Abstract
Aging process is associated with higher inflammatory and cardiovascular risk factors. Remote ischemic preconditioning and high-intensity interval training have been known as useful strategies for health problem prevention and management. The aim of this study was to investigate the influence of remote ischemic preconditioning and high-intensity interval training on cardiac troponin I, creatine kinase-MB, IL10, and C-reactive protein in middle-aged male Wistar rats. Twenty-four male Wistar rats (12-month-old) were randomly assigned to four groups: high-intensity interval training (HIIT), remote ischemic preconditioning + high-intensity interval training (RIPC + HIIT), sham-high-intensity interval training (Sham HIIT), and sham-remote ischemic preconditioning + high-intensity interval training (Sham RIPC + HIIT). The rats in the experimental groups were administered to the selected protocols of 3 days a week for 21 days. Then, animals were sacrificed 48 h following the last session, and blood samples were collected and analyzed with ELISA method for the cardiac troponin I, creatine kinase-MB, IL10, and C-reactive protein. Both of the selected protocols led to a significant decrease in CKMB and IL10; however, the cTnI elevated meaningfully in RIPC + HIIT compared to the sham groups, and CRP was not significantly different between the groups (P < 0.05). This study demonstrated that 3 weeks of HIIT did not significantly alter cTnI and CRP and caused a reduction in CK-MB as a myocardial injury biomarkers in middle-aged male Wistar rats. On the other hand, applying RIPC caused a significant elevation in cTnI. Therefore, these physiological adaptations can improve ischemic tolerance and inhibit myocardial infarction.
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Fereshteh, S., Mohammad, K., Saeed, K.B. et al. RIPC and HIIT affect inflammatory and cardiac injury biomarkers in middle-aged rats. Comp Clin Pathol 32, 547–551 (2023). https://doi.org/10.1007/s00580-023-03463-4
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DOI: https://doi.org/10.1007/s00580-023-03463-4