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Comparative study on the interaction of eugenol, Butylated hydroxylanisole, and Butylated hydroxyl toluene with some crucial enzymes linked to erectile dysfunction

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Abstract

This study investigated and compared the effect of natural phenolic compound (eugenol) and synthetic phenolic compounds [butylated hydroxylanisole (BHA) and butylated hydroxyl toluene (BHT)] on some crucial enzymes (angiotensin-1-converting enzyme (ACE) and arginase) linked to erectile dysfunction (ED) in vitro. Furthermore, the effects of the compounds were assessed on several antioxidant parameters including DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) free radical scavenging ability, ferric reducing power, and Fe2+ chelating ability. Ten millimolar each of the phenolic compounds was prepared in a mixture of ethanol and water (1:1 v/v), and their interactions with ACE and arginase was assessed. Furthermore, ferric-reducing antioxidant property (FRAP), Fe2+-chelating ability, DPPH, and ABTS radicals scavenging abilities were determined. The results revealed that eugenol, BHT, and BHA inhibited ACE and arginase activities in dose-dependent manner. However, eugenol showed the highest inhibitory effect on both enzymes. In addition, eugenol significantly (p < 0.05) had higher FRAP, ABTS scavenging, and Fe2+ chelating abilities when compared with the synthetic compounds (BHT and BHA). The strong inhibitory effects and antioxidative properties of eugenol suggest eugenol as a promising natural phenolic compound could be employed as an alternative food additive and erectogenic agent in ED management/treatment.

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Authors and Affiliations

  1. Functional Foods, Nutraceuticals and Phytomedicine Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria

    Stephen Adeniyi Adefegha, Bathlomew Maduka Okeke & Ganiyu Oboh

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  1. Stephen Adeniyi Adefegha
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  2. Bathlomew Maduka Okeke
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  3. Ganiyu Oboh
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Correspondence to Stephen Adeniyi Adefegha.

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Adefegha, S.A., Okeke, B.M. & Oboh, G. Comparative study on the interaction of eugenol, Butylated hydroxylanisole, and Butylated hydroxyl toluene with some crucial enzymes linked to erectile dysfunction. Comp Clin Pathol 27, 1699–1706 (2018). https://doi.org/10.1007/s00580-018-2796-0

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