Abstract
Purpose
Oxidative stress in the vertebral endplates of patients with low back pain and Modic changes (MCs) (types I, II, and III) endplate changes on magnetic resonance imaging. 8-iso-prostaglandin F2α (8-iso-PGF2α) has been proposed as new indicator of oxidative stress. Raftlin, as an inflammatory biomarker, has been previously reported in inflammatory diseases. Oxidative stress plays an important role in various human diseases. This study was aimed to assess Raftlin and 8-iso-PGF2α levels in patients with MCs.
Methods
Patients with MCI, II, and III (n = 45) and age- and sex matched controls subjects (n = 45) were enrolled in this study. 8-iso-PGF2α and Raftlin levels in the serum samples of both groups were measured with enzyme-linked immunosorbent assay.
Results
In our study results, raftlin levels changed in parallel with prostaglandin levels (p < 0.05). Raftlin levels changed in parallel with prostaglandin levels (p < 0.05). The levels of 8-iso-PGF2α and Raftlin levels showed increase in patients with MCs and the control group (p < 0.05). In addition, a significant positive correlation was found between MC-I, MC-II, MC-III and Raftlin (r = 0.756, 0.733, 0.701 p < 0.001, respectively). A significant positive correlation was found between ISO (Respectively; r = 0.782, 0.712, 0.716 p < 0.001). In our evaluation between Raftlin and Iso, a significant positive relationship was determined. (r = 0.731, p < 0.001).
Conclusion
Our findings indicated that oxidative stress in patients with MC-I may be aggravated and it may cause an inflammation formation of the lesion areas in these patients. Also, the increased 8-iso-PGF2α and Raftlin levels in patients with MC-II and MC-III may be an adaptive response to against oxidative stress.
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References
Ay S, Evcik D (2008) Depression and quality of life in patients with chronic low back pain. New Med J 225:228–231
Guzman J, Esmail R, Karjalainen K, Malmivaara A, Irvin E (2001) Multidisciplinary rehabilitation for chronic low back pain: systematic review. BMJ 322:1511–1516
Capkin E, Karkucak M, Cakırbay H, Topbas M, Karaca A, Köse MM, Gökmen F (2015) The prevalence and risk factors of low back pain in the eastern Black Sea region of Turkey. J Back Musculoskelet Rehabil 28(4):783–787
Kutsal YG (2000) Modern Medicine Seminars 11 Low Back Pain. Gunes Medical Bookstore, Ankara
Bianchi M, Peterson CK, Pfirrmann CWA, Hodler J, Bolton J (2015) Are the presence of MODIC changes on MRI scans related to improvement in low back pain patients treated with lumbar facet joint injections? BMC Musculoskelet Disord 16:234–258
Kuisma M, Karppinen J, Niinimäki J (2006) A three-year follow-up of lumbar spine endplate (modic) changes. Spine 31(15):1714–1718
Modic MT, Steinberg PM, Ross JS, Masaryk TJ, Carter JR (1988) Degenerative disk disease: assessment of changes in vertebral body marrow with MR imaging. Radiology 166(1):193–199
Braithwaite I, White J, Saifuddin A, Renton P, Taylor BA (1998) Vertebral end-plate (Modic) changes on lumbar spine MRI: correlation with pain reproduction at lumbar discography. Eur Spine J 7(5):363–368
de Roos A, Kressel H, Spritzer C, Dalinka M (1987) MR imaging of marrow changes adjacent to end plates in degenerative lumbar disk disease. AJR Am J Roentgenol 149(3):531–534
Schmid G, Witteler A, Willburger R, Kuhnen C, Jergas M, Koester O (2004) Lumbar disk herniation: correlation of histologic findings with marrow signal intensity changes in vertebral endplates at MR imaging. Radiology 231(2):352–358
Girard CJ, Schweitzer ME, Morrison WB, Parellada JA, Carrino JA (2004) Thoracic spine disc-related abnormalities: longitudinal MR imaging assessment. Skeletal Radiol 33(4):216–222
Kjaer P, Leboeuf-Yde C, Korsholm L, Sorensen JS, Bendix T (2005) Magnetic resonance imaging and low back pain in adults: a diagnostic imaging study of 40-Year-old men and women. Spine 30:1173–1180
Peterson CK, Humphreys BK, Pringle TC (2007) Prevalence of modic degenerative marrow changes in the cervical spine. J Manipulative Physiol Ther 30(1):5–10
Lee W, Yoo H, Ku S, Kim S, Bae J (2014) Raftlin: a new biomarker in human sepsis. Inflammation 37:706–711
Avnioglu S, Güngör M, Kurutas E, Ozturk U, Demirhan I, Bakaris S, Velioglu H, Cankaya S, Yulug B (2022) The effect of resveratrol on sphingosine-1 and oxidative/ nitrosative stress in an experimental heart ischemia reperfusion model. Revista Romana de Medicina de Laborator 30(1):9–18. https://doi.org/10.2478/rrlm-2021-0036
Morrow JD, Harris TM, Roberts LJ (1990) Noncyclooxygenase oxidative formation of a series of novel prostaglandins: analytical ramifications for measurement of eicosanoids. Anal Biochem 184:1–10
Pratico D, Rokach J, Lawson J, FitzGerald GA (2014) F2-isoprostanes as indices of lipid peroxidation in inflammatory diseases. Chem Phys Lipids 128:165–171
Kaviarasan S, Muniandy S, Qvist R, Ikram SI (2004) F2-Isoprostanes as novel biomarkers for Type 2 diabetes: a review. J Clin Biochem Nutr 45:1–8
Milne GL, Yin H, Morrow JD (2008) Human biochemistry of the isoprostane pathway. J Biol Chem 283:15533–15537
Low ESH, Krishnaswamy G, Thumboo J (2019) Comparing the 1997 update of the 1982 American College of Rheumatology (ACR-97) and the 2012 systemic lupus international collaborating clinics (SLICC-12) criteria for systemic lupus erythematosus (SLE) classification: which enables earlier classification of SLE in an urban Asian population? Lupus 28(1):11–18. https://doi.org/10.1177/0961203318811599
Stein CM, Tanner SB, Award JA, Awad RJ, Morrow D (1996) Evidence of free-radical-mediated injury (isoprostane overproduction) in Scleroderma. Arthritis Rheum 39:1146–1150
Basu S, Whiteman M, Mattey DL, Halliwell B (2001) Raised levels of F(2)-isoprostanes and prostaglandin F(2alpha) in different rheumatic diseases. Ann Rheum Dis 60(6):627–631. https://doi.org/10.1136/ard.60.6.627.PMID:11350853;PMCID:PMC1753663
Albert HB, Manniche C (2007) Modic changes following lumbar disc herniation. Eur Spine J 16(7):977–982
Mok FP, Samartzis D, Karppinen J, Fong DY, Luk KD, Cheung KM (2016) Modic changes of the lumbar spine: prevalence, risk factors, and association with disc degeneration and low back pain in a large-scale population-based cohort. Spine J 16:32–41
Kurutas EB (2016) The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J 15(1):71. https://doi.org/10.1186/s12937-016-0186-5
Grulovic B, Pucelj MR, Krnić M, Kokić V (2013) Impact of prostaglandin F2-alpha and tumor necrosis factor-alpha (TNF-alpha) on pain in patients undergoing thermal balloon endometrial ablation. Coll Antropol 37(4):1185–1190
Helmersson J, Mattsson P, Basu S (2002) Prostaglandin F2α metabolite an F2-isoprostane excretion rates in migraine. Clin Sci 102:39–43
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No funds were received in support of this work. No relevant financial activities outside the submitted work. Permission was obtained by the non-invasive clinical research ethics committee of Kahramanmaras Sutcu Imam University.
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Demirhan, İ., Oner, E., Yuksel, Z. et al. Raftlin and 8-iso-prostaglandin F2α levels and gene network analysis in patients with Modic changes. Eur Spine J 32, 2368–2376 (2023). https://doi.org/10.1007/s00586-023-07757-7
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DOI: https://doi.org/10.1007/s00586-023-07757-7