Comparative Clinical Pathology

, Volume 27, Issue 5, pp 1205–1212 | Cite as

Studying embryotoxicity of petroleum products for carp fish family

  • Lina Lebedeva
  • Karakoz Mukhambetiyar
  • Mariya Suvorova
Original Article


To study the embryotoxicity of the oil fraction Karakuduk locality (Kazakhstan, oil deposit) for zebrafish Danio rerio (family Cyprinidae), in the conditions of the experiment. The method of breeding fishes of Danio rerio, receiving, and incubating zebrafish in vitro. Experiments have been carried out on the development of Danio rerio embryos in the oil-bearing fraction (Karakuduk locality) in various in vitro concentrations. The paper presents the results of experiments on exposure zebrafish embryos (Danio rerio) oil fractions in different concentrations and shows data on mortality of embryos and embryonic development violations. The originality of studying embryotoxicity of petroleum products for Cyprinidae fish family is that the object of our experiment is the most vulnerable age group in the population-fish larvae. Due to investigations, number of eggs could exactly determine the vitality of the fish population in general and can counter the number of the population through a certain period. Following the epidemic of mass mortality of caviar and larvae from oil pollution, the toxicant acts in the role of the immunosuppressive agent. Thus, it leads to the reduction of resistance to various infections with the reduction of the variability of even slight temperature and salinity fluctuations.


Oil Water-soluble fractions of oil Embryotoxicity Carp Danio rerio Embryos 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Informed consent

Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Molecular biology and genetics departmentAl-Farabi Kazakh National UniversityAlmatyRepublic of Kazakhstan

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