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Cajanus cajan induces mitochondrial-mediated apoptosis via caspase activation and cytochrome c release

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Abstract

Background

Attention has recently focused on mitochondrial-mediated apoptosis as a signaling pathway that is disrupted in many diseases involving excessive cell proliferation. Medicinal plants that induce apoptosis may be useful in chemoprevention. Cajanus cajan (C. cajan) is cultivated in East and West Africa. It is used in folk medicine for the treatment of liver injury, anemia, dysentery, measles, jaundice, and tumor.

Purpose

This study aims to investigate the in vitro antioxidant and mitochondrial-mediated apoptotic effects of C. cajan.

Methods

The mitochondrial permeability transition (mPT), mitochondrial ATPase activity, (2, 2-diphenylpicrylhydrazyl) DPPH radical-scavenging activity, total antioxidant capacity, and total phenol and apoptotic biomarkers were assessed while the phytochemicals present in the extract were determined by gas chromatography-mass spectrometry (GC–MS). Methanol extract of Cajanus cajan (MECC) triggered mitochondrial-mediated apoptosis by induction of pore opening, enhancement of mitochondrial ATPase activities, and increased immunohistochemical expression of caspase 3, caspase 9, and cytochrome c.

Results

MECC triggered mitochondrial-mediated apoptosis by induction of pore opening and increased expression of caspase 3, caspase 9, and cytochrome c. Enhancement of ATPase activities inhibition of lipid peroxidation and DPPH scavenging activity of DPPH were observed.

Conclusions

These findings suggest that C. cajan-induced mitochondrial-mediated apoptosis in normal rat liver through the induction of mPT and eventual release of cytochrome c which is a prelude to the progression of apoptosis. Hence, certain bioactive components of C. cajan may be useful in chemoprevention and management of conditions associated with insufficient apoptosis.

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Authors and Affiliations

  1. Department of Biochemistry, University of Ibadan, Ibadan, Oyo State, Nigeria

    Olajumoke Nwaechefu, Idris Lateef & Olufunso Olorunsogo

  2. Department of Biochemistry, Lead City University, Ibadan, Oyo State, Nigeria

    Olajumoke Nwaechefu & Basirat Adeoye

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  1. Olajumoke Nwaechefu
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  2. Basirat Adeoye
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  3. Idris Lateef
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  4. Olufunso Olorunsogo
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Correspondence to Olajumoke Nwaechefu.

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Nwaechefu, O., Adeoye, B., Lateef, I. et al. Cajanus cajan induces mitochondrial-mediated apoptosis via caspase activation and cytochrome c release. Comp Clin Pathol 33, 207–222 (2024). https://doi.org/10.1007/s00580-023-03541-7

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