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European Spine Journal

, Volume 26, Issue 1, pp 259–266 | Cite as

The aging disc: using an ovine model to examine age-related differences in the biomechanical properties of the intralamellar matrix of single lamellae

  • Danielle M. Stewart
  • Lauren A. Monaco
  • Diane E. Gregory
Original Article

Abstract

Purpose

To determine the effect of age on the biomechanical properties of the intralamellar matrix of single annulus fibrosus (AF) lamellae.

Methods

One intervertebral disc (IVD) was excised from five young (<12 months), five middle-aged (2–4 years) and five older (5–7 years) ovine lumbar spines. From each IVD, a maximum of four single AF lamellae samples were harvested: two from the anterior region and two from the posterior region. Tissues were mounted in a tensile testing apparatus such that tension was applied perpendicular to the orientation of the collagen fibers to isolate the intralamellar matrix. Variables of interest from the stress–strain relationship were: end of toe-region strain and corresponding stress, initial failure stress and strain, and elastic stiffness.

Results

When compared to the middle-aged and old samples, the intralamellar matrix of young AF samples displayed significantly higher stress values at the end of the end of toe-region (p = 0.008) and at initial failure (p = 0.002). Further, the young samples were stiffer than both middle-aged and old samples (p = 0.04).

Conclusions

This study was the first to show that the intralamellar matrix of single AF lamellae is weaker and more compliant in middle-aged and old ovine IVDs compared to young IVDs. These findings are likely a result of the remarkable age-related changes that occur that ultimately weaken the IVD as a whole.

Keywords

Mechanical properties Intralamellar matrix Annulus fibrosus Material testing Aging 

Notes

Acknowledgments

The authors wish to thank The Natural Sciences and Engineering Research Council of Canada (NSERC) for financial support.

Compliance with ethical standards

Conflict of interest

The authors of this paper declare that there are no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Danielle M. Stewart
    • 1
  • Lauren A. Monaco
    • 2
  • Diane E. Gregory
    • 1
    • 3
  1. 1.Department of Kinesiology and Physical EducationWilfrid Laurier UniversityWaterlooCanada
  2. 2.Department of BiologyWilfrid Laurier UniversityWaterlooCanada
  3. 3.Department of Health SciencesWilfrid Laurier UniversityWaterlooCanada

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