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Diagnostic investigations of lumpy skin disease in crossbred-cattle infected with Theileria annulata infection

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Abstract

The current work was carried out to reveal-up the probable cause that increases the seriousness of lumpy skin disease (LSD) in crossbred dairy cattle in villages of Assiut and Sohag Governorates, Upper Egypt. The serious form of LSD infected twenty-eight crossbred dairy cows. Diagnosis is based on the clinical background and histopathology. All cows were adult and previously vaccinated against LSD using the sheep pox “Romanian-strain” vaccine. Clinically, enormous numbers of hideous necrotic dermal lesions, similar to starred-shaped craters, were the prominent signs of the diseased cases in association with systemic reactions anorexia pyrexia, tachycardia, tachypnea, and edematous lymphadenitis in superficial nodes. Detailed clinical findings are described. Histopathologically, fibrinoid vasculitis, with severe perivascular mononuclear inflammatory cellular infiltration and ambulance of eosinophilic intracytoplasmic inclusions in the dermis were conspicuously histopathological findings. Blood films and lymph smears of the diseased cases revealed both erythrocytic and the lymphocytic forms of Theileria infection. Theileria annulata was identified by polymerase chain reaction (PCR). Theileria annulata infection and LSD interactions were discussed. This tick-borne-protozoan is an endemic infection in our villages and plays a pivotal role in the frustration of the humoral and cell-mediated immune statuses of the infected cattle inducing reduction of the efficiency of the applied vaccine, and increasing the seriousness of the endemic lumpy skin disease. A national strategy for reducing or minimizing the infection rate with Theileria infection should warranted in paralleling with vaccination programs to avoid the insufficiency of immune responses. The current work concludes that bovine theileriosis is an endemic disease in our villages and the existence of Theileria annulata infection minimizes the efficiency of the vaccination programs and increases the seriousness of LSD in crossbred cattle. The existence of sporadic cases of cattle infected by LSD precipitates the disease as a circulating disease in our villages in Egypt.

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The data used in this manuscript is publicly available.

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References

  • Abdallah FM, El Damaty HM, Kotb GF (2018) Sporadic cases of lumpy skin disease among cattle in sharkia province, Egypt: genetic characterization of lumpy skin disease virus isolates and pathological findings. Vet World 11(8):1150–1158

    Article  CAS  Google Scholar 

  • Abera Z, Degefu H, Gari G, Kidane M (2015) Sero-prevalence of lumpy skin disease in selected districts of West Wollega zone, Ethiopia. BMC Vet Res 11:135–144

    Article  Google Scholar 

  • Ali AA, Esmat M, Attia H, Selim A, Abdel-Hamid YM (1990) Clinical and pathological studies on lumpy skin disease in Egypt. Vet Rec 127(22):549–550

    CAS  Google Scholar 

  • Allam AM, Elbayoumy MK, Abdel-Rahman EH, Hegazi AG, Farag TK (2020) Molecular characterization of the 2018 outbreak of lumpy skin disease in cattle in Upper Egypt. Vet World 13(7):1262–1268

    Article  CAS  Google Scholar 

  • Amin AA, ElMashad A, El-Nahas E (2015) Pathological and virological studies on an outbreak of lumpy skin disease among cattle in Kalubia Governorate- Egypt. J Adv Vet Res 5:165–175

    Google Scholar 

  • Babiuk S, Bowden T, Parkyn G, Dalman B, Manning L, Neufeld J, Embury-Hyatt C, Copps J, Boyle D (2008) Quantification of lumpy skin disease virus following experimental infection in cattle. Transbound Emerg Dis 55:299–307

    Article  CAS  Google Scholar 

  • Buller RM, Arif BM, Black DN, Dumbell KR, Esposito JJ, Lefkowitz EJ, McFadden G, Moss B, Mercer AA, Moyer RW, Skinner MA (2005) Family poxviridae. Virus taxonomy: classification and nomenclature of viruses. Eighth Report of the International Committee on Taxonomy of Viruses, pp 117–133

  • Davies FG (1991) Lumpy skin disease of cattle: a growing problem in Africa and the Near East. World Anim Rev 68(3):37–42

    Google Scholar 

  • Dobbelaere D, Heussler V (1999) Transformation of Leukocytes by Theileria parva and T. annulata. Annu Rev Microbiol 53(1):1–42

  • Dobbelaere D, Rottenberg S (2003) Theileria induced leukocyte transformation. Curr Opin Microbiol 6(4):377–382

    Article  CAS  Google Scholar 

  • D’Oliveira CM, Weide M, Miguel A, Habel A, Jacqiet F, Jongejan F, Weide M (1995) Detection of Theileria annulata in blood samples of carrier cattle by PCR. J Clinical Microbiol 33(10):2665–2669. https://doi.org/10.1128/jcm.33.10.2665-2669.1995

    Article  CAS  Google Scholar 

  • Elhaig MM, Selim A, Mahmoud M (2017) Lumpy skin disease in cattle: frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on Egyptian buffaloes. Onderstepoort J Vet Res 84(1):1–6

    Article  CAS  Google Scholar 

  • Ferrolho J, Domingos A, Campino L (2016) Cattle specific immune mechanisms used against the Protozoan Theileria annulata International Trends of. Immunity 4(2):45–49

    CAS  Google Scholar 

  • Gharbi M, Darghouth MA, Elati K, AL‐Hosary, AA, Ayadi O, Salih DA, El Hussein AM, Mhadhbi M, Khamassi Khbou, M, Hassan SM, Obara I, Ahmed LS, Ahmed J (2020) Current status of tropical theileriosis in Northern Africa: a review of recent epidemiological investigations and implications for control. Transbound Emerg Dis 67 Suppl 1:8–25

  • Ilhan T, Williamson S, Kirvar E, Shiels B, Brown CGD (1998) Theileria annulata: carrier state and immunity. Ann N Y Acad Sci 849:109–125. https://doi.org/10.1111/j.1749-6632.1998.tb11040.x

    Article  CAS  Google Scholar 

  • Irvin AD, Morrison WI (1987) Immunopathology, immunology and immunoprophylaxis of Theileria infections. In: Soulsby EJL (ed) Immune responses in parasitic infections: immunology, immunopathology, and immunoprophylaxis. Vol. III: Protozoa. Boca Raton, FL: CRC Press, pp 223–274

  • Jensen K, Makins GD, Kaliszewska A, Hulme MJ, Paxton E, Glass EJ (2009) The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors. Int J Parasitol 39:1099–1108

  • Maximous MRA (2019) Epidemiological and molecular study on theileria infection in ruminant in Al-Ghaniem, Assiut Governorate, Egypt. M.V.Sc., Infectious Diseases, Dept. Animal Med., Faculty of Vet. Med., Assiut Univ., Egypt

  • Preston PM, Darghouth M, Boulter NR, Hall RF, Tall R, Kirvar E, Brown DC (2002) A dual role for immunosuppressor mechanisms in infection with Theileria annulata: well-regulated suppressor macrophages help in recovery from infection; profound immunosuppression promotes non-healing disease. Parasitol Res 88:522–534

  • Rouby SR, Safwat NM, Hussein KH, Abdel- Ra’ouf AM, Madkour BS, Abdel-Moneim AS, Hosein HI (2021) Lumpy skin disease outbreaks in Egypt during 2017–2018 among sheeppox vaccinated cattle: epidemiological, pathological, and molecular findings. PLOS ONE. https://doi.org/10.1371/journal.pone.0258755

  • Rossiter PB, Al-Hammadi N (2008) Living with transboundary animal diseases (TADs). Trop Anim Health Prod 41:999–1004

    Article  Google Scholar 

  • Salib FA, Osman AH (2011) Incidence of lumpy skin disease among Egyptian cattle in Giza Governorate Egypt. Vet World 4(4):162–167

    Google Scholar 

  • Suvarna SK, Layton C, Bancroft JD (2013) Bancroft’s theory and practice of histological techniques, 7th edn. Churchill Livingstone, Elsevier, England

    Google Scholar 

  • Selim A, Manaa E, Khater H (2021) Seroprevalence and risk factors for lumpy skin disease in cattle in Northern Egypt Tropical Animal Health and Production. 53:350– 358

  • Tuppurainen ESM, Oura CAL (2012) Review: lumpy skin disease: an emerging threat to Europe, the Middle East and Asia. Transbound Emerg Dis 6:243–255

    Google Scholar 

  • Uilenberg G (1981) Theilerial species of domestic livestock. In: Irvin AD, Cunningham MP, Young AS (eds) Advances in the Control of Theileriosis. Martinus Nijhoff Publishers, The Hague, pp 4–37

    Chapter  Google Scholar 

  • Zaitoun AMA, Abdel-Rady A, Al-hosary AAT, Maximous MRA (2019) Clinical and pathological study of theileriosis in cattle and buffaloes in Al-Ghaneim region, Assiut governorate, Upper Egypt Assiut. Vet Med J 65(163):19–30

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Acknowledgements

The authors thank the veterinarians and farm owners for their support and help in providing data and sample collection throughout the study.

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Authors and Affiliations

  1. Infectious Diseases, Dept. of Animal Med., Faculty of Vet. Med., Assiut University, PO Box 71526, Assiut, Egypt

    Ahmed M. A. Zaitoun & Ahmed Abdel-Rady

  2. Faculty of Vet. Med., Dept. of Pathology & Clinical Pathology, Sohag University, Sohag, Egypt

    Fatma Abo Zakaib Ali

  3. Field Veterinarian, Assiut’s Veterinary Directorate, Assiut, Egypt

    Micheal R. Maximous

  4. Infectious Disease, Faculty of Vet. Med., Dept. Animal Med., South Valley University, Qena, Egypt

    Fatma A. Khalifa

Authors
  1. Ahmed M. A. Zaitoun
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  2. Fatma Abo Zakaib Ali
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  3. Micheal R. Maximous
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  4. Fatma A. Khalifa
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  5. Ahmed Abdel-Rady
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Contributions

All authors contributed to the study conception, design, material preparation, data collection, and analysis. All authors read and approved the final manuscript.

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Correspondence to Ahmed Abdel-Rady.

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This study was not supported by any funding.

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The authors declare that they have no conflict of interest.

Ethical approval

The ethical approval letter, (No.19 in 27/10/2022), according to ethical research committee members at Department of animal medicine, Faculty of Veterinary Medicine, South Valley University, approved the scientific research project (No. 91/27.10.2022) dated 27/10/2022.

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Zaitoun, A.M.A., Ali, F.A.Z., Maximous, M.R. et al. Diagnostic investigations of lumpy skin disease in crossbred-cattle infected with Theileria annulata infection. Comp Clin Pathol 32, 83–90 (2023). https://doi.org/10.1007/s00580-022-03415-4

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