Abstract
Blood chemistry of growing male pigs fed low crude protein (CP) and low-energy diets supplemented with multi-enzyme was determined using 36 male pigs aged 8–10-weeks old. Two metabolizable energy (3000 and 2600 kcal ME /kg) and three protein (18, 16, and 14% CP) levels were used to formulate 6 treatment diets: T1 (3000 kcal ME/kg and 18% CP), T2 (3000 kcal ME/Kg and 16% CP), T3 (3000 kcal ME/kg and 14% CP), T4 (2600 kcal ME/kg and 18% CP), T5 (2600 kcal ME/kg and 16% CP) and T6 (2600 kcal ME/kg and 14% CP). T1 served as the control diet, while T2–T6 was supplemented with 1.0 g enzyme/kg feed each. The treatments were replicated thrice with two pigs per replicate in a 2 × 3 factorial and analysed statistically. Serum total protein, albumin, globulin, albumin/globulin ratio, urea, creatinine, urea/creatinine ratio, cholesterol, alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) were analysed. Low-energy diets (3000 and 2600 kcal ME/kg) plus enzyme supplementation did not affect (P > 0.05) any of the serum parameter measured. Also, dietary low CP level plus enzyme supplementation influenced (P < 0.05) aspects of the serum parameters. Higher (P < 0.05) total protein, albumin and creatinine were noticed in pigs fed with T2 diet; while pigs fed with T4 diet had higher (P < 0.05) urea compared with those on the other 5 treatment diets. Significant dietary interaction effects (P < 0.05) were observed on cholesterol, ALT, AST and ALP. The quadratic regression model revealed that aspects of serum parameters in pigs were optimized at different low-protein and low-energy levels. In conclusion, the results suggest that pigs on T2 diet had the best serum parameters.
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At the start of the study, the approval of FUTO bioethics committee was obtained. The veterinary staff attached to the farm examined and certified the pigs healthy at the beginning of the study.
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Amaefule, R.A., Iwuji, T.C., Ogbuewu, I.P. et al. Blood chemistry of growing male pigs in response to low protein and energy diets with EnziBlend Plus supplementation. Comp Clin Pathol 29, 1157–1164 (2020). https://doi.org/10.1007/s00580-020-03163-3
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DOI: https://doi.org/10.1007/s00580-020-03163-3