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Antiviral activity of Phoenix dactylifera extracts against herpes simplex virus type 1: an animal study

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Abstract

Herbal medicines have recently been developed for the treatment of infectious diseases. The aim of this study was to investigate the therapeutic potential of Phoenix dactylifera fruit and kernel extracts against herpes simplex virus type 1 (HSV-1) in an animal model. Five, 10, and 15% (w/w) concentrations of aqueous, ethanol, and acetone extracts of kernel and fruit of Phoenix dactylifera were used for the formulation of cream-based drugs for the treatment of HSV-1-infected mice. The clinical improvement was evaluated using lesion scoring and histopathological assessments. Our results demonstrated that there is a potent antiviral activity associated with Phoenix dactylifera extract against HSV-1 infections. Treatment with cream containing 5% Phoenix dactylifera kernel ethanol extract was observed as the most efficient therapeutic potential to treat HSV-1-infected mice. Lesion scoring assessment and histopathological analysis showed more effective and faster antiviral activity of this treatment than other extracts and concentrations in comparison with the acyclovir, as the reference drug, for the treatment of HSV-1 infections. These findings suggest that Phoenix dactylifera extract may be considered as an alternative to acyclovir and potential phototherapeutic against HSV-1 diseases; however, randomized clinical trial studies are strongly needed to ensure the quality, safety, and efficacy of the drug.

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References

  • Akram M et al (2018) Antiviral potential of medicinal plants against HIV, HSV, influenza, hepatitis, and coxsackievirus: A systematic review. Phytother Res 32:811–822

    Article  Google Scholar 

  • Andtbacka RHI et al (2016) Preliminary results from phase II study of combination treatment with HF10, a replication-competent HSV-1 oncolytic virus, and ipilimumab in patients with stage IIIb, IIIc, or IV unresectable or metastatic melanoma. J Clin Oncol 34:9543–9543

    Article  Google Scholar 

  • Benzekri R, Bouslama L, Papetti A, Hammami M, Smaoui A, Limam F (2018) Anti HSV-2 activity of Peganum harmala (L.) and isolation of the active compound. Microb Pathog 114:291–298

    Article  CAS  Google Scholar 

  • Birkmann A, Zimmermann H (2016) HSV antivirals–current and future treatment options. Curr Opin Virol 18:9–13

  • Chuanasa T et al (2008) Anti-herpes simplex virus (HSV-1) activity of oxyresveratrol derived from Thai medicinal plant: Mechanism of action and therapeutic efficacy on cutaneous HSV-1 infection in mice. Antiviral Res 80:62–70

    Article  CAS  Google Scholar 

  • Churqui MP, Lind L, Thörn K, Svensson A, Savolainen O, Aranda KT, Eriksson K (2018) Extracts of Equisetum giganteum L and Copaifera reticulate Ducke show strong antiviral activity against the sexually transmitted pathogen herpes simplex virus type 2. J Ethnopharmacol 210:192–197

    Article  Google Scholar 

  • Du Q, Gu Z, Leneva I, Jiang H, Li R, Deng L, Yang Z (2019) The antiviral activity of arbidol hydrochloride against herpes simplex virus type II (HSV-2) in a mouse model of vaginitis. Int Immunopharmacol 68:58–67

    Article  CAS  Google Scholar 

  • Guido F et al (2011) Chemical and aroma volatile compositions of date palm (Phoenix dactylifera L.) fruits at three maturation stages. Food Chem 127:1744–1754

    Article  Google Scholar 

  • Hazlett LD, Berk RS (1984). Effect of C3 depletion on experimental Pseudomonas aeruginosa ocular infection: histopathological analysis. Infect Immun, 43(3), 783-790

  • Houde L, Dumas C, Leroux T (2009) Ethics: Views from IACUC members. Altern Lab Anim 37:291–296

    Article  CAS  Google Scholar 

  • Hu B et al (2016) Cellular responses to HSV-1 infection are linked to specific types of alterations in the host transcriptome. Sci Rep 6:28075

    Article  CAS  Google Scholar 

  • Hussain MI, Farooq M, Syed QA (2020) Nutritional and biological characteristics of the date palm fruit (Phoenix dactylifera L.)–a review. Food Biosci 34:100509

  • Jassim SA, Naji MA (2010) In vitro evaluation of the antiviral activity of an extract of date palm (Phoenix dactylifera L.) pits on a Pseudomonas phage. Evid Based Complement Alternat Med 7:57–62

    Article  Google Scholar 

  • Klysik K, Pietraszek A, Karewicz A, Nowakowska M (2018) Acyclovir in the treatment of herpes viruses-a review. Curr Med Chem 27(24):4118–4137

    Article  Google Scholar 

  • Lipipun V et al (2011) Topical cream-based oxyresveratrol in the treatment of cutaneous HSV-1 infection in mice. Antiviral Res 91:154–160

    Article  CAS  Google Scholar 

  • Mazia RS et al (2016) Formulation and evaluation of a mucoadhesive thermoresponsive system containing Brazilian green propolis for the treatment of lesions caused by herpes simplex type I. J Pharm Sci 105:113–121

    Article  CAS  Google Scholar 

  • Minami M, Kita M, Nakaya T, Yamamoto T, Kuriyama H, Imanishi J (2003) The inhibitory effect of essential oils on herpes simplex virus type-1 replication in vitro. Microbiol Immunol 47:681–684

    Article  CAS  Google Scholar 

  • Moradi M-T, Rafieian-Kopaei M, Karimi A (2016) A review study on the effect of Iranian herbal medicines against in vitro replication of herpes simplex virus. Avicenna J Phytomed 6:506

    CAS  PubMed  PubMed Central  Google Scholar 

  • Nocchi SR et al (2017) Antiviral activity of crude hydroethanolic extract from Schinus terebinthifolia against herpes simplex virus type 1. Planta Med 234:509–518

    Google Scholar 

  • Petrera E, Coto CE (2009) Therapeutic effect of meliacine, an antiviral derived from Melia azedarach L., in mice genital herpetic infection. Phytother Res 23:1771–1777

    Article  CAS  Google Scholar 

  • Rechenchoski DZ et al (2020) Mangiferin: a promising natural xanthone from Mangifera indica for the control of Acyclovir–resistant herpes simplex virus 1 infection. Bioorg Medicinal Chem 28:115304

  • Rezazadeh F, Moshaverinia M, Motamedifar M, Alyaseri M (2016) Assessment of anti HSV-1 activity of Aloe vera gel extract: An in vitro study. J Dent 17:49

    Google Scholar 

  • Sharifi-Rad J, Iriti M, Setzer WN, Sharifi-Rad M, Roointan A, Salehi B (2018) Antiviral activity of Veronica persica Poir. on herpes virus infection Cell Mol Biol 64:11–17

  • Shen M-X et al (2019) Antiviral properties of R. tanguticum nanoparticles on herpes simplex virus type I in vitro and in vivo. Front Pharmacol 10:959

  • Tiwari V, Tarbutton MS, Shukla D (2015) Diversity of heparan sulfate and HSV entry: Basic understanding and treatment strategies. Molecules 20:2707–2727

    Article  Google Scholar 

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Acknowledgements

This research was supported by Medical Microbiology Research Center, Qazvin University of Medical Sciences. Also, we thank our colleagues from Qazvin University of Medical Science who assisted us in this research.

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Author notes

  1. Razzagh Mahmoudi

    Present address: Qazvin University of Medical Sciences, Bahonar Blvd., P.O. Box: 34185-754, Qazvin, Iran

Authors and Affiliations

  1. Department of Food Hygiene and Safety, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran

    Samaneh Allahyari

  2. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

    Babak Pakbin, Zahra Amani, Razzagh Mahmoudi & Amir Peymani

  3. Department of Basic Science, University of Tabriz, Tabriz, Iran

    Gholamreza Hamidiyan

  4. Health Products Safety Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

    Peyman Qajarbeygi

  5. Department of Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran

    Shaghayegh Mousavi

Authors
  1. Samaneh Allahyari
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  2. Babak Pakbin
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Corresponding author

Correspondence to Razzagh Mahmoudi.

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The animal experiment at the present study was done upon approval by the ethical committee board, Faculty of medicine, Qazvin University of Medical Science, Tehran, Iran (2016/08/10/008).

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Allahyari, S., Pakbin, B., Amani, Z. et al. Antiviral activity of Phoenix dactylifera extracts against herpes simplex virus type 1: an animal study. Comp Clin Pathol 30, 945–951 (2021). https://doi.org/10.1007/s00580-021-03293-2

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  • DOI: https://doi.org/10.1007/s00580-021-03293-2

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