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Effect of storage time on transforming growth factor beta 1 and vascular endothelial growth factor subunit A concentration in canine platelet-rich fibrin

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Abstract

Platelet-rich fibrin (PRF) has gained more interests as an alternative modality for periodontal therapy due to its anti-inflammatory effect from platelet cytokines embedded in fibrin network. One of the factors that affect efficacy of PRF is the concentration of the incorporated cytokines. However, there is inadequate information regarding PRF preservation. Thus, this study was conducted to build on the knowledge in canine study regarding cytokine concentration and also the effect of storage condition. Transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor subunit A (VEGF-A) in canine’s blood (n = 10) using advanced PRF plus technique were determined by means of enzyme-linked immunosorbent assay (ELISA) at 3 different time points; immediately (baseline), 7 days after storage at − 20 °C, and 14 days after storage at − 20 °C. Data on concentration at each time points were compared. The result revealed a pattern of time-dependent decay; TGF-β1 decreased from 171.50 ng/ml (at baseline) to 125.8 ng/ml (day 7) and 88.9 ng/ml (day 14), VEGF-A decreased from 88.21 pg/ml (at baseline) to 70.82 pg/ml (day 7) and 63.44 pg/ml (day 14). These findings indicated that PRF provided the highest cytokine concentration while used promptly after preparation.

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References

  • Albuquerque C, Morinha F, Requicha J, Martins T, Dias I, Guedes-Pinto H, Bastos E and Viegas C 2012. Canine periodontitis: The dog as an important model for periodontal studies. The Veterinary Journal. 191(3):299–305

  • Booij-Vrieling HE, van der Reijden WA, Houwers DJ, de Wit WEAJ, Bosch-Tijhof CJ, Penning LC, van Winkelhoff AJ and Hazewinkel HAW 2010. Comparison of periodontal pathogens between cats and their owners. Veterinary Microbiology. 144(1–2):147–152

  • Di Bello A, Buonavoglia A, Franchini D, Valastro C, Ventrella G, Greco M and Corrente M 2014. Periodontal disease associated with red complex bacteria in dogs. Journal of Small Animal Practice. 55(3):160–163

  • Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B (2006a) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(3):e37–e44

    Article  Google Scholar 

  • Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B (2006b) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(3):e45–e50

    Article  Google Scholar 

  • Dohan Ehrenfest DM, Del Corso M, Diss A, Mouhyi J, Charrier J-B (2009) Three-dimensional architecture and cell composition of a Choukroun’s platelet-rich fibrin clot and membrane. J Periodontol 81(4):546–555

    Article  Google Scholar 

  • El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A, Booms P, Dohle E, Sader R, Kirkpatrick CJ, Choukroun J, Ghanaati S (2017) Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept). Eur J Trauma Emerg Surg

  • Fujioka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang Y, Choukroun J (2016) Optimized platelet-rich fibrin with the low-speed concept: growth factor release, biocompatibility, and cellular response. J Periodontol 88(1):112–121

    Article  Google Scholar 

  • Hatakeyama I, Marukawa E, Takahashi Y, Omura K (2014) Effects of platelet-poor plasma, platelet-rich plasma, and platelet-rich fibrin on healing of extraction sockets with buccal dehiscence in dogs. Tissue Eng A 20(3–4):874–882

    CAS  Google Scholar 

  • He L, Lin Y, Hu X, Zhang Y, Wu H (2009) A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108(5):707–713

    Article  Google Scholar 

  • Kaigler D, Cirelli JA, Giannobile WV (2006) Growth factor delivery for oral and periodontal tissue engineering. Expert opinion on drug delivery 3(5):647–662

    Article  CAS  Google Scholar 

  • Kobayashi E, Flückiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ (2016) Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 20(9):2353–2360

    Article  Google Scholar 

  • Lacci KM, Dardik A (2010) Platelet-rich plasma: support for its use in wound healing. Yale J Biol Med 83(1):1–9

    CAS  PubMed  PubMed Central  Google Scholar 

  • Li Q, Pan S, Dangaria SJ, Gopinathan G, Kolokythas A, Chu S, Geng Y, Zhou Y, Luan X (2013) Platelet-rich fibrin promotes periodontal regeneration and enhances alveolar bone augmentation. Biomed Res Int 2013:13

    Google Scholar 

  • Masako FK, MR J, Maria H, Umadevi K, Yufeng Z, Joseph C (2017) Optimized platelet-rich fibrin with the low-speed concept: growth factor release, biocompatibility, and cellular response. J Periodontol 88(1):112–121

    Article  Google Scholar 

  • Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, Nakata K, Uematsu K, Su C-Y, Kawase T (2016) Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2(1):19

    Article  Google Scholar 

  • Naik B, Karunakar P, Jayadev M, Marshal V (2013) Role of platelet rich fibrin in wound healing: a critical review. J Conserv Dent 16(4):284–293

    Article  CAS  Google Scholar 

  • Niemiec BA 2008. Periodontal Disease. Topics in Companion Animal Medicine. 23(2):72–80

  • Pakyari M, Farrokhi A, Maharlooei MK, Ghahary A (2013) Critical role of transforming growth factor beta in different phases of wound healing. Advances in Wound Care 2(5):215–224

    Article  Google Scholar 

  • Pieri FA, Daibert APF, Bourguignon E and Moreira MAS 2012. Periodontal disease in dogs. In: INTECH Open Access Publisher.

  • Preeja C, Arun S (2014) Platelet-rich fibrin: its role in periodontal regeneration. Saudi J Dent Res 5(2):117–122

    Article  Google Scholar 

  • Silva R, Carmona J and Rezende C 2012. Comparison of the effect of calcium gluconate and batroxobin on the release of transforming growth factor beta 1 in canine platelet concentrates. Vol 8. In. 121

  • Stephan B, Olivera S, GM S, Harold B, Marjana TC (2008) PERSPECTIVE ARTICLE: Growth factors and cytokines in wound healing. Wound Repair Regen 16(5):585–601

    Article  Google Scholar 

  • Su CY, Kuo YP, Tseng YH, Su C-H, Burnouf T (2009) In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol 108(1):56–61

    Article  Google Scholar 

  • Suchetha A, Lakshmi P, Bhat D, Mundinamane DB, Soorya KV, Bharwani GA (2015) Platelet concentration in platelet concentrates and periodontal regeneration-unscrambling the ambiguity. Contemp Clin Dent 6(4):510–516

    Article  CAS  Google Scholar 

  • Wang Z, Wang Z, Lu WW, Zhen W, Yang D, Peng S (2017) Novel biomaterial strategies for controlled growth factor delivery for biomedical applications. Npg Asia Materials 9:e435

    Article  CAS  Google Scholar 

  • Watanabe T, Isobe K, Suzuki T, Kawabata H, Nakamura M, Tsukioka T, Okudera T, Okudera H, Uematsu K, Okuda K, Nakata K, Kawase T (2017) An evaluation of the accuracy of the subtraction method used for determining platelet counts in advanced platelet-rich fibrin and concentrated growth factor preparations. Dent J 5(1):7

    Article  Google Scholar 

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Acknowledgments

Authors would like to express our sincere appreciation to Ms. Siripen Komolvanich, Mr. Kritsada Thongmeesee, Ms. Jutamas Yongyai, Ms. Napapond Chanyathanapa, and Ms. Aujchariyaporn Phongphuwanun for the laboratory technical support.

Funding

This research was financially supported by the 90th Anniversary of Chulalongkorn University and the Graduate School, Chulalongkorn University to commemorate the 72nd anniversary of His Majesty King Bhumibol Adulyadej.

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Authors and Affiliations

  1. Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand

    C. Kornsuthisopon & C. Kalpravidh

  2. Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    S. Techangamsuwan & N. Pirarat

  3. Veterinary Diagnostic Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    P. Makhum

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  1. C. Kornsuthisopon
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  2. S. Techangamsuwan
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  3. P. Makhum
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  4. C. Kalpravidh
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  5. N. Pirarat
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Correspondence to N. Pirarat.

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Kornsuthisopon, C., Techangamsuwan, S., Makhum, P. et al. Effect of storage time on transforming growth factor beta 1 and vascular endothelial growth factor subunit A concentration in canine platelet-rich fibrin. Comp Clin Pathol 28, 1805–1810 (2019). https://doi.org/10.1007/s00580-019-03003-z

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  • DOI: https://doi.org/10.1007/s00580-019-03003-z

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