Comparative Clinical Pathology

, Volume 28, Issue 2, pp 349–357 | Cite as

Dietary flaxseed oil inhibits kidney NF-kappa B activation and pro-inflammatory cytokine expression in cisplatin-treated rats

  • Hend Samy Kheira
  • Shimaa Abd El-Salam El-Sayed
  • Gehad Ramadan Elsayed
  • Mohamed Abdo RizkEmail author
Original Article


The current study explores the anti-inflammatory effect of flaxseed oil (FXO) on cisplatin (CP)-induced renal damage. Rats prefed with normal experimental diets (normal/FXO diet) for 10 days were administered 30 mg kg−1 and 50 mg kg−1 i.p. CP in a single dose, while still on the experimental diets. Significant improvement in kidney function test and antioxidant enzyme activities in rats treated with combination therapy (FXO + CP) was detected. Interestingly, treatment by FXO resulted in significant downregulation (P < 0.05) in interleukin (IL)-6 and IL-1β expression in the renal tissue in comparison with CP-treated rats. Moreover, administration of FXO yielding negative and mild positive brown immunostain for tumor necrosis factor-alpha (TNF-α) or mild and moderate positive brown immunostain for nuclear factor Kappa B (NF-kB) in the renal tissue of rats treated with 3 mg kg−1 CP + FXO and 5 mg kg−1 CP + FXO, respectively. The obtained results highlight the ameliorative anti-inflammatory effect of FXO in the renal damage associated with CP administration and confirm the necessity and usefulness of FXO dietary supplement when using CP in the treatment of cancer patients.


Cisplatin Flaxseed oil Nephrotoxicity Pro-inflammatory cytokines 


Author contributions

Conceived and designed the experiments: HSK, SAE, GRE. Performed the experiments: HSK, SAE. Analyzed the data: MAR. Wrote and revised the paper: HSK, SAE, GRE, MAR.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

580_2018_2871_MOESM1_ESM.pptx (784 kb)
Figure S1 Fold change in β-actin gene expression in RT-PCR showing the effect of FXO on kidney with/without CP treatment (PPTX 783 kb)
580_2018_2871_MOESM2_ESM.pptx (755 kb)
Figure S2 Fold change in IL-6 gene expression in RT-PCR showing the effect of FXO on kidney with/without CP treatment (PPTX 755 kb)
580_2018_2871_MOESM3_ESM.pptx (798 kb)
Figure S3 Fold change in IL-1β gene expression in RT-PCR showing the effect of FXO on kidney with/without CP treatment. (PPTX 797 kb)


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  2. 2.Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt

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