Abstract
The hazardous elements contained in chemical fertilizers may not only potentially cause adverse health effects to the environment but also to professional health. The current study has been conducted to evaluate the probable effects of chemical fertilizers on occupational health. Forty male participants have been divided into two main groups, the exposed group (workers) includes 20 employees working in the fertilizer industry of Annaba (Algeria) for at least 6 years; the control group includes 20 participants with no history of exposure to any chemical fertilizer. Blood samples have been collected for biochemical, hematological, and hormonal analysis and to evaluate oxidative stress status; urine samples have been used to determine chemical components of urine. Data of biochemical analysis have shown a significant increase in serum cholesterol and triglyceride levels (TG) (P = 0.037; P = 0.014, respectively), a very significant increase (P = 0.001) in liver enzymes aspartate aminotransferase (AST), and alanine aminotransferase (ALT); a very significant increase in kidney function markers: creatinine (P = 0.001) and urea (P = 0.003). On the other hand, the result of hematological analysis has shown a significant decrease (P = 0.013) in hemoglobin (Hgb) concentration and a very significant decrease (P = 0.001) in erythrocyte count. No significant difference has been recorded in hormonal parameters between workers and the control group; concerning oxidative stress status, we have recorded a very significant decrease in antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)). Taken together, occupational exposure to chemical fertilizer can induce many adverse health effects, illustrated mainly by kidney and liver function impairments, and can also cause deleterious impacts on the biomarkers of oxidative stress status.
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References
Aebi H (1984) Catalase in Vitro Methods Enzymology 105:121–126
Ajmal M, Ali HI, Saeed R, Akhtar A, Tahir M et al (2018) Biofertilizer as an alternative for chemical fertilizers. Journal of Agriculture and Allied Sciences 7(1):1–7
Al-Afaleg NO, Al-Senaidy A, El-Ansary A (2011) Oxidative stress and antioxidant status in Saudi asthmatic patients. Clin Biochem 44(8–9):612–617
Aster JC (2004) Anaemias of diminished erythropoiesis. In: Kumar V, Abbas AK, Fausto N (eds) Robbins & Cotran Pathologic Basis of Disease. Saunders, Philadelphia, pp 638–649
Beier S, Classen HG, Loeffler K, Schumacher E, Thöni H (1995) Antihypertensive effect of oral nitrite uptake in the spontaneously hypertensive rat. Arzneimittelforschung 45(3):258–261
Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161(2):559–566
Bhardwaj H, Singh C, Nayyar S (2020) Assessment of adverse effects of lead, nickel and cadmium on biochemical parameters, antioxidants status and metallothionein expression in buffaloes slaughtered at local abattoir. Indian J Anim Res 56(2):145–152
Bouaziz H, Rafrafi M, Ben Salah G, Marrekchi R, Boudawara O (2015) Nitrate provoked kidney toxicity and DNA impairment in adult rats: alleviation by Hyparrhenia hirta. Pak Vet J 35:397–402
Boukerche S, Aouacheri W, Saka S (2007) Toxicological effects of nitrate: biological study in human and animals. Ann Biol Clin 65(4):385–391
Bradford MA (1976) Rapid and sensitive method for the quantities of microgram quantities of protein utilizing the principle of protein binding. Anal Biochem 72:248–254
Chien SH, Prochnow LI, Cantarella H (2009) Recent development of fertilizer production and use to improve nutrient efficiency and minimize environmental impacts. Adv Agron 102:267–322
Damian A, Ghita M, Zagrai G, Dumitrescu I, Vasilescu C et al (2020) Research regarding the effect of vitamin E on some hematological parameters in toxic stress conditions in rats. Bulletin UASVM Veterinary Medicine 77:21–24
Esterbauer H, Gebicki J, Puhl H, Jürgens G (1992) The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Radical Biol Med 13(4):341–390
Filiopoulos V, Vlassopoulos D (2009) Inflammatory syndrome in chronic kidney disease: pathogenesis and influence on outcomes. Inflammation & Allergy-Drug Targets (Formerly Current Drug Targets-Inflammation &Allergy) (Discontinued ) 8(5):369–382
Flohé L, Günzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114–121
Fouad SS, Mohi-Eldin MM, Haridy MA, Khalil KM (2017) Ameliorative effects of ascorbic acid (Vit. C) against sodium nitrite toxicity in albino rats: hematological, biochemical and histopathological Studies. AEJTS 9:01–06
Geetha B, Nair RH, Kesavachandran C, Chandy S, Shashidhar S (2001) Pulmonary function in workers of fertilizer and chemical industry. Indian J Physiol Pharmacol 45(2):215–221
Gorman NGM, Stjernberg E, Koehoorn M, Demers PA, Winters M et al (2013) Fertilizer use and self-reported respiratory and dermal symptoms among tree planters. J Occup Environ Hyg 10(1):36–45
Guastadisegni C, Mantovani A, Ricciardi C, Stazi AV, Maffi D et al (1989) Hematotoxic effect in the rat of a toluenedinitro derivate after short-term exposure. Ecotoxicol Environ Saf 17(1):21–29
Huang WH, Lin JL (2004) Acute renal failure following ingestion of manganese containing fertilizer. J Toxicol Clin Toxicol 42:305–307
Ihedioha JI and Idika IK (2007) Hematological abnormalities associated with contamination of drinking water with low sub-lethal concentrations of inorganic fertilizers. Proceedings of 32nd Annual Conference of Nigerian Society for Animal Production, (Eds Agiang EA, Agwunobi LN and Olawoyin OO) March 18–21, 2003, Calabar, Nigeria, pp. 27–30
Ihedioha JI, Okorie Kanu CO (2011) Clinicopathologic findings in goats exposed to drinking water experimentally contaminated with varied low percentages of NPK 15-15-15 fertilizer. Comp Clin Pathol 20:349–358
Ilieva I, Sainova I (2022) Free radicals and oxidative stress as the main mechanism of heavy metal toxicity in the male reproductive system. Acta MorphologiqueEt Anthropologica 29:1–2
Jayasumana Ch, Fonseka S, Fernando A, Jayalath K, Amarasinghe M et al (2015) Phosphate fertilizer is a main source of arsenic in areas affected with chronic kidney disease of unknown etiology in Sri Lanka. Springer plus 4(90):2–8
Joshi D, Kumar Md, Kumar SA, Sangeeta S (2014) Reversal of methylmercuryinduced oxidative stress, lipid peroxidation, and DNA damage by the treatment of N-acetyl cysteine: a protective approach. J Environ Pathol Toxicol Oncol 33(2):167–182
Kamalakkanan N, Prince PSM (2004) Antidiabetic and anti-oxidant activity of Aegle marmelos extract in streptozotocin-induced diabetic rats. Pharm Biol 42:125–130
Kamel NM, Abou El-Wafa HS, Al-Wehedy A (2017) Occupational injuries among workers in a nitrogenous fertilizers company, Egypt. Egypt J Occup Med 41(1):1–17
Khan MMA, Halim ZI, Iqbal M (2006) Attributes of occupational injury among workers in the chemical industry and safety issues. Int J Occup Saf Ergon 12(3):327–341
Klibet F, Boumendjel A, Khiari M, El Feki A, Abdenour C, Messarah M (2016) Oxidative stress-related liver dysfunction by sodium arsenite: alleviation by Pistacia lentiscus oil. Pharm Biol 54(2):354–363
Lazarov A, Yair M, Lael E, Baitelman L (2002) Airborne irritant contact dermatitis from phosphates in a fertilizer factory. Contact Dermatitis 46(1):53–54
Loukil B, Mallem L, Maamer H, Boulakoud MS (2014) The study of risk associated with handling of fertilizer in workplace on some lipid and hormone parameters. World Appl Sci J 32(6):1159–1162
Mallem L, Loukil B, Boulakoud MS (2015) L’effet des engrais sur la santé professionnelle au travail. Ecophysiologie Et Toxicologie 3(1):16–21
Manfo FPT, Mboe SA, Nantia EA, Ngoula F, Telefo PB et al (2020) Evaluation of the effects of agro pesticides use on liver and kidney function in farmers from Buea, Cameroon. J Toxicol 2020:1–10
Maurya GK, Malick C, Mondal R, Gambhir L (2016) Study of acute toxicity of inorganic fertilizer NPK (15:15:15) on catfish, Heteropneustes fossilis. Int J Appl Biol Pharm Technol 7:243–253
Menadi S, Boubidi FS, Messarah M, Boumendjel A (2023) Biochemical, hematological and histopathological alterations in liver and lung of rats due to sub-chronic inhalation exposure to NPK 15.15. 15 fertilizer. Utter Pradesh Journal of Zoology 44(15):1–12
Neghab M, Mirzaei A, Jalilian H, Jahangiri M, Zahedi J (2019) Effects of low-level occupational exposure to ammonia on hematological parameters and kidney function. Int J Occup Environ Med 10(2):80–88
Nganchamung T, Robson MG, Siriwong W (2017) Chemical fertilizer use and acute health effects among chili farmers in Ubon Ratchathani province. Thailand Journal of Health Research 31(6):427–437
Olooto WE (2017) Infertility in male; risk factors, causes and management. A Review J Microbial Biotechnol Res 2:641–645
Poreba R, Gac P, Poreba M, Andrzejak R (2011) Environmental and occupational exposure to lead as a potential risk factor for cardiovascular disease. Environ Toxicol Pharmacol 3(1):267–277
Rakers F, Rupprecht S, Dreiling M, Bergmeier C, Witte OW et al (2020) Transfer of maternal psychosocial stress to the fetus. Neurosci Biobehav Rev 117:185–197
Rouag M, Berrouague S, Djaber N, Khaldi T, Boumendjel M, Taibi F, Abdennour C, Boumendjel A, Messarah M (2020) Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach. Afr Health Sci 20(1):413–425
Sato A, Gonmori K, Yoshioka N (1999) A case of fatal intoxication with ammonium sulfate and a toxicological study using rabbits. Forensic Sci Int 101:141–149
Savci S (2012) An agricultural pollutant: chemical fertilizer. Int J Environ Sci Dev 3(1):77–80
Schnurer A, Norberg A (2008) Ammonia, a selective agent for methane production by syntrophic acetate oxidation at mesophilic temperature. Water Sci Technol 57:735–740
Sharma A, Chetani R (2017) A review on the effect of organic and chemical fertilizers on plants. IJRASET 5(2):677–680
Sutton P, Woodruff TJ, Perron J, Stotland N, Conry JA et al (2012) Toxic environmental chemicals, the role of reproductive health professionals in preventing harmful exposures. Am J Obstet Gynecol 207(3):164–173
Talas ZS, Gogebakan A, Orun I (2013) Effects of propolis on blood biochemical and hematological parameters in nitric oxide synthase inhibited rats by N-Nitro-L-argininemehyl ester. Pak J Pharm Sci 26:915–919
Testud F (2004) Inorganic Fertilizers. EMC-Toxicologie-Pathologie 1(1):21–28
Valko M, Jomova K, Rhodes CJ, Kuča K, Musílek K (2016) Redox-and non-redox-metal-induced formation of free radicals and their role in human disease. Arch Toxicol 90:1–37
Vasconcelos LH, Ferreira SR, Silva MDC, Ferreira PB, Souza IL et al. (2021) Uncovering the role of oxidative imbalance in the development and progression of bronchial asthma. Oxidative Medicine and Cellular Longevity 2021.
Willson TM, Kliewer SA (2002) PXR, CAR and drug metabolism. Nat Rev Drug Discov 1:259–266
Zurovsky Y, Haber C (1995) Antioxidants attenuate endotoxin-generation induced acute renal failure in rats. Scand J Urol Nephrol 29:147–154
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Menadi, S., Boubidi, F.S., Bouchelaghem, R. et al. Occupational exposure to the dust of chemical fertilizers (NPK 15.15.15): effect on biochemical parameters and oxidative stress status among workers in Annaba. Comp Clin Pathol (2024). https://doi.org/10.1007/s00580-024-03563-9
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DOI: https://doi.org/10.1007/s00580-024-03563-9