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Health performance and blood profile changes in commercial broilers supplemented with dietary monocalcium phosphate

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Abstract

Fast-growing broilers faced with various leg health problems are in crucial need of an adequate nutritional supply of both calcium and phosphorus. The aim of the current work was to study the effect of different level of monocalcium phosphate supplementation on the health performance, haematology, and biochemistry changes of commercial broiler chickens. A total of 108 broiler birds (Cobb 500) were randomly divided into three treatment groups with six replicates consisting of six birds per replicates. Treatment 1 (control) was fed with available commercial starter and finisher diets containing dicalcium phosphate. Treatments 2 and 3 were fed with the same commercial diets but supplemented with 0.5% and 1.0% of monocalcium phosphate respectively. Throughout the 42 days of feeding trial, broilers from each treatment group were observed and recorded daily for any abnormal signs of leg disorders. Six broilers were randomly selected on days 21 and 42 from each treatment group where blood samples were collected via the brachial vein into anticoagulant (EDTA) and plain blood tubes for haematology and biochemistry analysis. Leg weakness, lameness, recumbency, and splay legs were the main findings observed in broiler demonstrating leg problems. The total broiler culled because of leg problem was relatively highest in T3 (4 broilers), followed by T1 (2 broilers), and T2 (none). There were significant differences (P ˂ 0.05) in the WBC, total protein, albumin, globulin, and alkaline phosphatase on day 21. Only WBC and alkaline phosphatase exhibited significant differences (P ˂ 0.05) between groups on day 42. Broilers supplemented with 0.5% MCP (T2) revealed the highest significant values compared to the other treatment groups for both starter and finisher phases. There were no significant differences (P > 0.05) in the other parameters. In summary, 0.5% monocalcium phosphate was recommended as supplementation through this study; as it improves the overall health performances of the commercial broiler chickens.

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References

  • Abdi-Hachesoo B, Talebi A, Asri-Rezaei S (2011) Comparative study on blood profiles of indigenous and ross-308 broiler breeders. Glob Vet 7(3):238–241

    Google Scholar 

  • Ahmad T, Rasool S, Sarwar M, Haq A, Hasan Z (2000) Effect of microbial phytase produced from a fungus Aspergillus niger on bioavailability of phosphorus and calcium in broiler chickens. Anim Feed Sci Technol 83(2):103–114

    Article  CAS  Google Scholar 

  • Albokhadaim I (2012) Hematological and some biochemical values of indigenous chickens in Al-Ahsa, Saudi Arabia during summer season. Asian J Poult Sci 6(4):138–145

    Article  CAS  Google Scholar 

  • Chung ELT, Abdullah FFJ, Adamu L, Marza AD, Ibrahim HH, Zamri-Saad M, Haron AW, Saharee AA, Lila MAM, Omar AR, Bakar MZA, Norsidin MJ (2015) Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B:2 via oral and subcutaneous route of infections. Vet World 8(6):783–792

    Article  CAS  PubMed  Google Scholar 

  • Chung ELT, Kamalludin MH, Jesse FFA, Reduan MFH, Loh TC, Idrus Z (2019) Effect of monocalcium phosphate supplementation on the growth performance, carcass characteristic, gut histomorphology, meat and bone quality of broiler chickens. Pertanika J Trop Agric Sci 42(4):1237–1250

    Google Scholar 

  • Dinev I (2012) Leg weakness pathology in broiler chickens. J Poult Sci 49(2):63–67

    Article  Google Scholar 

  • Dozier WA, Davis AJ, Freeman ME, Ward TL (2003) Early growth and environmental implications of dietary zinc and copper concentrations and sources of broiler chicks. Br Poult Sci 44:726–731

    Article  CAS  Google Scholar 

  • Driver JP, Pesti GM, Bakalli RI, Edwards HM (2006) The effect of feeding calcium- and phosphorus-deficient diets to broiler chickens during the starting and growing-finishing phases on carcass quality. Poult Sci 85(11):1939–1946

    Article  CAS  Google Scholar 

  • Eeckhout W, De Paepe M (1994) Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Anim Feed Sci Technol 47(1–2):19–29

    Article  CAS  Google Scholar 

  • Fernandes JIM, Lima FR, Mendonca CX, Mabe I, Albuquerque R, Leal PM (1999) Relative bioavailability of phosphorus in feed and agricultural phosphates for poultry. Poult Sci 78(12):1729–1736

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez-Ceron F, Rekaya R, Anthony NB, Aggrey SE (2015) Genetic analysis of leg problems and growth in a random mating broiler population. Poult Sci 94(2):162–168

    Article  CAS  PubMed  Google Scholar 

  • Hamdi M, López-Verge S, Manzanilla EG, Barroeta AC, Pérez JF (2015) Effect of different levels of calcium and phosphorus and their interaction on the performance of young broilers. Poult Sci 94(9):2144–2151

    Article  CAS  PubMed  Google Scholar 

  • Han J, Wang J, Chen G, Qu H, Zhang J, Shi C, Yan Y, Cheng Y (2016) Effects of calcium to non-phytate phosphorus ratio and different sources of vitamin D on growth performance and bone mineralization in broiler chickens. R Bras Zootec 45(1):1–7

    Article  CAS  Google Scholar 

  • Hurwitz S, Plavnik I, Shapiro A, Wax E, Talpaz H, Bar A (1995) Calcium metabolism and requirements of chickens are affected by growth. J Nutr 125(10):2679–2686

    CAS  Google Scholar 

  • Knowles TG, Kestin SC, Haslam SM, Brown SN, Green LE, Butterworth A, Pope SJ, Pfeiffer D, Nicol CJ (2008) Leg disorder in broiler chickens: prevalence, risk factors and prevention. PLoS One:e1545

    Article  CAS  PubMed  Google Scholar 

  • Kral I, Suchy P (2000) Haematological studies in adolescent breeding cocks. Acta Vet Brno 69(3):189–194

    Article  Google Scholar 

  • Lentfer TL, Pendl H, Gebhardt-Henrich SG, Frohlich EKF, Von Borell E (2015) H/L ratio as a measurement of stress in laying hens – methodology and reliability. Br Poult Sci 56(2):157–163

    Article  CAS  PubMed  Google Scholar 

  • Liu LW, Su J, Luo Y (2012) Effect of partial replacement of dietary monocalcium phosphate with neutral phytase on growth performance and phosphorus digestibility in gibel carp, Carassius auratus gibelio (Bloch). Aquac Res 43(9):1404–1413

    Article  CAS  Google Scholar 

  • Liu JB, Chen DW, Adeola O (2013) Phosphorus digestibility response of broiler chickens to dietary calcium-to-phosphorus ratios. Poult Sci 92(6):1572–1578

    Article  CAS  PubMed  Google Scholar 

  • Mutucumarana RK, Ravindran V, Ravindran G, Cowieson AJ (2014) Influence of dietary calcium concentration on the digestion of nutrients along the intestinal tract of broiler chickens. J Poult Sci 51:392–401

    Article  CAS  Google Scholar 

  • Proszkowiec-Weglarz M, Angel R (2013) Calcium and phosphorus metabolism in broilers: effect of homeostatic mechanism on calcium and phosphorus digestibility. J Appl Poult Res 22(3):609–627

    Article  CAS  Google Scholar 

  • Rehman MS, Mahmud A, Mehmood S, Pasha TN, Hussain J, Khan MT (2017) Blood biochemistry and immune response in Aseel chicken under free range, semi-intensive, and confinement rearing systems. Poult Sci 96:226–233

    Article  CAS  Google Scholar 

  • Talebi A, Asri-Rezaei S, Rozeh-Chai R, Sahraei R (2005) Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbor-acres and Arian). Int J Poult Sci 4(8):573–579

    Article  Google Scholar 

  • Thiruvenkadan AK, Prabakaran R, Panneerselvam S (2011) Broiler breeding strategies over the decades: an overview. Worlds Poult Sci J 67(02):309–336

    Article  Google Scholar 

  • Virden WS, Kidd MT (2009) Physiological stress in broilers: ramifications on nutrient digestibility and responses. J Appl Poult Res 18(2):338–347

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the staff of the Department of Animal Science, Faculty of Agriculture, UPM for their technical assistance.

Funding

This study was funded by the Putra Grant-Putra Young Initiative (GP-IPM), Universiti Putra Malaysia (Grant Number 9638400).

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Authors and Affiliations

  1. Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia UPM, 43400, Serdang, Selangor, Malaysia

    Eric Lim Teik Chung, Mamat Hamidi Kamalludin, Lee Wai Ling, Nur Madihah Mahzan, Nur Nashran Mahran Mohd Ali Henipah, Teck Chwen Loh & Zulkifli Idrus

  2. Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia UPM, 43400, Serdang, Selangor, Malaysia

    Eric Lim Teik Chung, Mamat Hamidi Kamalludin, Faez Firdaus Abdullah Jesse, Teck Chwen Loh & Zulkifli Idrus

  3. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia UPM, 43400, Serdang, Selangor, Malaysia

    Faez Firdaus Abdullah Jesse

  4. Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengakalan Chepa, 16100, Kota Bharu, Kelantan, Malaysia

    Mohd Farhan Hanif Reduan

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  1. Eric Lim Teik Chung
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  2. Mamat Hamidi Kamalludin
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  9. Zulkifli Idrus
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Correspondence to Eric Lim Teik Chung.

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The content of the manuscript was read by all authors and recommended it worthy of publication.

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All the experimental procedures were conducted in accordance with the Institutional Animal Care and Use Committee of Research Policy at Universiti Putra Malaysia (UPM).

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Chung, E.L.T., Kamalludin, M.H., Jesse, F.F.A. et al. Health performance and blood profile changes in commercial broilers supplemented with dietary monocalcium phosphate. Comp Clin Pathol 29, 573–579 (2020). https://doi.org/10.1007/s00580-019-03085-9

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