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Progression of lumbar spinal stenosis is influenced by polymorphism of thrombospondin 2 gene in the Korean population

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Abstract

Purpose

The aim of this study is to determine the contribution of thrombospondin 2 (THBS2) polymorphisms to the development and progression of lumbar spinal stenosis (LSS) in the Korean population.

Methods

We studied 148 symptomatic patients with radiographically proven LSS and 157 volunteers with no history of back problems from our institution. Magnetic resonance images were obtained for all the patients and controls. Quantitative image evaluation for LSS was performed to evaluate the severity of LSS. All patients and controls were genotyped for THBS2 allele variations using a polymerase chain reaction-based technique.

Results

We found no causal single nucleotide polymorphism (SNPs) in THBS2 that were significantly associated with LSS. Two SNPs (rs6422747, rs6422748) were over-represented in controls [P = 0.042, odds ratio [OR] = 0.55 and P = 0.042, OR = 0.55, respectively]. Haplotype analysis showed that the ‘‘AGAGACG’’ haplotype (HAP4) and ‘‘AAGGACG’’ haplotype (HAP5) were over-represented in severe LSS patients (P = 0.0147, OR = 2.02 and P = 0.0137, OR = 2.48, respectively). In addition, the ‘‘AAAGGGG’’ haplotype (HAP1) was over-represented in controls (P = 0.0068, OR = 0.30).

Conclusions

Although no SNPs in THBS2 were associated with LSS, haplotypes (HAP4 and HAP5) were significantly associated with progression of LSS in the Korean population, whereas another haplotype (HAP1) may play a protective role against LSS development.

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Abbreviations

DSCSA:

Dural sac cross-sectional area

ECM:

Extracellular matrix

LRP:

Lipoprotein receptor-related protein

LSS:

Lumbar spinal stenosis

MMP:

Matrix metalloproteinase

MRI:

Magnetic resonance imaging

PCT:

Polymerase chain reaction

SNP:

Single nucleotide polymorphism

THBS:

Thrombospondin

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Acknowledgments

The manuscript submitted does not contain information about medical device(s)/drug(s). This study was supported by a Grant (2010-204) from the Asan Institute for Life Sciences, Seoul, Republic of Korea.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Spine Center, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Republic of Korea

    Seung-Jae Hyun

  2. Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea

    Borae G. Park

  3. Department of Neurosurgery, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-2dong, Songpa-Gu, Seoul, 138-736, Republic of Korea

    Seung-Chul Rhim, Sang-Ryong Jeon & Sung-Woo Roh

  4. Department of Neurosurgery, Nanoori Hospital, Incheon, Republic of Korea

    Jun-Won Jang

Authors
  1. Seung-Jae Hyun
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  2. Borae G. Park
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Corresponding author

Correspondence to Seung-Chul Rhim.

Additional information

S.-J. Hyun and B. G. Park equally contributed to the work as a co-first author.

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Hyun, SJ., Park, B.G., Rhim, SC. et al. Progression of lumbar spinal stenosis is influenced by polymorphism of thrombospondin 2 gene in the Korean population. Eur Spine J 23, 57–63 (2014). https://doi.org/10.1007/s00586-013-2866-6

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  • DOI: https://doi.org/10.1007/s00586-013-2866-6

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