Abstract
Chronic alcohol consumption has a negative impact on the submandibular and parotid salivary glands. The aim of this study was to study changes in morphological structures and to determine the relationship with the levels of neurotransmitters in the bioamine-containing structures of submandibular glands (SMGs) and parotid glands (PGs) in rats with prolonged alcohol intoxication. In this study, sexually mature male albino Wistar rats consumed 20% ethanol (6.9 g/kg/day) for 180 consecutive days. PGs and SMGs were collected for morphometric and luminescence-histochemical analyses (nonparametric Mann–Whitney U-test, p < 0.05). PGs exposed to ethanol for 180 days showed a change in the shape of the acini and secretory cells that formed them, uneven expansion of the interlobular excretory ducts, and moderate fatty infiltration of the stroma. SMGs exposed to ethanol for 180 days showed changes in acinar cells, intercalated and striated ducts, fatty infiltration, mild atrophy, and stromal edema. The number of mast cells in PGs and SMGs and their degranulation index doubled after exposure to ethanol, and the cells were highly active. With prolonged 180-day alcohol intoxication, there was a gradual increase in the histamine level in the acini of the salivary glands, a decrease in the serotonin level and catecholamines in all structures of the salivary glands, and a gradual decrease in the activity of monoamine oxidase to the level of control. Therefore, prolonged 180-day ethanol intoxication can cause negative morphological changes and functional differences in the activity of monoamine oxidase and biogenic amines in the SMGs and PGs of rats.
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The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
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All authors substantially contributed to the conception and the design of the work and agreed to be accountable for all aspects of the work. Olga Alexandrovna Sorkina, Marta Igorevna Sergushkina, and Olga Nurzadinovna Solomina carried out the acquisition of data for the work. Olga Alexandrovna Sorkina, Lyubov Mikhailovna Yakovleva, Tatiana Vitalievna Polezhaeva, and Oksana Olegovna Zaitseva analyzed and interpreted the data for the work. Tatiana Vitalievna Polezhaeva, Oksana Olegovna Zaitseva, and Andrey Nikolaevich Khudyakov drafted the article, and all authors revised it for important intellectual content, and read and approved the final article.
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All procedures were carried out in accordance with the Order of the Ministry of Health of the USSR dated 12.08.1977 No. 755 “On measures to further improve organizational forms of work using experimental animals” and with the Federal Law of the Russian Federation “On the protection of animals from cruelty” dated 01.12.1999 No. 4679-II GD. To conduct this study, permission was obtained from the University Ethics Committee (protocol no. 3 of November 16, 2015) in accordance with the order of the USSR Ministry of Health No. 775 of August 12, 1977.
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Sorkina, O., Yakovleva, L., Polezhaeva, T. et al. Morphofunctional state of the salivary glands of Wistar rats under prolonged exposure to alcohol. Comp Clin Pathol 31, 497–507 (2022). https://doi.org/10.1007/s00580-022-03348-y
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DOI: https://doi.org/10.1007/s00580-022-03348-y