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Comparative Clinical Pathology

, Volume 28, Issue 4, pp 1077–1085 | Cite as

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter

  • Hori GhaneialvarEmail author
  • Abbas Sahebghadam Lotfi
  • Sareh Arjmand
  • Leila Soltani
  • Azra Kenarkoohi
  • Mohammad Mahdi Zangeneh
Original Article
  • 69 Downloads

Abstract

Due to their unique biological characteristics, mesenchymal stem cells (MSCs) are useful in developing cell-based therapies, tissue regeneration, and many other applications of cellular and molecular genetics. Use of lentiviral system allows the integration of specific nucleic acid sequence into a target cell genome near an active transcription site in such a way that it is stable and heritable. The aim of the present study was to evaluate the gene transduction efficiency on the cells of different species using lentivirus along with cytomegalovirus in a lentiviral shuttle vector. In this study, the third-passage isolated MSCs from four different species, including ovine and goat cardiac fibroblasts, HEK293T, and L929 cell line were transduced by concentrated virus particles. Then, transduction percentage was detected by flow cytometry. The findings showed different transduction efficiencies for various cells of one species or other species, indicating that differentiated and undifferentiated cells had different transduction percentages. Different degrees of transduction observed in differentiated and undifferentiated cells might be the result of various lentiviral receptors on the plasma membrane, site of integration, and activity of CMV promoter. Further, other regulatory factors involved in cell transduction and target gene expression can cause different percentages of transduction.

Keywords

Mesenchymal stem cells Cell therapy Lentiviral vectors Gene therapy Transfection 

Notes

Acknowledgments

We sincerely thank Mr. Jedi, the manager of Rock slaughterhouse for his generous support of providing the samples.

Funding

The authors would like to thank the Ilam University of Medical Science for the financial support.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of human and laboratory animals were followed.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Biotechnology and Medicinal Plants Research Center, Faculty of MedicineIlam University of Medical SciencesIlamIran
  2. 2.Department of Clinical Biochemistry, Faculty of MedicineIlam University of Medical SciencesIlamIran
  3. 3.Department of Clinical Biochemistry, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  4. 4.Protein Research CenterShahid Beheshti UniversityTehranIran
  5. 5.Department of Animal Science, College of AgricultureIsfahan University of TechnologyIsfahanIran
  6. 6.Department of Microbiology, Faculty of MedicineIlam University of Medical SciencesIlamIran
  7. 7.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran

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