DGGR-lipase for effective diagnosis of pancreatitis in horses
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Equine pancreatitis has no reliable ante-mortem test and is considered rare. The 1,2-o-dilauryl-rac-glycero-3-glutarate-(6′-methylresorufin) ester (DGGR) lipase assay is specific for pancreatic lipase (PL). It was developed for humans and validated in dogs, cats and rats as specific for pancreatitis diagnosis. We used a novel validation strategy to confirm this tissue specificity extended to horses and determine reference ranges and pancreatitis prevalence. One hundred thirty-nine horses were used. Biopsies were collected from 3 healthy cadavers and venous blood from 109 clinical cases and 27 training racehorses. DGGR-lipase was demonstrated to only occur significantly in the pancreas using 7 different tissues collected at euthanasia that were cryopreserved and assayed for PL and 6, other, routine biomarkers (amylase, CK, GLD, AST, ALT, ALP). PL range was determined in healthy horses and in pancreatitis cases. PL is an inexpensive, highly-effective biomarker with the highest tissue activity and specificity of routine biomarkers. Equine pancreatitis occurs with a high prevalence and severity similar to that in dogs and cats, and in association with a wide variety of gastrointestinal disorders and with training racehorses. PL should be routinely assayed in horses with gastrointestinal disorders and monitored in training racehorses.
KeywordsEquine Pancreatitis Pancreatic lipase Diagnostic test
Right dorsal displacement of the large colon
Compliance with ethical standards
For this type of study formal consent is not required.
Conflict of interest
The authors declare that they have no conflict of interest.
- Dehghani SN, Tadjalli M, Seifali A (2005) Sialography in horse: technique and normal appearance. Vet Archiv 75:531–540Google Scholar
- Embleton JK, Poutonb CW (1997) Clin Biochem 38:365–370. Structure and function of gastro-intestinal lipases. Adv Drug Deliv Rev 25:15–32Google Scholar
- Higginson CM (1937) Pancreatitis of the horse. Can J Comp Med 1:27–36Google Scholar
- Lilley CW, Beeman GM (1981) Gastric dilatation associated with acute necrotizing pancreatitis. Eq Pract 3:8–15Google Scholar
- Lohman KL, Allen AL (2015) Chronic active interstitial pancreatitis as a cause of transverse colonic obstruction and colic in a horse. Can Vet J 56:1177–1180Google Scholar
- O’Brien PJ, Landt Y, Ladenson JH (1997) Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 43:2333–2338Google Scholar
- O’Brien PJ, Papakonstantinou S, McGrath C (2014) Pancreatic, DGGR-lipase, enzymatic assay is a highly sensitive, specific and inexpensive biomarker of pancreatitis in cats and dogs. Vet Clin Pathol 42:E20–E48Google Scholar
- O'Brien PJ, Shen H, McCutcheon LJ, O’Grady M, Byrne PJ, Ferguson HW, Mirsalimi MS, Julian RJ, Sargeant JM, Tremblay RR (1992) Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: use in various animals indicates functional role of myohemoproteins. Mol Cell Biochem 112:45–52CrossRefGoogle Scholar
- O'Brien PJ, Dameron GW, Beck ML et al (1997) Cardiac troponin T is a sensitive, specific biomarker of cardiac injury in laboratory animals. Comp Med 47:486–495Google Scholar
- Prinzing U, Zielenski R, Schellong L et al (1998) Kinetic colorimetric assay for pancreatic lipase (PL) based on a chromogenic substrate. Clin Chem 44:122Google Scholar
- Tietz NW, Shuey FS (1993) Lipase in serum - the elusive enzyme: an overview. Clin Chem 39:746–756Google Scholar
- Zakaria Z, O’Brien PJ, O’Brien MF et al (2015) The DNA-alkylating, anticancer-drug temozolomide protects rats from dose-limiting pancreatic toxicity produced by the SMAC-mimetic, anticancer-drug TL3271. Vet Clin Pathol 44:E15Google Scholar