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Evaluation of combined antioxidant potential of p-coumaric acid and/or lisinopril — in vitro and in vivo

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Abstract

p-coumaric acid (p-CA) is a phenolic acid abundantly present in several foods, and lisinopril is a widely employed angiotensin-converting enzyme inhibitor for the treatment of hypertension and endothelial dysfunction. This study was convened to determine the combined antioxidative effect of p-CA and/ lisinopril — in vitro and in vivo. Stock solutions of p-CA (50 mM) and lisinopril (50 mM) were separately prepared. Subsequently, preparation of different combinations of samples (50% p-CA: 50% lisinopril; 75% p-CA: 25% lisinopril; 25% p-CA: 75% lisinopril, 100% p-coumaric acid and 100% lisinopril) was carried out for the determination of in vitro antioxidant properties as typified by DPPH, ABTS, and hydroxyl radical scavenging abilities, Fe2+ chelating ability, reducing property, and inhibition of Fe2+-induced lipid peroxidation. Furthermore, p-coumaric acid (50 and 100 mg/kg) and/or lisinopril (10 mg/kg) were administered orally (p.o.) to rats daily for 14 days. Then, the liver was excised, blood was collected, and the plasma was subsequently prepared. The antioxidant status was assessed by determining catalase, superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase activities as well as the levels of reduced glutathione, total thiol, and lipid peroxidation in rat liver. Combination of p-coumaric acid and lisinopril influenced the antioxidant capacity of lisinopril in vitro, improved antioxidant defense status when compared to control in vivo, and significantly decreased lipid peroxidation in vitro and in vivo. Results from this study indicate that the combination approach of p-CA and lisinopril could represent crucial alternative therapy in the management of free radical mediated damage.

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Authors and Affiliations

  1. Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, P.M.B. 704, Akure 340001, Nigeria

    Stephen A. Adefegha, Oluwatumininu O. Adesua & Ganiyu Oboh

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  1. Stephen A. Adefegha
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  2. Oluwatumininu O. Adesua
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  3. Ganiyu Oboh
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Contributions

SAA envisioned the study, contributed to its draught and execution, and assisted to prepare the manuscript; OOA participated in the experimental and data acquisition, study framework, organization, and data analysis, and assisted to prepare the manuscript; GO contributed to the study framework, organization, and interpretation and analysis of data.

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Correspondence to Oluwatumininu O. Adesua.

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The authors declare that they have no conflict of interest.

Ethical approval

All animals received humane care in accordance to the guide for the care and the use of laboratory animals prepared by the National Academy of Science and published by the National Institute of Health (USA) (National Research Council (USA) Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 2011). The Centre for Research and Development approved the guidelines for the protection of animals’ welfare during experiment and assigned the ethical number, FUTA/ETH/22/21.

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We, Stephen A. Adefegha, Oluwatumininu O. Adesua, and Ganiyu Oboh, give our consent for the publication of identifiable details within the manuscript to be published in the above journal and article.

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Adefegha, S.A., Adesua, O.O. & Oboh, G. Evaluation of combined antioxidant potential of p-coumaric acid and/or lisinopril — in vitro and in vivo. Comp Clin Pathol 32, 1035–1043 (2023). https://doi.org/10.1007/s00580-023-03514-w

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