Abstract
Hashimoto’s thyroiditis (HT) is an autoimmune disease of the thyroid gland. Free radicals have been reported to be responsible for the complications observed in the pathogenesis of thyroid diseases and the later stages of the disease. Myeloperoxidase (MPO), which is released from neutrophils during inflammation and oxidative stress and causes cell destruction, is a marker that plays a role in the development of cardiovascular disease associated with oxidative damage in the arterial wall. Paraoxonase-1(PON-1), which is one of the molecules that play a role in oxidant balance, is an enzyme that inhibits lipoprotein oxidation by hydrolyzing lipid peroxides in oxidized low-density lipoprotein(LDL) structure. We investigated the role of HDL-associated MPO and PON-1 in patients with HT in terms of coronary artery disease. Our study group consisted of 54 patients with Hashimoto’s diagnosis and 50 healthy individuals in the control group.MPO and PON-1 levels were determined spectrophotometrically. When the study groups were evaluated, MPO levels were significantly higher in patients with Hashimoto thyroiditis than in the control group (p < 0.05, p = 0.001). A negative correlation was obtained between MPO and PON-1 (r = − 0.685). The decrease in PON-1 activity and increase in MPO activity due to the hypothyroid effect increases lipid peroxide formation and accelerates oxidized LDL formation, which leads to a decrease in antioxidant capacity and the development of atherosclerosis. Since oxidative stress in thyroid diseases is also responsible for the complications observed in the later stages of the disease, we think that important data were obtained with this study in terms of both diagnosis and treatment.
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References
Aldridge WN (1953) Serum esterases. I. Two types of esterase (A and B) hydrolysing p-nitrophenyl acetate, propionate and butyrate, and a method for their determination. Biochem J 53(1):110–7
Baser H, Can U, Baser S, Yerlikaya FH, Aslan U, Hidayetoglu BT (2015) Assesment of oxidative status and its association with thyroid autoantibodies in patients with euthyroid autoimmune thyroiditis. Endocrine 48(3):916–923
Baskol G, Atmaca H, Tanriverdi F, Baskol M, Kocer D, Bayram F (2007) Oxidative stress and enzymatic antioxidant status in patients with hypothyroidism before and after treatment. Exp Clin Endocrinol Diabetes 115(8):522–526
Brown MS, Goldstein JL (1992) Koch’s postulates for cholesterol. Cell 71(2):187
Cebeci E, Oner FA, Usta M, Yurdakul S, Erguney M (2011) Evaluation of oxidative stress, the activities of paraoxonase and arylesterase in patients with subclinic hypothyroidism. Acta Biomed 82(3):214–222
Cheng D, Talib J, Stanley CP, Rashid I, Michaëlsson E, Lindstedt EL et al (2019) Inhibition of MPO (myeloperoxidase) attenuates endothelial dysfunction in mouse models of vascular ınflammation and atherosclerosis. Arterioscler Thromb Vasc Biol 39(7):1448–1457
Fenton HJJ (1984) Oxidation of tartaric acid in presence of iron. J Chem Soc 65:899–910
Goldstein JL, Ho YK, Basu SK, Brown MS (1979) Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc Natl Acad Sci USA 76(1):333–337
Gomaraschi M, Calabresi L, Franceschini G (2016) Protective effects of HDL against ıschemia/reperfusion ınjury. Front Pharmacol 25(7):2
Kato Y (2016) Neutrophil myeloperoxidase and its substrates: formation of specific markers and reactive compounds during inflammation. J Clin Biochem Nutr 58(2):99–104
Khan AA, Alsahli MA, Rahmani AH (2018) Myeloperoxidase as an active disease biomarker: recent biochemical and pathological perspectives. Med Sci (basel) 6(2):33
Klebanoff SJ, Clark FA (1978) The neutrophil: function and clinical disorders, 1. Elsevier/North Holland Biomedical Press, Amsterdam, The Netherlands, p 810
Klebanoff SJ, Rosen H (1978) The role of myeloperoxidase in the microbicidal activity of polymorphonuclear leukocytes. Ciba Found Symp 6–8;(65):263–84
Koç HB, Kaçar Y (2012) Paraoksonaz1 (Pon1) Enzimi ve Polimorfizmleri. Arşiv Kaynak Tarama Dergisi 21(1):27–41
Kosmas CE, Martinez I, Sourlas A, Bouza KV, Campos FN, Torres V et al (2018) High-density lipoprotein (HDL) functionality and its relevance to atherosclerotic cardiovascular disease. Drugs Context 28(7):212525
Mackness B, Mackness M (2019) Unexpectedly higher diazoxon hydrolysis by serum paraoxonase-1 in coronary heart disease. Clin Biochem 65:21–23
Mehanathan PB, Erusan RR, Shantaraman K, Kannan SM (2019) Antithyroid peroxidase antibodies in multinodular Hashimoto’s thyroiditis ındicate a variant etiology. J Thyroid Res 21(2019):4892329
Podrez EA (2010) Anti-oxidant properties of high-density lipoprotein and atherosclerosis. Clin Exp Pharmacol Physiol 37(7):719–725
Poncin S, Van Eeckoudt S, Humblet K, Colin IM, Gérard AC (2010) Oxidative stress: a required condition for thyroid cell proliferation. Am J Pathol 176(3):1355–1363
Ross R (1999) Atherosclerosis–an inflammatory disease. N Engl J Med 340(2):115–126
Rossi ED, Adeniran AJ, Faquin WC (2019) Pitfalls in thyroid cytopathology. Surg Pathol Clin 12(4):865–881
Ruggeri RM, Vicchio TM, Cristani M, Certo R, Caccamo D, Alibrandi A et al (2016) Oxidative stress and advanced glycation end products in Hashimoto’s thyroiditis. Thyroid 26(4):504–511
Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physiol 82(2):291–295
Tashian R, Shaw M (1962) Inheritance of an erythrocyte acetylesterase variant in man. Am J Hum Genet 14(3):295–300
Thomas T, Sreedharan S, Khadilkar UN, Deviprasad D, Kamath MP, Bhojwani KM et al (2014) Clinical, biochemical & cytomorphologic study on Hashimoto’s thyroiditis. Indian J Med Res 140(6):729–735
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160(1):1–40
Variji A, Shokri Y, Fallahpour S, Zargari M, Bagheri B, Abediankenari S, Alizadeh A, Mahrooz A (2019) The combined utility of myeloperoxidase (MPO) and paraoxonase 1 (PON1) as two important HDL-associated enzymes in coronary artery disease: which has a stronger predictive role? Atherosclerosis 280:7–13
Vivekanandan-Giri A, Byun J, Pennathur S (2011) Quantitative analysis of amino acid oxidation markers by tandem mass spectrometry. Methods Enzymol 491:73–89
Wang X, Hai C (2016) Novel insights into redox system and the mechanism of redox regulation. Mol Biol Rep 43(7):607–628
Yunoki K, Naruko T, Inaba M, Inoue T, Nakagawa M, Sugioka K et al (2013) Gender-specific correlation between plasma myeloperoxidase levels and serum high-density lipoprotein-associated paraoxonase-1 levels in patients with stable and unstable coronary artery disease. Atherosclerosis 231(2):308–314
Zhang R, Brennan ML, Fu X, Aviles RJ, Pearce GL, Penn MS, Topol EJ, Sprecher DL, Hazen SL (2001) Association between myeloperoxidase levels and risk of coronary artery disease. JAMA 286(17):2136–2142
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Uncu, G., Avcı, E., Avci, G.A. et al. The role of HDL-related myeloperoxidase (MPO) and paraoxonase 1 (PON1) in terms of coronary artery disease in Hashimoto’s thyroiditis. Comp Clin Pathol 32, 273–279 (2023). https://doi.org/10.1007/s00580-023-03439-4
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DOI: https://doi.org/10.1007/s00580-023-03439-4