The visual analogue scale (VAS), Oswestry Disability Index (ODI) and Japanese Orthopaedic Association (JOA) scores at 6 months postoperatively were used to reflect the clinical outcomes of LSS patients. Enzyme-linked immunosorbent assay was used to measure the levels of inflammatory factor [interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)]. The correlation of miR-486-5p with continuous variables in LSS patients was evaluated by the Pearson correlation coefficient.

Results

Expression of serum miR-486-5p was upregulated in LSS patients and had high diagnostic value to screen LSS patients. In addition, serum miR-486-5p could predict the 6-month clinical outcomes after MISS therapy in LSS patients. Moreover, serum miR-486-5p was found to be positively correlated with the levels of IL-1β and TNF-α in patients with LSS.

Conclusion

miR-486-5p, increased in LSS patients, can function as an indicator to diagnose LSS and a predictive indicator for the clinical outcomes after MISS therapy in LSS patients. In addition, miR-486-5p may regulate LSS progression by modulating inflammatory responses.

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References

  1. Lee BH, Moon SH, Suk KS, Kim HS, Yang JH, Lee HM (2020) Lumbar spinal stenosis: pathophysiology and treatment principle: a narrative review. Asian Spine J 14(5):682–693

    Article  PubMed  PubMed Central  Google Scholar 

  2. Jensen RK, Jensen TS, Koes B, Hartvigsen J (2020) Prevalence of lumbar spinal stenosis in general and clinical populations: a systematic review and meta-analysis. Eur Spine J 29(9):2143–2163

    Article  PubMed  Google Scholar 

  3. Ishimoto Y, Yoshimura N, Muraki S, Yamada H, Nagata K, Hashizume H et al (2013) Associations between radiographic lumbar spinal stenosis and clinical symptoms in the general population: the Wakayama Spine Study. Osteoarthritis Cartilage 21(6):783–788

    Article  CAS  PubMed  Google Scholar 

  4. Zhang J, Liu TF, Shan H, Wan ZY, Wang Z, Viswanath O et al (2021) Decompression using minimally invasive surgery for lumbar spinal stenosis associated with degenerative spondylolisthesis: a review. Pain Ther 10(2):941–959

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Khandge AV, Sharma SB, Kim JS (2021) The evolution of transforaminal endoscopic spine surgery. World Neurosurg 145:643–656

    Article  PubMed  Google Scholar 

  6. Chen J, Yu X, Qiu M, Feng F, Liu Z, Zhong G (2021) Circular RNA expression profile in patients with lumbar spinal stenosis associated with hypertrophied ligamentum flavum. Spine(Phila Pa 1976) 46(17):E916–E25

    Article  PubMed  Google Scholar 

  7. Yamada T, Horikawa M, Sato T, Kahyo T, Takanashi Y, Ushirozako H et al (2021) Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with abnormal accumulation of specific lipids. Sci Rep 11(1):23515

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Sairyo K, Biyani A, Goel VK, Leaman DW, Booth R Jr, Thomas J et al (2007) Lumbar ligamentum flavum hypertrophy is due to accumulation of inflammation-related scar tissue. Spine (Phila Pa 1976) 32(11):E340–E347

    Article  PubMed  Google Scholar 

  9. Sun C, Tian J, Liu X, Guan G (2017) MiR-21 promotes fibrosis and hypertrophy of ligamentum flavum in lumbar spinal canal stenosis by activating IL-6 expression. Biochem Biophys Res Commun 490(3):1106–1111

    Article  CAS  PubMed  Google Scholar 

  10. Xu YQ, Zhang ZH, Zheng YF, Feng SQ (2016) MicroRNA-221 regulates hypertrophy of ligamentum flavum in lumbar spinal stenosis by targeting TIMP-2. Spine (Phila Pa 1976) 41(4):275–82

    Article  PubMed  Google Scholar 

  11. Chang Q, Ji M, Li C, Geng R (2020) Downregulation of miR4865p alleviates LPSinduced inflammatory injury, oxidative stress and apoptosis in Chondrogenic cell ATDC5 by targeting NRF1. Mol Med Rep 22(3):2123–2131

    Article  CAS  PubMed  Google Scholar 

  12. Chai X, Si H, Song J, Chong Y, Wang J, Zhao G (2019) miR-486-5p inhibits inflammatory response, matrix degradation and apoptosis of nucleus pulposus cells through directly targeting FOXO1 in intervertebral disc degeneration. Cell Physiol Biochem 52(1):109–118

    Article  CAS  PubMed  Google Scholar 

  13. Zhang J, Xu Z, Kong L, Gao H, Zhang Y, Zheng Y et al (2020) miRNA-486-5p promotes COPD progression by targeting HAT1 to regulate the TLR4-triggered inflammatory response of alveolar macrophages. Int J Chron Obstruct Pulmon Dis 15:2991–3001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25(4):402–408

    Article  CAS  PubMed  Google Scholar 

  15. Zhang G, Zhang W, Hou Y, Chen Y, Song J, Ding L (2018) Detection of miR29a in plasma of patients with lumbar spinal stenosis and the clinical significance. Mol Med Rep 18(1):223–229

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Li P, Liu C, Qian L, Zheng Z, Li C, Lian Z et al (2021) miR-10396b-3p inhibits mechanical stress-induced ligamentum flavum hypertrophy by targeting IL-11. FASEB J 35(6):e21676

    Article  CAS  PubMed  Google Scholar 

  17. Wei W, Liu C, Yao R, Tan Q, Wang Q, Tian H (2021) miR4865p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9. Mol Med Rep 24(5).

  18. Liu H, Ni Z, Shi L, Ma L, Zhao J (2019) MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1. Mol Cell Probes 44:37–43

    Article  CAS  PubMed  Google Scholar 

  19. Xiao J, Shen B, Li J, Lv D, Zhao Y, Wang F et al (2014) Serum microRNA-499 and microRNA-208a as biomarkers of acute myocardial infarction. Int J Clin Exp Med 7(1):136–141

    PubMed  PubMed Central  Google Scholar 

  20. Zhang Y, Wang H, Xia Y (2021) The expression of miR-211-5p in atherosclerosis and its influence on diagnosis and prognosis. BMC Cardiovasc Disord 21(1):371

    Article  PubMed  PubMed Central  Google Scholar 

  21. Li G, Wang Q, Li Z, Shen Y (2020) Serum miR-21 and miR-210 as promising non-invasive biomarkers for the diagnosis and prognosis of colorectal cancer. Rev Esp Enferm Dig 112(11):832–837

    CAS  PubMed  Google Scholar 

  22. Zhu YY, Wu RN, Li X, Chen XB (2021) Value of serum miR-922 and miR-506 expression levels in the diagnosis and prognostic assessment of childhood acute lymphoblastic leukemia. Zhongguo Dang Dai Er Ke Za Zhi 23(10):1021–1026

    PubMed  Google Scholar 

  23. Li C, Zheng X, Li W, Bai F, Lyu J, Meng QH (2018) Serum miR-486-5p as a diagnostic marker in cervical cancer: with investigation of potential mechanisms. BMC Cancer 18(1):61

    Article  PubMed  PubMed Central  Google Scholar 

  24. Zheng X, Xu K, Zhu L, Mao M, Zhang F, Cui L (2020) MiR-486-5p act as a biomarker in endometrial carcinoma: promotes cell proliferation, migration, invasion by targeting MARK1. Onco Targets Ther 13:4843–4853

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Sun B, Guo S (2021) miR-486-5p serves as a diagnostic biomarker for sepsis and its predictive value for clinical outcomes. J Inflamm Res 14:3687–3695

    Article  PubMed  PubMed Central  Google Scholar 

  26. Ninawe A, Guru SA, Yadav P, Masroor M, Samadhiya A, Bhutani N et al (2021) miR-486-5p: a prognostic biomarker for chronic myeloid leukemia. ACS Omega 6(11):7711–7718

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ren C, Chen H, Han C, Fu D, Zhou L, Jin G et al (2016) miR-486-5p expression pattern in esophageal squamous cell carcinoma, gastric cancer and its prognostic value. Oncotarget 7(13):15840–15853

    Article  PubMed  PubMed Central  Google Scholar 

  28. Luo Q, Zhu J, Zhang Q, Xie J, Yi C, Li T (2020) MicroRNA-486-5p promotes acute lung injury via inducing inflammation and apoptosis by targeting OTUD7B. Inflammation 43(3):975–984

    Article  CAS  PubMed  Google Scholar 

  29. Lin FY, Han ST, Yu WM, Rao T, Ruan Y, Yuan R et al (2022) microRNA-486–5p is implicated in the cisplatin-induced apoptosis and acute inflammation response of renal tubular epithelial cells by targeting HAT1. J Biochem Mol Toxicol 36:e23039

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

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Funding

Yantai science and technology plan project: 2021YD044.

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Authors and Affiliations

  1. The Second Department of Spine Surgery, Yantaishan Hospital, Yantai, 264003, Shandong, China

    Heqing Zhang, Dong Liu & Xiaoguang Fan

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  1. Heqing Zhang
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  2. Dong Liu
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  3. Xiaoguang Fan
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Correspondence to Xiaoguang Fan.

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Conflict of interest

The authors declare that they have no conflict of interests.

Ethics approval and consent to participate

The experimental procedures were all in accordance with the guidelines of the Ethics Committee of Yantaishan Hospital and were approved by the Ethics Committee of Yantaishan Hospital. This study complies with the Declaration of Helsinki.

A signed written informed consent was obtained from each patient.

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The data used and analyzed can be obtained from the corresponding author under a reasonable request.

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Zhang, H., Liu, D. & Fan, X. Diagnostic and prognostic significance of miR-486-5p in patients who underwent minimally invasive surgery for lumbar spinal stenosis. Eur Spine J (2024). https://doi.org/10.1007/s00586-024-08203-y

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  • DOI: https://doi.org/10.1007/s00586-024-08203-y

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