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Pulsed radiofrequency effects on the lumbar ganglion of the rat dorsal root: a morphological light and transmission electron microscopy study at acute stage

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Abstract

Since the dorsal root ganglia represent the first structure of pain modulation, they are the target of the newest therapies of neuropathic pain. Between these, pulsed radiofrequency (PRF) has been described among the promising non-invasive methods. Although the results encourage the clinical use of this procedure, their mechanism of action is still unclear. Aim of our study was to analyze acute effects of PRF on the rat lumbar ganglion and on nervous fibres running inside it. Clinical works describe PRF treatment as a techinque without any visible neurological deficit. The few disposable histological works are contractictory: some describe no signs of cellular damage and some demonstrate visible intracellular modifications. A total of 20 male Wistar rats were deeply anesthesized. Ten were positioned in a stereotactic system, and exposed to PRF at 2 Hz for 30 s after exposition of paravertebral muscles and positioning of a stimulation needle on left L4 ganglion. The other ten were used as controls. After 1 h, the left dorsal root ganglions L3, L4, L5 of the 20 animals were explanted, fixed in 2.5% Karnowsky solution and prepared for light and transmission electron microscopy. At light microscopy no differences between treated and control animals were observed; at transmission electron microscopy, instead, it was possible to observe that T gangliar cells contained an abnormal abundant smooth reticulum with enlarged cisternae and numerous vacuoles; myelinated axons presented pathological features and their myelin coverage was not adherent. Instead, unmyelinated axons appeared normal in shape and dimension and the Schwann cells surrounding it had intact plasmamembrane. Our results, obtained at acute stage, reveal that the PRF procedure should destroy the myelin envelope of nervous fibres. Further future studies, at chronic stage, should give other information on the prognosis of the myelinic damage.

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Acknowledgments

The authors gratefully acknowledge the “Centro Grandi Attrezzature per la Ricerca Biomedica” of Insubria University for instruments availability.

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Authors and Affiliations

  1. Department of Human Morphology, University of Insubria, via Monte Generoso 71, 21100, Varese, Italy

    Marina Protasoni, Marcella Reguzzoni & Carlo Dell’Orbo

  2. Department of Surgery, Neurosurgical Unit, University of Insubria, viale Borri 57, Varese, Italy

    Simone Sangiorgi, Alessandro Dario & Giustino Tomei

  3. Pain Unit Treatment, Oglio-Po Hospital, via Staffolo, 51, Vicomoscano, Casalmaggiore (CR), Italy

    Claudio Reverberi

  4. Unit of Human Anatomy, Department of Biomedical Sciences and Biotechnologies, University of Brescia, Piazzale Spedali civili 1, Brescia, Italy

    Elisa Borsani & Luigi F. Rodella

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  1. Marina Protasoni
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  2. Marcella Reguzzoni
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  9. Carlo Dell’Orbo
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Corresponding author

Correspondence to Marina Protasoni.

Additional information

Morphological PRF effects on ganglions.

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Protasoni, M., Reguzzoni, M., Sangiorgi, S. et al. Pulsed radiofrequency effects on the lumbar ganglion of the rat dorsal root: a morphological light and transmission electron microscopy study at acute stage. Eur Spine J 18, 473–478 (2009). https://doi.org/10.1007/s00586-008-0870-z

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  • DOI: https://doi.org/10.1007/s00586-008-0870-z

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