The beneficial effect of equine chorionic gonadotropin hormone (eCG) on the in vitro co-culture of bovine spermatogonial stem cell with Sertoli cells

  • Arezoo Kashfi
  • Reza Narenji SaniEmail author
  • Mahmood Ahmadi-hamedani
Original Article


Spermatogenesis is a complex developmental process that originates from spermatogonia stem cell. This process consists of sequential, highly organized steps of cell proliferation and differentiation resulting in the generation of functional spermatozoa. Many growth factors, hormones, and cell interactions of germ cells with Sertoli cells regulate these processes, and the failure of any of the processes can lead to male infertility The aim of the present study was to determine the effects of equine chorionic gonadotropin on spermatogonial stem cell colony formation and differentiation after in vitro co-culture with Sertoli cells. In this experimental study, Sertoli and spermatogonial stem cells were isolated from 3- to 5-month-old calves. Co-cultured Sertoli and spermatogonial stem cells were treated with equine chorionic gonadotropin in treatment groups before colony assay. The present study showed that equine chorionic gonadotropin increase colony number and decrease colony diameter. Equine chorionic gonadotropin can induce proliferative pathway and inhibit differentiation process in spermatogonia stem cell co-culture with Sertoli cells.


Bovine Co-culture eCG Sertoli cells Spermatogonia stem cells 


Funding information

This work was supported by grants to the second author from the Semnan University for DVM thesis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The research was conducted in accordance the National Health guidelines, USA, and were approved by the Animal Research Ethical Committee of Semnan University of Faculty of veterinary science.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Veterinary ScienceSemnan UniversitySemnanIran
  2. 2.Department of Clinical Sciences, Faculty of Veterinary MedicineSemnan UniversitySemnanIran

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