Comparative Clinical Pathology

, Volume 28, Issue 2, pp 557–565 | Cite as

Rumex alveollatus hydroalcoholic extract protects CCL4-induced hepatotoxicity in mice

  • Leila Naseri
  • Mozafar Khazaei
  • Elham Ghanbari
  • Mohsen Akbari BazmEmail author
Original Article


Some plants have high antioxidant properties that can improve the damage to the tissues of the body. Rumex alveollatus L. is proposed to contain flavonoids, a group of antioxidants that exert their protective effects against free radicals. Carbon tetrachloride (CCl4) is a factor that damages the liver. This study was carried out to explore the protective effects of Rumex alveollatus L. on the liver damage induced by CCl4 in mice. In this experimental study, 30 male mice (35 ± 4) were divided into five groups, each with six mice. The negative control group was administered 0.5-ml olive oil; positive control group was administered 2-ml/kg CCl4 plus olive oil at 1:1 ratio for 4 days, and the experimental groups were administered 2 ml/kg CCl4 and Rumex alveollatus L. extract at 150, 300, and 450 mg/kg for 4 days. All administrations were done intraperitoneally. Twenty days later, blood samples were taken from the heart, and liver serum enzymes (ALT, AST, and ALP) were measured. After sacrificing the samples, liver samples were fixed in formalin to prepare the histological sections. Then, factors such as liver volume, hepatocytes, sinusoids, central veins, portal vein, bile duct, and hepatic artery were measured. Rumex alveollatus L. extract (450 mg/kg) reduced the serum level of all three liver enzymes (p < 0.05). At this dose, the volume of sinusoids, hepatocytes, and bile duct decreased significantly (p < 0.05), but the volume of portal vein and central vein increased significantly (p < 0.05). Rumex alveollatus L. extract has protective effects against CCl4-induced damages in the liver.


Rumex alveollatus L. Carbon tetrachloride (CCl4Stereology 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All institutional and national standards for the care and use of laboratory animals were followed.This article does not contain any studies with human participants performed by any of the authors.


  1. Aghel N, Rashidi I, Mombeini A (2010) Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iran J Pharm Res 285–90Google Scholar
  2. Amin A, Hamza AA (2005) Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci 77:266–278CrossRefGoogle Scholar
  3. Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203CrossRefGoogle Scholar
  4. Cantwell SL (2001) Ferret, rabbit, and rodent anesthesia. Veterinary clinics of North America exotic animal practice 4:169–191CrossRefGoogle Scholar
  5. Cetinkaya O, Siliğ Y, Cetinkaya S, Demirezer LO (2002) The effects of Rumex patientia extract on rat liver and erythrocyte antioxidant enzyme system. Die Pharmazie 57:487–488Google Scholar
  6. Chatila R (2000) Hepatotoxicity: the adverse effects of drugs and other chemicals on the liver. J Clin Gastroenterol 31:185CrossRefGoogle Scholar
  7. Feyzmahdavia M, Khazaeia M, Gholaminb B, Abbasabadia Z (2017) Protective effect of Trogopogon Graminifolius hydroalcoholic extract against acute ethanol induced liver damages in rat. JRPS 6:161–166Google Scholar
  8. Ganaie MA, Khan TH, Siddiqui NA, Ansari MN (2015) Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. Pharm Biol 53:1163–1167CrossRefGoogle Scholar
  9. Ghanbari E, Khazaei M, Yousefzaei F (2017) The restorative effect of prosopis farcta on fertility parameters and antioxidant status in diabetic rats. JBUMS 19:53–60Google Scholar
  10. Goodarzi N, Doorgard E, Pournaghi P (2018) The ameliorative potential of Sophora alopecuroides essential oil on CCl4-induced hepatotoxicity in mice; a stereological study. RJP 5:47–54Google Scholar
  11. Gundersen HJ, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc Ultrastruct 147:229–263CrossRefGoogle Scholar
  12. Gundersen HJ, BENDTSEN TF, KORBO L, MARCUSSEN N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis 96:379–394CrossRefGoogle Scholar
  13. Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719–1726CrossRefGoogle Scholar
  14. Halliwell B (1997) Antioxidants in disease mechanisms and therapy. Adv Pharmacol Sci 38:3–17CrossRefGoogle Scholar
  15. Jaeschke H, Gores GJ, Cederbaum AI, Hinson JA, Pessayre D, Lemasters JJ (2002) Mechanisms of hepatotoxicity. Toxicol Sci 65:166–176CrossRefGoogle Scholar
  16. Jimoh FO, Adedapo AA, Aliero AA, Afolayan AJ (2008) Polyphenolic contents and biological activities of Rumex ecklonianus. Pharm Biol 46:333–340CrossRefGoogle Scholar
  17. Kasote DM, Katyare SS, Hegde MV, Bae H (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 11:982–991CrossRefGoogle Scholar
  18. Khazaei M, Roshankhah S, Ghorbani R, Chobsaz F (2011) Sildenafil effect on nitric oxide secretion by normal human endometrial epithelial cells cultured in vitro. Int J Fertil & Steril 5:142Google Scholar
  19. Korkorian N, Mohammadi-Sichani M (2017) Anti-quorum sensing and antibacterial activity of Rumex alveolatus. Zahedan J Res Med Sci 19(11)Google Scholar
  20. Latha S, Chaudhary S, Ray RS (2017) Hydroalcoholic extract of Stevia rebaudiana bert. leaves and stevioside ameliorates lipopolysaccharide induced acute liver injury in rats. Biomed Pharmacother 95:1040–1050CrossRefGoogle Scholar
  21. Lee SS, Kim DH, Yim DS, Lee SY (2007) Anti-inflammatory, analgesic and hepatoprotective effect of semen of Rumex crispus. Korean J Physiol PharmacolGoogle Scholar
  22. Mirhosseini M (2017) Medicinal plants with anti-poisoning toxicity of carbon tetrachloride: an overview of the most important medicinal plants native to Iran with anti-carbon tetrachloride toxicity. Journal of Global Pharma Technology 17–20Google Scholar
  23. Nejad ZS, Bamakan AM, Sharfififar F, Mokhtari S, Iranpour M (2015) Evaluation of the hepatoprotective effects of rumex acetosella on the carbon tetrachloride-induced hepatotoxicity in rats. Avicenna J Phytomed 5:134–135CrossRefGoogle Scholar
  24. NK AT, Londonkar RL, Nayaka HB (2015) Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius. L. Biol Res 48:19CrossRefGoogle Scholar
  25. Nyengaard JR (1999) Stereologic methods and their application in kidney research. Clin J Am Soc Nephrol 10:1100–1123Google Scholar
  26. Rajkapoor B, Anandan R, Jayakar B (2002) Hepatoprotective activity of Nigella sativa Linn. Indian Drugs 39:398–399Google Scholar
  27. Saleem M, Ahmed B, Karim M, Ahmed S, Ahmad M, Qadir MI (2014) Hepatoprotective effect of aqeous methanolic extract of Rumex dentatus in paracetamol induced hepatotoxicity in mice. Bangladesh J Pharmacol 9:284–289Google Scholar
  28. Saleh NA, El-Hadidi MN, Arafa RF (1993) Flavonoids and anthraquinones of some Egyptian Rumex species (Polygonaceae). Biochem Syst Ecol 21:301–303CrossRefGoogle Scholar
  29. Sharma B, Kumar SU (2010) Hepatoprotective activity of some indigenous plants. Int J Pharmtech Res 2:568–572Google Scholar
  30. Silig Y, Cetinkaya O, Demirezer LO (2004) Effects of Rumex patientia L extract on some drug-metabolizing enzymes in rat liver, 41: 45–47Google Scholar
  31. Tavafi M, Ahmadvand H, Tamjidipoor A, Delfan B, Khalatbari AR (2011) Satureja khozestanica essential oil ameliorates progression of diabetic nephropathy in uninephrectomized diabetic rats. Tissue Cell 43:45–51CrossRefGoogle Scholar
  32. Ubhenin AE, Igbe I, Adamude FA, Falodun A (2016) Hepatoprotective effects of ethanol extract of Caesalpinia bonduc against carbon tetrachloride induced hepatotoxicity in albino rats. J Appl Sci Environ Manag 20:396–401Google Scholar
  33. Vasas A, Orbán-Gyapai O, Hohmann J (2015) The genus Rumex: review of traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 175:198–228CrossRefGoogle Scholar
  34. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018a) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl 4-induced hepatic injury in mice. Comp Clin Pathol, 1–7Google Scholar
  35. Zangeneh MM, Zangeneh A, Almasi M, Tahvilian R, Hosseini F, Moradi R (2018b) A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice. Comp Clin Pathol, 1–9Google Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Anatomical Sciences, Medical SchoolKermanshah University of Medical SciencesKermanshahIran
  2. 2.Anatomical Sciences, Fertility and Infertility Research CenterKermanshah University of Medical SciencesKermanshahIran

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