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Insights into the mechanism underlying reproductive toxicity of gasoline fumes in male albino rat

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Abstract

Few studies have reported reproductive toxicity of gasoline fumes in animals, but none of the findings has been fully ascertained and the mechanism underlying the observed toxicity still remains unknown. Here we present insights into the potential mechanism underlying the reproductive toxicity of gasoline fumes in 72 adult male albino rats randomly assigned to six groups of 12 rats per group. Rats in group 1 (control) were exposed to distilled water while those in groups 2, 3, 4, 5, and 6 were daily exposed to gasoline fumes for 1, 3, 5, 7, and 9 h respectively for 10 weeks. Serum level of reproductive hormones (testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin); testicular oxidative stress indices; epididymal sperm health assessment (count, motility, and morphology); activities of testis membrane-bound enzymes (Ca/ATPase, Mg/ATPase, Na/K-ATPase, total ATPase); and testicular histopathology were monitored. Gasoline compositions and its residues were quantified in the testis of exposed rats using gas chromatography–mass spectrometry (GC–MS). There were significant (p < 0.05) alterations to all the reproductive hormones and oxidative stress indices and sperm health in the exposed rats. Some gasoline residues and metabolites were detected in the testis of exposed rats and, in turn, reduced the activities of the testis membrane-bound ATPase enzymes. Several degrees of histopathological lesions were seen in the testis of rats exposed to gasoline fumes. Accumulation of gasoline residues and metabolites in the testis of exposed animal could potentially result in generation of ROS capable of inducing reproductive dysfunction.

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Data associated with this current study are available from the corresponding author on demand.

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Acknowledgements

The management of Olabisi Onabanjo University and Department of Zoology and Environmental Biology granted us permission to use its facilities for this study.

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Authors and Affiliations

  1. Department of Zoology and Environmental Biology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria

    Folarin Owagboriaye, Sulaimon Aina, Titilola Salisu & Rasheed Oladunjoye

  2. Department of Environmental Management and Toxicology, College of Environmental Management, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

    Adedamola Adenekan

  3. Department of Pure and Applied Zoology, College of Bioscience, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

    Adeyinka Aladesida & Gabriel Dedeke

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  1. Folarin Owagboriaye
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  2. Sulaimon Aina
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  3. Titilola Salisu
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  4. Rasheed Oladunjoye
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  5. Adedamola Adenekan
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  6. Adeyinka Aladesida
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Contributions

Conceptualization: Folarin Owagboriaye; methodology: Folarin Owagboriaye and Gabriel Dedeke; formal analysis and investigation: Folarin Owagboriaye, Sulaimon Aina, Rasheed Oladunjoye, Titilola Salisu, and Adedamola Adenekan; writing original draft preparation: Folarin Owagboriaye; writing review and editing: all authors; resources: Folarin Owagboriaye; supervision: Gabriel Dedeke.

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Correspondence to Folarin Owagboriaye.

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Owagboriaye, F., Aina, S., Salisu, T. et al. Insights into the mechanism underlying reproductive toxicity of gasoline fumes in male albino rat. Comp Clin Pathol 31, 439–452 (2022). https://doi.org/10.1007/s00580-022-03343-3

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