Abstract
The aim of this study was to compare phylogenic groups and virulence genes profiles of Escherichia coli isolated from poultry suffering from Colibacillosis with E.coli in the intestinal flora of healthy poultry. For this purpose, var, tsh, iucd, papc, irp2, and iss virulence genes of E. coli were investigated by multiplex PCR in 70 isolates (35 obtained from healthy samples and the other 35 isolated were attained form those with Colibacillosis) in Ardabil province, northwest of Iran. The phylogenic groups were also determined through use of PCR multiplex. Among them, 32 isolates (91%) of samples taken from poultry with Colibacillosis and 27 isolates (77%) from healthy samples contained papc gene. The results for iucd, var, and irp2 genes were 17 healthy and 20 diseased isolates, 23 healthy and 28 diseased isolates, and 32 healthy and 29 diseased isolates, respectively. In the case of tsh gene, only one isolate from healthy samples included the mentioned gene; while iss gene was observed in none of the isolates. In terms of phylogeny groups, except one, all the isolates belonged to the phylogenic group D. Results of present research revealed that the frequency of virulence genes and the diversity of phylogeny groups in isolates from healthy and diseased samples are not significantly different in the studied area.
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Arabi S, Jafarpour M, Mirinargesi M, Asl SB, Naghshbandi R, Shabanpour M (2013) Molecular characterization of avian pathogenic Escherichia coli in broilers bred in northern Iran. Glob Vet 10:382–386. https://doi.org/10.5829/idosi.gv.2013.10.4.72117
Clermont O, Bonacorsi S, Bingen E (2000a) Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66:4555–4558. https://doi.org/10.1128/AEM.66.10.4555-4558.2000
Clermont O, Bonacorsi S, Bingen E, Bonacorsi P (2000b) Rapid and simple determination of the Escherichia coli phylogenetic group rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66:4555–4558. https://doi.org/10.1128/AEM.66.10.4555-4558.2000.Updated
Clermont O, Christenson JK, Denamur E, Gordon DM (2013) The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ Microbiol Rep 5:58–65. https://doi.org/10.1111/1758-2229.12019
de Campos TA, Lago JC, Nakazato G, Stehling EG, Brocchi M, de Castro AFP, da Silveira WD (2008) Occurrence of virulence-related sequences and phylogenetic analysis of commensal and pathogenic avian Escherichia coli strains (APEC). Pesqui Veterinária Bras 28:533–540. https://doi.org/10.1590/S0100-736X2008001000015
Dziva F, Stevens MP (2008) Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenic Escherichia coli in their natural hosts. Avian Pathol 37:355–366. https://doi.org/10.1080/03079450802216652
Ewers C, Janßen T, Kießling S, Philipp HC, Wieler LH (2004) Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry. Vet Microbiol 104:91–101. https://doi.org/10.1016/j.vetmic.2004.09.008
Ewers C, Janssen T, Kiessling S, Philipp HC, Wieler LH (2005) Rapid detection of virulence-associated genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. Avian Dis 49:269–273. https://doi.org/10.1637/7293-102604R
Ghanbarpour R, Sami M, Salehi M, Ouromiei M (2011) Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran. Trop Anim Health Prod 43:153–157. https://doi.org/10.1007/s11250-010-9667-2
Gordon DM (2010) Strain typing and the ecological structure of Escherichia coli. J AOAC Int:974–984
Hassani B, Shayegh J, Ameghi A, Mikaili P, Mahmmudzadeh M (2013) Phylogenic typing of Escherichia coli isolated from broilers with collibacillosis in Tabriz, North West of Iran. Arch. Razi Inst 68:43–46
Hiki M, Usui M, Akiyama T, Kawanishi M, Tsuyuki M, Imamura S, Sekiguchi H, Kojima A, Asai T (2014) Phylogenetic grouping, epidemiological typing, analysis of virulence genes, and antimicrobial susceptibility of Escherichia coli isolated from healthy broilers in Japan. Ir Vet J 67:14. https://doi.org/10.1186/2046-0481-67-14
Jansen T (2001) Virulence-associated genes in avian pathogenic (APEC) isolated from internal organs of poultry having died from colibacillosis. Int J Med Microbiol 291:371–378. https://doi.org/10.1078/1438-4221-00143
Johnson JR, Stell AL (2000) Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 181:261–272. https://doi.org/10.1086/315217
Paixão AC, Ferreira AC, Fontes M, Themudo P, Albuquerque T, Soares MC, Fevereiro M, Martins L, Corrêa de Sá MI (2016) Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates. Poult Sci 95:1646–1652. https://doi.org/10.3382/ps/pew087
Picard B, Garcia JS, Gouriou S, Duriez P, Brahimi N, Bingen E, Elion J, Denamur E (1999) The link between phylogeny and virulence in Escherichia coli extraintestinal infection? Infect Immun 67:546–553
Qabajah M, Awwad E, Ashhab Y (2014) Molecular characterisation of Escherichia coli from dead broiler chickens with signs of colibacillosis and ready-to-market chicken meat in the West Bank. Br Poult Sci 55:442–451. https://doi.org/10.1080/00071668.2014.935998
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This paper is derived from the thesis of the first author, and the Department of Animal Science in Islamic Azad University of Shabestar is highly appreciated.
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Zakariazadeh, N., Shayegh, J. & Ghorbani, A. Phylogenetic typing and virulence gene profile of pathogenic and commensal avian Escherichia coli in Iran: a notable finding. Comp Clin Pathol 28, 525–530 (2019). https://doi.org/10.1007/s00580-019-02921-2
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DOI: https://doi.org/10.1007/s00580-019-02921-2