Evaluation of antimalarial and biochemical profiles of Abaleria® in Plasmodium berghei-infected mice

Abstract

The rate of increasing resistance to most antimalarial drugs suggests a need for better alternatives. Hence, the present study evaluates the in vivo antimalarial and biochemical profiles of a locally formulated herbal antimalarial therapy, Abaleria® on mice infected with Plasmodium berghei. Eight groups of five mice each were used. The control groups include uninfected, infected with 1.0 × 107 P. berghei parasites but not treated, infected, and treated 3 days after inoculation with 25 mg kg−1 chloroquine diphosphate (CDP). Other groups were infected and treated with 50, 100, 200, 300, and 500 mg kg−1/day of Abaleria® for 4 days. On the 5th day, blood smears were prepared and evaluated for parasitemia microscopically, and animals were thereafter sacrificed; serum obtained from blood samples collected through cardiac puncture was used for biochemical assays. There was a significant (p < 0.05) reduction in parasitemia at the highest dose of the drug which compared favorably with CDP. Infection led to elevated liver function indices while treatment with Abaleria® normalized these parameters; a dose dependent increase in HDL-cholesterol was detected in the groups treated with Abaleria® and CDP. The study shows that Abaleria® displayed a dose-dependent in vivo antiplasmodial and biochemical properties as well as improvement of lipid profiles of mice infected with P. berghei.

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Acknowledgements

The authors appreciate the support of the technical staff of the Department of Biochemistry, Covenant University.

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Correspondence to Abiodun Humphrey Adebayo.

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The authors declare that they have no conflict of interest.

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The research was approved by the Ethics Committee of the Department of Biological Sciences Covenant University, Nigeria. All animals were also treated in line with the National institute of Health (NIH) guidelines for the use and care for animals in the laboratory (NIH 2011).

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Adebayo, A.H., Yakubu, O.F., Popoola, J.O. et al. Evaluation of antimalarial and biochemical profiles of Abaleria® in Plasmodium berghei-infected mice. Comp Clin Pathol 27, 1595–1601 (2018). https://doi.org/10.1007/s00580-018-2780-8

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Keywords

  • Abaleria®
  • Malaria
  • In vivo antimalarial studies
  • Plasmodium berghei
  • Biochemical profiles