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DGGR-lipase for effective diagnosis of pancreatitis in horses

  • Jessica P. Johnson
  • John D. Stack
  • Charlotte L. McGivney
  • Michael P. Garrett
  • Peter J. O’BrienEmail author
Original Article
  • 19 Downloads

Abstract

Equine pancreatitis has no reliable ante-mortem test and is considered rare. The 1,2-o-dilauryl-rac-glycero-3-glutarate-(6′-methylresorufin) ester (DGGR) lipase assay is specific for pancreatic lipase (PL). It was developed for humans and validated in dogs, cats and rats as specific for pancreatitis diagnosis. We used a novel validation strategy to confirm this tissue specificity extended to horses and determine reference ranges and pancreatitis prevalence. One hundred thirty-nine horses were used. Biopsies were collected from 3 healthy cadavers and venous blood from 109 clinical cases and 27 training racehorses. DGGR-lipase was demonstrated to only occur significantly in the pancreas using 7 different tissues collected at euthanasia that were cryopreserved and assayed for PL and 6, other, routine biomarkers (amylase, CK, GLD, AST, ALT, ALP). PL range was determined in healthy horses and in pancreatitis cases. PL is an inexpensive, highly-effective biomarker with the highest tissue activity and specificity of routine biomarkers. Equine pancreatitis occurs with a high prevalence and severity similar to that in dogs and cats, and in association with a wide variety of gastrointestinal disorders and with training racehorses. PL should be routinely assayed in horses with gastrointestinal disorders and monitored in training racehorses.

Keywords

Equine Pancreatitis Pancreatic lipase Diagnostic test 

Abbreviations

ALP

Alkaline phosphatase

ALT

Alanine aminotransferase

AMY

Amylase

AST

Asparate aminotransferase

CK

Creatine kinase

Crea

Creatinine

DGGR

1,2-o-dilauryl-rac-glycero-3-glutarate-(6′-methylresorufin) ester

GIT

Gastrointestinal tract

GLD

Glutamate dehydrogenase

Hb

Haemoglobin

PL

Pancreatic lipase

RDD

Right dorsal displacement of the large colon

Notes

Compliance with ethical standards

Ethical approval

For this type of study formal consent is not required.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Large Animal SurgeryUniversity College Dublin Veterinary HospitalDublin 4Ireland
  2. 2.Department of Clinical PathologyUniversity College Dublin Veterinary HospitalDublin 4Ireland

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