Abstract
Oxidative injury is one of the mechanisms involved in thyroid dysfunctions following pesticide exposure. The present study evaluated the mitigating effect of melatonin (ML) on thyroid dysfunctions induced by gestational and lactational exposure to chlorpyriphos (CP) and cypermethrin (CY) in male rats. Six groups of 5 male rats in each group were exposed in utero and via lactation up till postnatal day (PND) 21 to the following regimens: DW, SO, and ML groups were administered distilled water (2 ml/kg), soya oil (2 ml/kg), and ML (0.5 mg/kg) while CM group was co-administered with CP (1.9 mg/kg) and CY (7.5 mg/kg). The MC group was pretreated with ML (0.5 mg/kg) followed by co-exposure to CP (1.9 mg/kg) and CY (7.5 mg/kg), 30 min later while the CM group was given CP (1.9 mg/kg) and CY (7.5 mg/kg) and then administered ML (0.5 mg/kg), 30 min later. Thereafter, sera obtained from blood samples collected at PND 91 were assayed for the concentrations of triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH). The thyroid gland (TG) was evaluated for acetylcholinesterase (AChE) activity and level of oxidative injuries by determining the levels of malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase. Alterations in TH concentrations, AChE activity, and oxidative changes to the TG in the CM group were mitigated by pre- and post-exposure administration of ML. Melatonin mitigated thyroid dysfunction and oxidative injury induced by prenatal and lactational exposure to CP and CY partly due to its antioxidant properties.
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Shittu, M., Ambali, S.F., Ayo, J.O. et al. Melatonin mitigates thyroid dysfunction induced by gestational and lactational exposure to mixture of chlorpyriphos and cypermethrin in male Wistar rats. Comp Clin Pathol 30, 529–540 (2021). https://doi.org/10.1007/s00580-021-03247-8
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DOI: https://doi.org/10.1007/s00580-021-03247-8