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Reproductive and systemic lysozyme activities in chicken during experimental Salmonella Gallinarum infection: a comparative analysis between various egg-laying phases

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Abstract

Despite scientific advancement made in poultry farming, the problem of fowl typhoid is still persistent, discouraging significantly the economic prospects of poultry industry. New vaccines and vaccination approaches could make cost effective differences in control of such unbridled infection. Mucosal vaccination in the reproductive tract can stimulate protective localized innate immune response against fowl typhoid. The objective of the study was to investigate induction and dynamics of lysozyme activity within reproductive tract and systemic) circulation following experimental infection with Salmonella Gallinarum at various phases of egg laying viz., pre-egg laying (17–18 weeks), onset of egg-laying phase (19–20 weeks), and peak egg-laying phase (23–24 weeks). Chickens were either infected via intravenous or intravaginal route with107 CFU−1 ml of Salmonella Gallinarum. Lysozyme activity was assessed by turbidimetric method employing suspension of Micrococcus lysodeikticus. Lysozyme activity increased during egg-laying phases (both at onset and peak egg-laying phase) and was high in reproductive wash following infection. Serum lysozyme activity was not significantly different between intravenously and intravaginally infected birds. Day 7 post-infection in each trial appeared to be crucial point for intravenously infected birds, where lysozyme activity in both the bodily secretions reduced to their lowest level.The maximum activity was noted during onset of egg laying with pronounced levels in intravaginal inoculates. Immune elicitation in reproductive tract is possible and can be enhanced by injecting suitable antigen amidst different egg-laying phases including S. Gallinarum infections.

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Acknowledgements

Tha authors sincerely acknowledge the financial support received from the Department of Biotechnology, Government of India, File No: BT/PR15613/ADV/90/196/2015.

Funding

This study was supported by the financial grant received from the Department of Biotechnology, Government of India, File No: BT/PR15613/ADV/90/196/2015.

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Authors and Affiliations

  1. Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Science University, Ludhiana, Punjab, India

    Harshit Sethi, Sidhartha Deshmukh & Harmanjit Singh Banga

  2. Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Science University, Ludhiana, Punjab, India

    Paviter Kaur

Authors
  1. Harshit Sethi
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  2. Sidhartha Deshmukh
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  3. Paviter Kaur
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  4. Harmanjit Singh Banga
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Contributions

HS collected samples and performed lysozyme assay. SD: design the experimental plan and performed experiment, drafted manuscript. PK: performed lysozyme analysis and partial drafting of manuscript. HSB: performed lysozyme analysis and review the manuscript.

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Correspondence to Harshit Sethi.

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Animal experimentation approval was obtained from local institutional animal ethics committee (IAEC, GADVASU, Ludhiana). The experiment was performed as per the guidelines laid down by the institutional animal ethics committee.

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All authors agreed to the content of the article and prior consent was obtained from each authors for submission.

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The authors declare no competing interests.

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Sethi, H., Deshmukh, S., Kaur, P. et al. Reproductive and systemic lysozyme activities in chicken during experimental Salmonella Gallinarum infection: a comparative analysis between various egg-laying phases. Comp Clin Pathol 31, 391–396 (2022). https://doi.org/10.1007/s00580-022-03338-0

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