Effect of zinc on erythrocyte osmotic fragility and hemogram following chronic exposure to glyphosate-based herbicide in Wistar rats


The study was conducted to assess the effect of zinc on erythrocyte osmotic fragility and hemogram of rats following chronic oral exposure to glyphosate-based herbicide (BUSHFIRE®) at daily doses of 14.4–750 mg/kg of glyphosate. The erythrocyte osmotic fragility, estimated as percentage hemolysis in hypotonic saline, was significantly (p < 0.05) increased in glyphosate-exposed groups at 0.5–0.9% NaCl concentrations, and the fragility was ameliorated in the groups with zinc pretreatments only at 0.5% NaCl. The toxicity did not have any significant effect on the packed cell volume and other erythrocyte parameters. There was significant (p < 0.05) decrease in total white blood cell and lymphocyte counts in glyphosate-exposed groups, which was absent in zinc-pretreated groups. In conclusion, increased erythrocyte osmotic fragility induced by glyphosate could be prevented following zinc pretreatment, but the altered leukogram caused by the toxic action was not fully ameliorated in the pretreated group.

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We hereby acknowledge the contribution of Dr. Uchendu Chidiebere, Department of Veterinary Pharmacology, Physiology and Biochemistry, University of Jos who helped us with the data analysis.

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Correspondence to Emmanuel Vandi Tizhe.

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This work was carried out after approval was obtained from the ethical committee on the care and use of animals in research of Ahmadu Bello University, Zaria, Nigeria.

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Tizhe, E.V., Ibrahim, N.D., Fatihu, M.Y. et al. Effect of zinc on erythrocyte osmotic fragility and hemogram following chronic exposure to glyphosate-based herbicide in Wistar rats. Comp Clin Pathol 28, 1275–1279 (2019). https://doi.org/10.1007/s00580-019-02917-y

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  • Glyphosate
  • Zinc
  • Erythrocyte osmotic fragility
  • Leukogram
  • Toxicity